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Proper lameness diagnosis aims to discover the structure provoking lameness. Ultrasonography documents valuable information of soft tissues and characterizes soft tissue injuries that have heretofore been difficult to obtain either noninvasively or via radiography. The objectives of the current study were to develop and describe a standardized ultrasonographic protocol for investigation of the tarsal region in donkeys. The donkey tarsal anatomy was investigated in 5 cadavers and the tarsi of 11 healthy lameness free adult donkeys were echographically investigated. The dorsal, plantar, lateral and medial aspects of the tarsal region were substantially evaluated at four anatomical landmarks in both the longitudinal and horizontal planes using a multi-frequency 5\u201312\u00a0MHz linear transducer. Sonoanatomy of the extensor and flexor tarsal tendons, collateral and plantar ligaments, and synovial pouches was delineated and described. Systematic echography of the tarsal region allowed accurate localization and thorough exploration of various soft tissues of clinical interest in the donkey tarsus. Sonograms provided in this study should serve as a reference database for tarsal ultrasonography in clinical circumstances.<\/jats:p>","DOI":"10.1038\/s41598-024-61066-7","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T00:01:41Z","timestamp":1715040101000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ultrasonography of the normal donkey tarsus (equus asinus)"],"prefix":"10.1038","volume":"14","author":[{"given":"Zakriya","family":"Almohamad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,7]]},"reference":[{"key":"61066_CR1","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1111\/evj.12231","volume":"46","author":"CE Reix","year":"2014","unstructured":"Reix, C. E., Burn, C. C., Pritchard, J. C., Barr, A. R. S. & Whay, H. R. 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