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Developing strategies for effective clinical treatments requires the discovery of promyelinating drugs. Increased GABAergic signaling through \u03b3-aminobutyric acid type A receptor (GABA<jats:sub>A<\/jats:sub>R) activation in oligodendrocytes has been proposed as a promyelinating condition. GABA<jats:sub>A<\/jats:sub>R expressed in oligodendroglia is strongly potentiated by n-butyl-\u03b2-carboline-3-carboxylate (\u03b2-CCB) compared to that in neurons. Here, mice were subjected to 0.3% cuprizone (CPZ) added in the food to induce central nervous system demyelination, a well-known model for multiple sclerosis. Then \u03b2-CCB (1\u00a0mg\/Kg) was systemically administered to analyze the remyelination status in white and gray matter areas. Myelin content was evaluated using Black-Gold II (BGII) staining, immunofluorescence (IF), and magnetic resonance imaging (MRI). Evidence indicates that \u03b2-CCB treatment of CPZ-demyelinated animals promoted remyelination in several white matter structures, such as the <jats:italic>fimbria<\/jats:italic>, <jats:italic>corpus callosum<\/jats:italic>, <jats:italic>internal capsule<\/jats:italic>, and <jats:italic>cerebellar peduncles<\/jats:italic>. Moreover, using IF, it was observed that CPZ intake induced an increase in NG2<jats:sup>+<\/jats:sup> and a decrease in CC1<jats:sup>+<\/jats:sup> cell populations, alterations that were importantly retrieved by \u03b2-CCB treatment. Thus, the promyelinating character of \u03b2-CCB was confirmed in a generalized demyelination model, strengthening the idea that it has clinical potential as a therapeutic drug.<\/jats:p>","DOI":"10.1038\/s41598-024-64501-x","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T23:02:23Z","timestamp":1718665343000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["N-butyl-\u03b2-carboline-3-carboxylate (\u03b2-CCB) systemic administration promotes remyelination in the cuprizone demyelinating model in mice"],"prefix":"10.1038","volume":"14","author":[{"given":"Fidel","family":"V\u00e9lez-Uriza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainald Pablo","family":"Ordaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith","family":"Garay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham J.","family":"Cisneros-Mejorado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogelio O.","family":"Arellano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"key":"64501_CR1","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1038\/nn.3990","volume":"18","author":"M Zonouzi","year":"2015","unstructured":"Zonouzi, M. et al. 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