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Integration of phytochemicals in nanoformulation might have higher applicability in healthcare. Current work demonstrates the synthesis of green AgNPs with\n                    <jats:italic>O. gratissimum<\/jats:italic>\n                    (gr-AgNPs)\n                    <jats:italic>O. tenuiflorum<\/jats:italic>\n                    (te-AgNPs) and\n                    <jats:italic>O. americanum<\/jats:italic>\n                    (am-AgNPs) followed by an evaluation of their antimicrobial and anticancer properties. SEM analysis revealed spherical-shaped particles with average particle sizes of 69.0\u2009\u00b1\u20095\u00a0nm for te-AgNPs, 46.9\u2009\u00b1\u20099\u00a0nm for gr-AgNPs, and 58.5\u2009\u00b1\u200918.7\u00a0nm for am-AgNPs with a polydispersity index below 0.4. The synthesized am-AgNPs effectively inhibited\n                    <jats:italic>Klebsiella pneumonia<\/jats:italic>\n                    <jats:italic>,<\/jats:italic>\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    <jats:italic>,<\/jats:italic>\n                    <jats:italic>Staphylococcus aureus, Aspergillus niger,<\/jats:italic>\n                    and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    with 23\u2009\u00b1\u20091.58\u00a0mm, 20\u2009\u00b1\u20091.68\u00a0mm, 22\u2009\u00b1\u20091.80\u00a0mm, 26\u2009\u00b1\u20091.85\u00a0mm, and 22\u2009\u00b1\u20091.40\u00a0nm of zone of inhibition respectively\n                    <jats:italic>.<\/jats:italic>\n                    Synthesized AgNPs also induced apoptotic cell death in MCF-7 in concentration-dependent manner. IC\n                    <jats:sub>50<\/jats:sub>\n                    values for am-AgNPs, te-AgNPs, and gr-AgNPs were 14.78\u2009\u00b1\u20090.89\u00a0\u00b5g, 18.04\u2009\u00b1\u20090.63 and 15.41\u2009\u00b1\u20090.37\u00a0\u00b5g respectively which suggested that am-AgNPs were the most effective against cancer. At higher dose size (20\u00a0\u00b5g) AgNPs were equally effective to commercial standard Doxorubicin (DOX). In comparison to te-AgNPs and gr-AgNPs, am-AgNPs have higher in vitro anticancer and antimicrobial effects. The work reported\n                    <jats:italic>Ocimum americanum<\/jats:italic>\n                    for its anticancer properties with chemical profile (GCMS) and compared it with earlier reported species. The activity against microbial pathogens and selected cancer cells clearly depicted that these species have distinct variations in activity. The results have also emphasized on higher potential of biogenic silver nanoparticles in healthcare but before formulation of commercial products, detailed analysis is required with human and animal models.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-61946-y","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T10:02:24Z","timestamp":1716372144000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Green synthesis of silver nanoparticle prepared with Ocimum species and assessment of anticancer potential"],"prefix":"10.1038","volume":"14","author":[{"given":"Asha Monica","family":"Alex","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senthilkumar","family":"Subburaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikha","family":"Chauhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishal","family":"Ahuja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholamreza","family":"Abdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam Abbasi","family":"Tarighat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,22]]},"reference":[{"key":"61946_CR1","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1080\/13102818.2016.1267594","volume":"31","author":"TA Abalkhil","year":"2017","unstructured":"Abalkhil, T. 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