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Because orally administered LPS activates innate immunity without inducing inflammation, it plays a role as an active ingredient in herbal extracts. However, the LPS content in herbal extracts remains extensively unevaluated. This study aimed to create a database of LPS content in herbal extracts; therefore, the LPS content of 414 herbal extracts was measured and the macrophage activation potential was evaluated. The LPS content of these hot water extracts was determined using the kinetic\u2013turbidimetric method. The LPS concentration ranged from a few ng\/g to hundreds of \u03bcg\/g (Standard <jats:italic>Escherichia coli<\/jats:italic> LPS equivalent). Twelve samples had a high-LPS-content of\u2009&gt;\u2009100\u00a0\u03bcg\/g, including seven samples from roots and three samples from leaves of the herbal extracts. These samples showed high phagocytosis and NO production capacity, and further investigation using polymyxin B, an LPS inhibitor, significantly inhibited macrophage activation. This study suggests that some herbal extracts contain sufficient LPS concentration to activate innate immunity. Therefore, a new approach to evaluate the efficacy of herbal extracts based on their LPS content was proposed. A database listing the LPS content of different herbal extracts is essential for this approach.<\/jats:p>","DOI":"10.1038\/s41598-024-66081-2","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T12:01:48Z","timestamp":1720094508000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Baseline data collections of lipopolysaccharide content in 414 herbal extracts and its role in innate immune activation"],"prefix":"10.1038","volume":"14","author":[{"given":"Vindy","family":"Tjendana Tjhin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Oda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Iwaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuko","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Wakame","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Inagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gen-Ichiro","family":"Soma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"key":"66081_CR1","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1016\/S1286-4579(02)01604-0","volume":"4","author":"C Erridge","year":"2002","unstructured":"Erridge, C., Bennett-Guerrero, E. & Poxton, I. 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