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Tanycytes, the adult stem cells in the hypothalamus, play crucial roles in assisting hypothalamic neurons in maintaining energy balance. Although tanycytes have been extensively studied in rodents, our understanding of human tanycytes remains limited. In this study, we utilized single-cell transcriptomics data to explore the heterogeneity of human embryonic tanycytes, investigate their gene regulatory networks, analyze their intercellular communication, and examine their developmental trajectory. Our analysis revealed the presence of two clusters of \u03b2 tanycytes and three clusters of \u03b1 tanycytes in our dataset. Surprisingly, human embryonic tanycytes displayed significant similarities to mouse tanycytes in terms of marker gene expression and transcription factor activities. Trajectory analysis indicated that \u03b1 tanycytes were the first to be generated, giving rise to \u03b2 tanycytes in a dorsal\u2013ventral direction along the third ventricle. Furthermore, our CellChat analyses demonstrated that tanycytes generated earlier along the developmental lineages exhibited increased intercellular communication compared to those generated later. In summary, we have thoroughly characterized the heterogeneity of human embryonic tanycytes from various angles. We are confident that our findings will serve as a foundation for future research on human tanycytes.<\/jats:p>","DOI":"10.1038\/s41598-024-66044-7","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T09:02:42Z","timestamp":1720083762000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A single-cell transcriptomic study of heterogeneity in human embryonic tanycytes"],"prefix":"10.1038","volume":"14","author":[{"given":"Yiguang","family":"Bai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoling","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"key":"66044_CR1","doi-asserted-by":"publisher","first-page":"994","DOI":"10.1152\/ajpregu.00296.2020","volume":"320","author":"M Bolborea","year":"2021","unstructured":"Bolborea, M. & Langlet, F. 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