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Its control efforts are hampered by widespread insecticide resistance reported in the Americas and Asia, while data from Africa is more limited. Here we use publicly available 729 <jats:italic>Ae. aegypti<\/jats:italic> whole-genome sequencing samples from 15 countries, including nine in Africa, to investigate the genetic diversity in four insecticide resistance linked genes: <jats:italic>ace-1<\/jats:italic>, <jats:italic>GSTe2<\/jats:italic>, <jats:italic>rdl<\/jats:italic> and <jats:italic>vgsc<\/jats:italic>. Apart from <jats:italic>vgsc<\/jats:italic>, the other genes have been less investigated in <jats:italic>Ae. aegypti<\/jats:italic>, and almost no genetic diversity information is available. Among the four genes, we identified 1,829 genetic variants including 474 non-synonymous substitutions, some of which have been previously documented, as well as putative copy number variations in <jats:italic>GSTe2<\/jats:italic> and <jats:italic>vgsc<\/jats:italic>. Global insecticide resistance phenotypic data demonstrated variable resistance in geographic areas with resistant genotypes. Overall, our work provides the first global catalogue and geographic distribution of known and new amino-acid mutations and duplications that can be used to guide the identification of resistance drivers in <jats:italic>Ae. aegypti<\/jats:italic> and thereby support monitoring efforts and strategies for vector control.<\/jats:p>","DOI":"10.1038\/s41598-024-64007-6","type":"journal-article","created":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T18:04:14Z","timestamp":1718129054000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Uncovering the genetic diversity in Aedes aegypti insecticide resistance genes through global comparative genomics"],"prefix":"10.1038","volume":"14","author":[{"given":"Anton","family":"Spadar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louisa A.","family":"Messenger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taane G.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Campino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,11]]},"reference":[{"key":"64007_CR1","first-page":"71","volume-title":"Globe-Trotting Aedes aegypti and Aedes albopictus: Risk Factors for Arbovirus Pandemics","author":"OW Lwande","year":"2020","unstructured":"Lwande, O. 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