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Quinolone resistance is an emerging resistance among <jats:italic>E. coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic>. Therefore, the present study aimed to study the presence of plasmid-mediated quinolone resistance (<jats:italic>PMQR<\/jats:italic>) genes <jats:italic>qnrA, qnrB,<\/jats:italic> and <jats:italic>qnrS<\/jats:italic> by polymerase chain reaction (PCR) in <jats:italic>E. coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic> isolated from pediatric patients with sepsis. This was a retrospective cross-sectional study that included pediatric patients with healthcare-associated sepsis. The <jats:italic>E. coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic> isolates were identified by microbiological methods. <jats:italic>PMQR<\/jats:italic> genes namely <jats:italic>qnrA, qnrB,<\/jats:italic> and <jats:italic>qnrS<\/jats:italic> were detected in <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic> isolates by PCR. The results were analyzed by SPPS24, and the qualitative data was analyzed as numbers and percentages and comparison was performed by Chi-square test, P was significant if\u2009&lt;\u20090.05. The most prevalent gene detected by PCR was <jats:italic>qnrA<\/jats:italic> (75%), followed by <jats:italic>qnrB<\/jats:italic> (28.1%), and <jats:italic>qnrS<\/jats:italic> (25%). The most frequently detected <jats:italic>qnr<\/jats:italic> gene in <jats:italic>E coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic> was <jats:italic>qnrA<\/jats:italic> (28.8%, and 16.3% respectively). The present study highlights the high prevalence of ciprofloxacin resistance among <jats:italic>E. coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic> isolated from pediatric patients with healthcare-associated sepsis. There was a high frequency of <jats:italic>PMQR<\/jats:italic> genes in <jats:italic>E. coli<\/jats:italic> and <jats:italic>K. pneumoniae<\/jats:italic> isolated from pediatric patients. Therefore, it is important to monitor the spread of <jats:italic>PMQR<\/jats:italic> genes in clinical isolates to ensure efficient antibiotic use in those children. The finding denotes the importance of an antibiotics surveillance program.<\/jats:p>","DOI":"10.1038\/s41598-024-61357-z","type":"journal-article","created":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T03:43:18Z","timestamp":1716522198000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Study of plasmid mediated quinolone resistance genes among Escherichia coli and Klebsiella pneumoniae isolated from pediatric patients with sepsis"],"prefix":"10.1038","volume":"14","author":[{"given":"Ahmed","family":"Gomaa Elsayed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehab","family":"M Fahmy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Abdellatif Alsayed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai Essam","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maysaa","family":"El Sayed Zaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Mofreh Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,24]]},"reference":[{"key":"61357_CR1","doi-asserted-by":"publisher","first-page":"1207","DOI":"10.1016\/j.ajic.2014.07.020","volume":"42","author":"MA Gadallah","year":"2014","unstructured":"Gadallah, M. 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