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In these areas, antibiotic consumption rises which may impact both mosquito microbiota and dengue transmission. Here, we assessed how the ingestion by <jats:italic>Ae. aegypti<\/jats:italic> of therapeutic concentrations of amoxicillin\u2013clavulanic Acid association (Amox\/Clav), a broad-spectrum antibiotic used to treat febrile symptoms worldwide, impacted its microbiota. We also evaluated whether simultaneous ingestion of antibiotic and DENV impacted <jats:italic>Ae. aegypti<\/jats:italic> ability to transmit this virus. We found that Amox\/Clav ingestion impacted microbiota composition in <jats:italic>Ae. aegypti<\/jats:italic> and we confirmed such impact in field-collected mosquitoes. Furthermore, we observed that Amox\/Clav ingestion enhanced DENV dissemination and transmission by this mosquito at 21\u00a0days post-DENV exposure. These findings increase our understanding of factors linked to human hosts that may influence dengue transmission dynamics in regions with mass-drug administration programs.<\/jats:p>","DOI":"10.1038\/s41598-024-64221-2","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T21:03:55Z","timestamp":1718312635000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ingestion of amoxicillin\u2013clavulanic acid at therapeutic concentration during blood meal impacts Aedes aegypti microbiota and dengue virus transmission"],"prefix":"10.1038","volume":"14","author":[{"given":"Margot","family":"Garcia--Van Sm\u00e9voorde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie","family":"Calvez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaure","family":"Qu\u00e9tel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Dollin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Breurec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anubis","family":"Vega-R\u00faa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,13]]},"reference":[{"key":"64221_CR1","unstructured":"WHO. 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