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Factors that influence fibrin clot properties and stroke are not fully understood. To study sulfur-containing amino acid metabolites, fibrin clot lysis time (CLT) and maximum absorbance (Abs<jats:sub>max<\/jats:sub>) in relation to stroke, we analyzed plasma and urine from 191 stroke patients (45.0% women, age 68\u2009\u00b1\u200912\u00a0years) and 291 healthy individuals (59.7% women, age 50\u2009\u00b1\u200917\u00a0years). Plasma and urinary levels of sulfur-containing amino acid metabolites and fibrin clot properties were significantly different in stroke patients compared to healthy individuals. Fibrin CLT correlated with fibrin Abs<jats:sub>max<\/jats:sub> in healthy males (R<jats:sup>2<\/jats:sup>\u2009=\u20090.439, <jats:italic>P<\/jats:italic>\u2009=\u20090.000), females (R<jats:sup>2<\/jats:sup>\u2009=\u20090.245, <jats:italic>P<\/jats:italic>\u2009=\u20090.000), female stroke patients (R<jats:sup>2<\/jats:sup>\u2009=\u20090.187, <jats:italic>P<\/jats:italic>\u2009=\u20090.000), but not in male stroke patients (R<jats:sup>2<\/jats:sup>\u2009=\u20090.008, <jats:italic>P<\/jats:italic>\u2009=\u2009ns). Fibrin CLT correlated with age in healthy females but not males while fibrin Abs<jats:sub>max<\/jats:sub> correlated with age in both sexes; these correlations were absent in stroke patients. In multiple regression analysis in stroke patients, plasma (p)CysGly, pMet, and <jats:italic>MTHFR A1298C<\/jats:italic> polymorphism were associated with fibrin Abs<jats:sub>max<\/jats:sub>, while urinary (u)HTL, uCysGly, and pCysGly were significantly associated with fibrin CLT. In healthy individuals, uHTL and uGSH were significantly associated with fibrin Abs<jats:sub>max<\/jats:sub>, while pGSH, and <jats:italic>CBS T833C 844ins68<\/jats:italic> polymorphism were associated with fibrin CLT. In logistic regression, uHTL, uHcy, pCysGly, pGSH, <jats:italic>MTHFR C677T<\/jats:italic> polymorphism, and Abs<jats:sub>max<\/jats:sub> were independently associated with stroke. Our findings suggest that HTL and other sulfur-containing amino acid metabolites influence fibrin clot properties and the risk of stroke.<\/jats:p>","DOI":"10.1038\/s41598-024-60706-2","type":"journal-article","created":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T13:02:08Z","timestamp":1715864528000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Homocysteine thiolactone and other sulfur-containing amino acid metabolites are associated with fibrin clot properties and the risk of ischemic stroke"],"prefix":"10.1038","volume":"14","author":[{"given":"Marta","family":"Sikora","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Bretes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Per\u0142a-Kaj\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Utyro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamila","family":"Borowczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justyna","family":"Piechocka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafa\u0142","family":"G\u0142owacki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izabela","family":"Wojtasz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rados\u0142aw","family":"Ka\u017amierski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hieronim","family":"Jakubowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,16]]},"reference":[{"key":"60706_CR1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000001209","author":"SS Martin","year":"2024","unstructured":"Martin, S. 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