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Animals need to be maintained as heterozygous mutants, and homozygous mutant embryos yield only one-fourth of all embryos. In this study, we generated organ-deficient embryos using the CRISPR-Cas9-sgRNA\n                    <jats:sup>ms<\/jats:sup>\n                    system that induces cell death with a single-guide RNA (sgRNA\n                    <jats:sup>ms<\/jats:sup>\n                    ) targeting multiple sites in the genome. The Cas9-sgRNA\n                    <jats:sup>ms<\/jats:sup>\n                    system interrupted cell proliferation and induced cell ablation in vitro. The mouse model had Cas9 driven by the\n                    <jats:italic>Foxn1<\/jats:italic>\n                    promoter with a ubiquitous expression cassette of sgRNA\n                    <jats:sup>ms<\/jats:sup>\n                    at the\n                    <jats:italic>Rosa26<\/jats:italic>\n                    locus (\n                    <jats:italic>Foxn1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>Cas9<\/jats:italic>\n                    <\/jats:sup>\n                    ;\n                    <jats:italic>Rosa26_ms<\/jats:italic>\n                    ). It showed an athymic phenotype similar to that of nude mice but was not hairless. Eventually, a rat cell-derived thymus in an interspecies\u00a0chimera was generated by blastocyst complementation of\n                    <jats:italic>Foxn1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>Cas9<\/jats:italic>\n                    <\/jats:sup>\n                    ;\n                    <jats:italic>Rosa26_ms<\/jats:italic>\n                    mouse embryos with rat embryonic stem cells. Theoretically, a half of the total embryos has the Cas9-sgRNA\n                    <jats:sup>ms<\/jats:sup>\n                    system because Rosa26_ms could be maintained as homozygous.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-61167-3","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T03:02:36Z","timestamp":1715223756000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Generating an organ-deficient animal model using a multi-targeted CRISPR-Cas9 system"],"prefix":"10.1038","volume":"14","author":[{"given":"Jonathan Jun-Yong","family":"Lim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamato","family":"Murata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Yuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohei","family":"Kitamuro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Kawai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayako","family":"Isotani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,9]]},"reference":[{"key":"61167_CR1","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1016\/j.cell.2010.07.039","volume":"142","author":"T Kobayashi","year":"2010","unstructured":"Kobayashi, T. et al. 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