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However, the impact of <jats:italic>Salmonella<\/jats:italic> Typhimurium on stem cells and progenitors, which are responsible for the ability of the intestinal epithelium to renew and protect itself, remains unclear. Given that intestinal organoids growth is sustained by stem cells and progenitors activity, we have used this model to document the effects of <jats:italic>Salmonella<\/jats:italic> Typhimurium infection on epithelial proliferation and differentiation, and compared it to an in vivo model of <jats:italic>Salmonella<\/jats:italic> infection in mice. Among gut segments, the caecum was preferentially targeted by <jats:italic>Salmonella<\/jats:italic>. Analysis of infected crypts and organoids demonstrated increased length and size, respectively. mRNA transcription profiles of infected crypts and organoids pointed to upregulated EGFR-dependent signals, associated with a decrease in secretory cell lineage differentiation. To conclude, we show that organoids are suited to mimic the impact of <jats:italic>Salmonella<\/jats:italic> on stem cells and progenitors cells, carrying a great potential to drastically reduce the use of animals for scientific studies on that topic. In both models, the EGFR pathway, crucial to stem cells and progenitors proliferation and differentiation, is dysregulated by <jats:italic>Salmonella,<\/jats:italic> suggesting that repeated infections might have consequences on crypt integrity and further oncogenesis.<\/jats:p>","DOI":"10.1038\/s41598-024-65485-4","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T11:02:15Z","timestamp":1719918135000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Intestinal organoids to model Salmonella infection and its impact on progenitors"],"prefix":"10.1038","volume":"14","author":[{"given":"Jin","family":"Yan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Racaud-Sultan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Pezier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anissa","family":"Edir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Rolland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralie","family":"Claverie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Burlaud-Gaillard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Olivier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Velge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Lacroix-Lamand\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Vergnolle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s","family":"Wiedemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,2]]},"reference":[{"key":"65485_CR1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1038\/s41575-018-0081-y","volume":"16","author":"H Gehart","year":"2019","unstructured":"Gehart, H. & Clevers, H. 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