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Brain diseases and brain functional abnormalities often involve multiple functionally connected regions, including the deep brain. Studies have shown that multi-target electrical stimulation is more effective than single-target electrical stimulation. However, non-invasive multi-target electromagnetic stimulation, such as multi-target transcranial magnetic stimulation (TMS), transcranial direct current stimulation (tDCS) and transcranial alternating current stimulation (tACS) cannot meet the needs of synchronous multi-target accurate electrical stimulation at the deep brain. In this paper, based on the principle of magneto-acoustic coupling and phased array focusing technology, a novel non-invasive multi-target transcranial magneto-acoustic coupling electrical stimulation (multi-target TMAES) method is proposed. A simulation model and experimental system were established. The simulation and experimental results proved that the proposed multi-target TMAES can non-invasively achieve precise focused electrical stimulation of two targets. The average focal point size\u00a0of each target is 5.1\u00a0mm. The location and intensity of the multi-target electrical stimulation can be flexibly changed by adjusting the system parameters according to the actual need. It will provide a new and promising tool for the treatment of brain diseases and the study of neural circuits and brain functional connectivity.<\/jats:p>","DOI":"10.1038\/s41598-024-61571-9","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T04:48:01Z","timestamp":1744346881000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The simulation and experimental validation of a novel noninvasive multi-target electrical stimulation method"],"prefix":"10.1038","volume":"15","author":[{"given":"Kai","family":"Zhu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingpeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunqi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhipeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"61571_CR1","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1146\/annurev-neuro-071013-014030","volume":"38","author":"ME Raichle","year":"2015","unstructured":"Raichle, M. 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