{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T05:03:33Z","timestamp":1749445413772,"version":"3.37.3"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/04413\/2020","2022.07931.PTDC"],"award-info":[{"award-number":["UID\/04413\/2020","2022.07931.PTDC"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agencia Espa\u00f1ola de Cooperacion Internacional para el Desarollo"},{"DOI":"10.13039\/501100005635","name":"Funda\u00e7\u00e3o Calouste Gulbenkian","doi-asserted-by":"publisher","award":["145278"],"award-info":[{"award-number":["145278"]}],"id":[{"id":"10.13039\/501100005635","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Staphylococcus aureus<\/jats:italic> is a frequent agent of bacteraemia. This bacterium has a variety of virulence traits that allow the establishment and maintenance of infection. This study explored the virulence profile of <jats:italic>S. aureus<\/jats:italic> strains causing paediatric bacteraemia (SAB) in Manhi\u00e7a district, Mozambique. We analysed 336 <jats:italic>S. aureus<\/jats:italic> strains isolated from blood cultures of children younger than 5 years admitted to the Manhi\u00e7a District Hospital between 2001 and 2019, previously characterized for antibiotic susceptibility and clonality. The strains virulence potential was evaluated by PCR detection of the Panton-Valentine leucocidin (PVL) encoding genes, <jats:italic>lukS-PV\/lukF-PV<\/jats:italic>, assessment of the capacity for biofilm formation and pathogenicity assays in <jats:italic>Galleria mellonella<\/jats:italic>. The overall carriage of PVL-encoding genes was over 40%, although reaching\u2009~\u200970 to 100% in the last years (2014 to 2019), potentially linked to the emergence of CC152 lineage. Strong biofilm production was a frequent trait of CC152 strains. Representative CC152 and CC121 strains showed higher virulence potential in the <jats:italic>G. mellonella<\/jats:italic> model when compared to reference strains, with variations within and between CCs. Our results highlight the importance of monitoring the emergent CC152-MSSA-PVL<jats:sup>+<\/jats:sup> and other lineages, as they display important virulence traits that may negatively impact the management of SAB paediatric patients in Manhi\u00e7a district, Mozambique.<\/jats:p>","DOI":"10.1038\/s41598-024-61345-3","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T15:01:58Z","timestamp":1715353318000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring the virulence potential of Staphylococcus aureus CC121 and CC152 lineages related to paediatric community-acquired bacteraemia in Manhi\u00e7a, Mozambique"],"prefix":"10.1038","volume":"14","author":[{"given":"Marcelino","family":"Garrine","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joana","family":"Neves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In\u00e1cio","family":"Mandomando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Couto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia Santos","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,10]]},"reference":[{"key":"61345_CR1","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1016\/j.cmi.2022.03.015","volume":"28","author":"AD Bai","year":"2022","unstructured":"Bai, A. D. et al. Staphylococcus aureus bacteraemia mortality: A systematic review and meta-analysis. Clin. Microbiol. Infect. 28, 1076\u20131084. https:\/\/doi.org\/10.1016\/j.cmi.2022.03.015 (2022).","journal-title":"Clin. Microbiol. Infect."},{"key":"61345_CR2","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.mib.2013.11.004","volume":"17","author":"M Otto","year":"2014","unstructured":"Otto, M. Staphylococcus aureus toxins. Curr. Opin. Microbiol. 17, 32\u201337. https:\/\/doi.org\/10.1016\/j.mib.2013.11.004 (2014).","journal-title":"Curr. Opin. Microbiol."},{"key":"61345_CR3","doi-asserted-by":"publisher","first-page":"677","DOI":"10.3390\/toxins13100677","volume":"13","author":"N Ahmad-Mansour","year":"2021","unstructured":"Ahmad-Mansour, N. et al. Staphylococcus aureus toxins: An update on their pathogenic properties and potential treatments. Toxins (Basel) 13, 677. https:\/\/doi.org\/10.3390\/toxins13100677 (2021).","journal-title":"Toxins (Basel)"},{"key":"61345_CR4","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.ijantimicag.2017.11.002","volume":"51","author":"K Saeed","year":"2018","unstructured":"Saeed, K. et al. Panton-Valentine leukocidin-positive Staphylococcus aureus: a position statement from the International Society of Chemotherapy. Int. J. Antimicrob. Agents. 51, 16\u201325. https:\/\/doi.org\/10.1016\/j.ijantimicag.2017.11.002 (2018).","journal-title":"Int. J. Antimicrob. Agents."},{"key":"61345_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/microbiolspec.GPP3-0039-2018","volume":"7","author":"K Tam","year":"2019","unstructured":"Tam, K. & Torres, V. J. Staphylococcus aureus secreted toxins and extracellular enzymes. Microbiol. Spectr. 7, 1\u201359. https:\/\/doi.org\/10.1128\/microbiolspec.GPP3-0039-2018 (2019).","journal-title":"Microbiol. 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