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Various chemical modifications of NAG have been made to promote its biomedical applications. In this study, we synthesized two bi-deoxygenated NAG, BNAG1 and BNAG2 and investigated their anti-inflammatory properties, using an in vivo and in vitro inflammation mouse model induced by lipopolysaccharide (LPS). Among the parent molecule NAG, BNAG1 and BNAG2, BNAG1 showed the highest inhibition against serum levels of IL-6 and TNF \u03b1 and the leukocyte migration to lungs and peritoneal cavity in LPS challenged mice, as well as IL-6 and TNF \u03b1 production in LPS-stimulated primary peritoneal macrophages. BNAG2 displayed an anti-inflammatory effect which was comparable to NAG. These findings implied potential application of these novel NAG derivatives, especially BNAG1, in treatment of certain inflammation-related diseases.<\/jats:p>","DOI":"10.1038\/s41598-024-61780-2","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T22:01:32Z","timestamp":1715724092000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Synthesis and anti-inflammatory activities of two new N-acetyl glucosamine derivatives"],"prefix":"10.1038","volume":"14","author":[{"given":"Zhichang","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weicheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanjun","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameer E.","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,14]]},"reference":[{"key":"61780_CR1","doi-asserted-by":"publisher","first-page":"826","DOI":"10.1038\/ni.3790","volume":"18","author":"MG Netea","year":"2017","unstructured":"Netea, M. 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A.E.H. has filed provisional patents for NAG as an antiviral treatment band for BNAG1 and BNAG 2 as an antiviral and anti-inflammatory treatment. A.E.H. reports consulting for Medtronic, Microvention, Stryker, Penumbra, Cerenovus, Genentech, GE Healthcare, Scientia, Balt, Viz.ai, Insera therapeutics, Proximie, NovaSignal, and Vesalio; acting as principal investigator for the COMPLETE study funded by Penumbra, and LVO SYNCHRONISE funded by Viz.ai; participating in the steering committee\/publication committee member for SELECT, DAWN, SELECT 2, EXPEDITE II, EMBOLISE, and CLEAR trials.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"11079"}}