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A major concern regarding this disease is drug resistance, which is increasingly reported worldwide. Hence, the discovery of a novel drug as well as drug targets is crucially required. Therefore, this study aims to characterize the novel drug target in the adult <jats:italic>F. gigantica<\/jats:italic>. In the beginning, we hypothesized that the parasite might interact with some host molecules when it lives inside the liver parenchyma or bile ducts, specifically hormones and hormone-like molecules, through the specific receptors, primarily nuclear receptors (NRs), which are recognized as a major drug target in various diseases. The retinoid X receptor (RXR) is a member of subfamily 2 NRs that plays multitudinous roles in organisms by forming homodimers or heterodimers with other NRs. We obtained the full-length amino acid sequences of <jats:italic>F. gigantica<\/jats:italic> retinoid X receptor-alpha (<jats:italic>Fg<\/jats:italic>RXR\u03b1-A) from the transcriptome of <jats:italic>F. gigantica<\/jats:italic> that existed in the NCBI database. The <jats:italic>Fg<\/jats:italic>RXR\u03b1-A were computationally predicted for the basic properties, multiple aligned, phylogeny analyzed, and generated of 2D and 3D models. Moreover, <jats:italic>Fg<\/jats:italic>RXR\u03b1-A was molecular cloned and expressed as a recombinant protein (r<jats:italic>Fg<\/jats:italic>RXR\u03b1-A), then used for immunization for specific polyclonal antibodies. The native <jats:italic>Fg<\/jats:italic>RXR\u03b1-A was detected in the parasite extracts and tissues, and the function was investigated by in vitro binding assay. The results demonstrated the conservation of <jats:italic>Fg<\/jats:italic>RXR\u03b1-A to the other RXRs, especially RXRs from the trematodes. Interestingly, the native <jats:italic>Fg<\/jats:italic>RXR\u03b1-A could be detected in the testes of the parasite, where the sex hormones are accumulated. Moreover, the binding assay revealed the interaction of 9-<jats:italic>cis<\/jats:italic> retinoic acid and <jats:italic>Fg<\/jats:italic>RXR\u03b1-A, suggesting the function of <jats:italic>Fg<\/jats:italic>RXR\u03b1-A. Our findings suggested that <jats:italic>Fg<\/jats:italic>RXR\u03b1-A will be involved with the sexual reproduction of the parasite by forming heterodimers with other NRs, and it could be the potential target for further drug development of fascioliasis.<\/jats:p>","DOI":"10.1038\/s41598-024-63194-6","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T13:02:57Z","timestamp":1716987777000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular and biochemical characterizations of a Fasciola gigantica retinoid X receptor-\u03b1 isoform A (FgRXR\u03b1-A)"],"prefix":"10.1038","volume":"14","author":[{"given":"Nattaya","family":"Torungkitmangmi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pathanin","family":"Chantree","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salisa","family":"Chaimon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parisa","family":"Prathaphan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jittiporn","family":"Ruangtong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amornrat","family":"Geadkaew-Krenc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phornphan","family":"Sornchuer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bumpenporn","family":"Sanannam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nattaya","family":"Thongsepee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viriya","family":"Pankao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poom","family":"Adisakwattana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pongsakorn","family":"Martviset","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,29]]},"reference":[{"key":"63194_CR1","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1186\/s13643-022-02013-3","volume":"11","author":"V Hoang Quang","year":"2022","unstructured":"Hoang Quang, V. et al. 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