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In this study, we used aspirin to prevent AOM\/DSS-induced CRC in mice, and the anti-CRC efficacy of aspirin was assessed using haematoxylin and eosin (H&amp;E) staining and by determining the mouse survival rate and tumour size. 16S rDNA sequencing, flow cytometry (FCM), and Western blotting were also conducted to investigate the changes in the gut microbiota, tumour immune microenvironment, and apoptotic proteins, respectively. The results demonstrated that aspirin significantly exerted anti-CRC effects in mice. According to 16S rDNA sequencing, aspirin regulated the composition of the gut microbiota and dramatically reduced the abundance of <jats:italic>Enterococcus cecorum<\/jats:italic>. FCM demonstrated that there were more CD155 tumour cells and CD4\u2009+\u2009CD25\u2009+\u2009Treg cells showed increased TIGIT levels. Moreover, increased TIGIT expression on Treg cells is associated with reduced Treg cell functionality. Importantly, the inhibition of Treg cells is accompanied by the promotion of CD19\u2009+\u2009GL-7\u2009+\u2009B cells, CD8\u2009+\u2009T cells, CD4\u2009+\u2009CCR4\u2009+\u2009Th2 cells, and CD4\u2009+\u2009CCR6\u2009+\u2009Th17 cells. Overall, aspirin prevents colorectal cancer by regulating the abundance of <jats:italic>Enterococcus cecorum<\/jats:italic> and TIGIT\u2009+\u2009Treg cells.<\/jats:p>","DOI":"10.1038\/s41598-024-64447-0","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T19:01:39Z","timestamp":1718218899000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Aspirin prevents colorectal cancer by regulating the abundance of Enterococcus cecorum and TIGIT+Treg cells"],"prefix":"10.1038","volume":"14","author":[{"given":"Xiaojuan","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajuan","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengkui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhua","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxia","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangguo","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,12]]},"reference":[{"key":"64447_CR1","doi-asserted-by":"publisher","first-page":"219","DOI":"10.3390\/cancers14010219","volume":"14","author":"CW Lai","year":"2022","unstructured":"Lai, C. 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