{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:50:04Z","timestamp":1792785004227,"version":"4.5.0"},"reference-count":67,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T00:00:00Z","timestamp":1721692800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T00:00:00Z","timestamp":1721692800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge."},{"DOI":"10.13039\/501100002385","name":"Ministry of Higher Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002385","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022025","name":"South Valley University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022025","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tick-borne diseases in animals are increasing rapidly worldwide, but there is insufficient information about tick-borne diseases infecting dogs in southern Egypt. Thus, in the current study, we detected the presence of\n                    <jats:italic>Anaplasma marginale (A. marginale)<\/jats:italic>\n                    and\n                    <jats:italic>Babesia canis vogeli<\/jats:italic>\n                    (\n                    <jats:italic>B. canis vogeli<\/jats:italic>\n                    ) in the blood of dogs. The results revealed that 4\/100 (4%) were positive, and a higher infection rate was found in males (75%), than females (25%). The phylogenetic analysis for the\n                    <jats:italic>major surface protein 4<\/jats:italic>\n                    (\n                    <jats:italic>msp4<\/jats:italic>\n                    ) gene in this study was compared with amplicons separate from other reported isolates with alignment by identity 100% with cattle and camels from Egypt, and the phylogenetic analysis for the\n                    <jats:italic>B. canis vogeli small subunit ribosomal RNA<\/jats:italic>\n                    (\n                    <jats:italic>SSU rRNA<\/jats:italic>\n                    ) gene in this study identified identity by 99.89% with dogs from Egypt. This report is considered the first report in southern Egypt about\n                    <jats:italic>A. marginale<\/jats:italic>\n                    in dogs based on the sequence analysis of the\n                    <jats:italic>msp4<\/jats:italic>\n                    gene, providing new data for the classification and identification of\n                    <jats:italic>A. marginale<\/jats:italic>\n                    in dogs compared to\n                    <jats:italic>A. marginale<\/jats:italic>\n                    isolated from other animals in southern Egypt.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-67009-6","type":"journal-article","created":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T04:01:52Z","timestamp":1721707312000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular detection and characterization of Anaplasma marginale and Babesia canis vogeli infecting dogs in Luxor, Egypt"],"prefix":"10.1038","volume":"14","author":[{"given":"Hassan Y. A. H.","family":"Mahmoud","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moshera S.","family":"Shahat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ragab M.","family":"Fereig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alsagher O.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walaa F. A.","family":"Emeish","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M.","family":"Soliman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma A.","family":"Khalifa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,23]]},"reference":[{"issue":"1","key":"67009_CR1","doi-asserted-by":"publisher","first-page":"18","DOI":"10.3201\/eid0201.960102","volume":"2","author":"DH Walker","year":"1996","unstructured":"Walker, D. H. & Dumler, J. S. Emergence of the ehrlichioses as human health problems. Emerg. Infect. Dis. 2(1), 18 (1996).","journal-title":"Emerg. Infect. Dis."},{"key":"67009_CR2","first-page":"103","volume":"9","author":"CSE Berm\u00fadez","year":"2018","unstructured":"Berm\u00fadez, C. S. E. & Troyo, A. A review of the genus Rickettsia in Central America. Res. Rep. Trop. Med. 9, 103\u2013112 (2018).","journal-title":"Res. Rep. Trop. Med."},{"issue":"2\u20134","key":"67009_CR3","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.vetpar.2009.09.012","volume":"167","author":"KM Kocan","year":"2010","unstructured":"Kocan, K. M., de la Fuente, J., Blouin, E. F., Coetzee, J. F. & Ewing, S. The natural history of Anaplasma marginale. Vet. Parasitol. 167(2\u20134), 95\u2013107 (2010).","journal-title":"Vet. Parasitol."},{"key":"67009_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-020-04288-8","volume":"13","author":"V-L Nguyen","year":"2020","unstructured":"Nguyen, V.-L. et al. Molecular detection of pathogens in ticks and fleas collected from companion dogs and cats in East and Southeast Asia. Parasites Vectors 13, 1\u201311 (2020).","journal-title":"Parasites Vectors"},{"issue":"9857","key":"67009_CR5","doi-asserted-by":"publisher","first-page":"1946","DOI":"10.1016\/S0140-6736(12)61151-9","volume":"380","author":"AM Kilpatrick","year":"2012","unstructured":"Kilpatrick, A. M. & Randolph, S. E. Drivers, dynamics, and control of emerging vector-borne zoonotic diseases. Lancet 380(9857), 1946\u20131955 (2012).","journal-title":"Lancet"},{"issue":"2","key":"67009_CR6","first-page":"151","volume":"14","author":"TG Gokmen","year":"2019","unstructured":"Gokmen, T. G. et al. A serosurvey on some canine vector-borne zoonoses (Anaplasma spp., Ehrlichia spp., Borrelia burgdorferi, Dirofilaria immitis and Leishmania spp.) in Osmaniye. J Atat\u00fcrk \u00dcniversitesi Veteriner Bilimleri Dergisi. 14(2), 151\u2013158 (2019).","journal-title":"J Atat\u00fcrk \u00dcniversitesi Veteriner Bilimleri Dergisi."},{"key":"67009_CR7","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.vetpar.2017.04.004","volume":"239","author":"SE Khatat","year":"2017","unstructured":"Khatat, S. E. et al. Detection of Anaplasma spp. and Ehrlichia spp. anibodies, and Dirofilaria immitis antigens in dogs from seven locations of Morocco. Vet. Parasitol. 239, 86\u201389 (2017).","journal-title":"Vet. Parasitol."},{"key":"67009_CR8","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.actatropica.2019.02.029","volume":"193","author":"B Salim","year":"2019","unstructured":"Salim, B. et al. Potential role of dogs as sentinels and reservoirs for piroplasms infecting equine and cattle in Riyadh City, Saudi Arabia. Acta Trop. 193, 78\u201383 (2019).","journal-title":"Acta Trop."},{"key":"67009_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13071-017-2092-x","volume":"10","author":"MO Andersson","year":"2017","unstructured":"Andersson, M. O. et al. Canine tick-borne diseases in pet dogs from Romania. Parasites Vectors 10, 1\u20136 (2017).","journal-title":"Parasites Vectors"},{"issue":"3","key":"67009_CR10","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/S0304-4017(03)00141-9","volume":"114","author":"A Criado-Fornelio","year":"2003","unstructured":"Criado-Fornelio, A., Martinez-Marcos, A., Buling-Sarana, A. & Barba-Carretero, J. Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe: Part II. Phylogenetic analysis and evolutionary history. Vet. Parasitol. 114(3), 173\u2013194 (2003).","journal-title":"Vet. Parasitol."},{"key":"67009_CR11","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1007\/s00436-010-2015-7","volume":"107","author":"O Cabez\u00f3n","year":"2010","unstructured":"Cabez\u00f3n, O. et al. Kennel dogs as sentinels of Leishmania infantum, Toxoplasma gondii, and Neospora caninum in Majorca Island, Spain. Parasitol. Res 107, 1505\u20131508 (2010).","journal-title":"Parasitol. Res"},{"issue":"4","key":"67009_CR12","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1186\/1756-3305-4-141","volume":"19","author":"PA Abd Rani","year":"2011","unstructured":"Abd Rani, P. A., Irwin, P. J., Coleman, G. T., Gatne, M. & Traub, R. J. A survey of canine tick-borne diseases in India. Parasit. Vectors 19(4), 141 (2011).","journal-title":"Parasit. Vectors"},{"issue":"7","key":"67009_CR13","doi-asserted-by":"publisher","first-page":"959","DOI":"10.2460\/javma.2003.222.959","volume":"222","author":"JR Stegeman","year":"2003","unstructured":"Stegeman, J. R., Birkenheuer, A. J., Kruger, J. M. & Breitschwerdt, E. B. Transfusion-associated Babesia gibsoni infection in a dog. J. Am. Vet. Med. Assoc. 222(7), 959\u2013963 (2003).","journal-title":"J. Am. Vet. Med. Assoc."},{"issue":"3","key":"67009_CR14","doi-asserted-by":"publisher","first-page":"500","DOI":"10.5604\/12321966.1120590","volume":"21","author":"EJ Mierzejewska","year":"2014","unstructured":"Mierzejewska, E. J., Welc-Faleciak, R., Bednarska, M., Rodo, A. & Bajer, A. The first evidence for vertical transmission of Babesia canis in a litter of Central Asian Shepherd dogs. Ann. Agric. Environ. Med. 21(3), 500\u2013503 (2014).","journal-title":"Ann. Agric. Environ. Med."},{"issue":"1","key":"67009_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1756-3305-4-50","volume":"4","author":"PB Sim\u00f5es","year":"2011","unstructured":"Sim\u00f5es, P. B., Cardoso, L., Ara\u00fajo, M., Yisaschar-Mekuzas, Y. & Baneth, G. Babesiosis due to the canine Babesia microti-like small piroplasm in dogs-first report from Portugal and possible vertical transmission. Parasit. Vectors 4(1), 1\u20136 (2011).","journal-title":"Parasit. Vectors"},{"issue":"2","key":"67009_CR16","doi-asserted-by":"publisher","first-page":"173","DOI":"10.56808\/2985-1130.2379","volume":"42","author":"K Buddhachat","year":"2012","unstructured":"Buddhachat, K., Meesong, O., Nganvongpanit, K., Osathanunkul, M. & Chomdej, S. Molecular characterization and detection of Babesia canis vogeli in asymptomatic roaming dogs in Chiang Mai, Thailand. Thai J. Vet. Med. 42(2), 173\u2013178 (2012).","journal-title":"Thai J. Vet. Med."},{"issue":"3\u20134","key":"67009_CR17","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.vetpar.2008.07.024","volume":"157","author":"L Solano-Gallego","year":"2008","unstructured":"Solano-Gallego, L. et al. Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease. Vet. Parasitol. 157(3\u20134), 211\u2013221 (2008).","journal-title":"Vet. Parasitol."},{"issue":"6","key":"67009_CR18","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1016\/j.cvsm.2010.08.001","volume":"40","author":"PJ Irwin","year":"2010","unstructured":"Irwin, P. J. Canine babesiosis. Vet. Clin. Small Anim. Pract. 40(6), 1141\u20131156 (2010).","journal-title":"Vet. Clin. Small Anim. Pract."},{"issue":"3","key":"67009_CR19","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.pt.2011.11.003","volume":"28","author":"V Matijatko","year":"2012","unstructured":"Matijatko, V., Torti, M. & Schetters, T. P. Canine babesiosis in Europe: How many diseases?. Trends Parasitol. 28(3), 99\u2013105 (2012).","journal-title":"Trends Parasitol."},{"key":"67009_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.vetpar.2018.02.028","volume":"253","author":"J Wang","year":"2018","unstructured":"Wang, J. et al. First description of the pathogenicity of Babesia vogeli in experimentally infected dogs. Vet. Parasitol. 253, 1\u20137 (2018).","journal-title":"Vet. Parasitol."},{"issue":"4","key":"67009_CR21","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1093\/jmedent\/42.4.668","volume":"42","author":"GA Scoles","year":"2005","unstructured":"Scoles, G. A., Broce, A. B., Lysyk, T. J. & Palmer, G. H. Relative efficiency of biological transmission of Anaplasma marginale (Rickettsiales: Anaplasmataceae) by Dermacentor andersoni (Acari: Ixodidae) compared with mechanical transmission by Stomoxys calcitrans (Diptera: Muscidae). J. Med. Entomol. 42(4), 668\u2013675 (2005).","journal-title":"J. Med. Entomol."},{"key":"67009_CR22","first-page":"767","volume-title":"Current Veterinary Therapy Food Animal Practice","author":"EJ Richey","year":"1981","unstructured":"Richey, E. J. Bovine anaplasmosis. In Current Veterinary Therapy Food Animal Practice (ed. Howard, R. J.) 767\u201372 (The W.B. Saunders Co, 1981)."},{"issue":"1","key":"67009_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1865-1682.2010.01173.x","volume":"58","author":"P Aubry","year":"2011","unstructured":"Aubry, P. & Geale, D. W. A review of bovine anaplasmosis. Transbound. Emerg. Dis. 58(1), 1\u201330 (2011).","journal-title":"Transbound. Emerg. Dis."},{"key":"67009_CR24","unstructured":"Ristic, M. Bovine anaplasmosis. In Kreier J. (Eds) Parasitic protozoa, pp. 235\u2013249 (1977)."},{"issue":"3","key":"67009_CR25","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.ttbdis.2015.03.007","volume":"6","author":"A Cabezas-Cruz","year":"2015","unstructured":"Cabezas-Cruz, A. & de la Fuente, J. Anaplasma marginale major surface protein 1a: A marker of strain diversity with implications for control of bovine anaplasmosis. Ticks Tick-Borne Dis. 6(3), 205\u2013210 (2015).","journal-title":"Ticks Tick-Borne Dis."},{"key":"67009_CR26","doi-asserted-by":"publisher","first-page":"1169323","DOI":"10.3389\/fvets.2023.1169323","volume":"10","author":"HYAH Mahmoud","year":"2023","unstructured":"Mahmoud, H. Y. A. H., Ali, A. O. & Tanaka, T. Molecular detection and characterization of Anaplasma marginale infecting cattle, buffalo, and camel populations in southern Egypt. Front. Vet. Sci. 10, 1169323 (2023).","journal-title":"Front. Vet. Sci."},{"key":"67009_CR27","volume-title":"Schalm's Veterinary Hematology","author":"NC Jain","year":"1986","unstructured":"Jain, N. C. Schalm\u2019s Veterinary Hematology 4th edn. (Lea and Febiger, 1986).","edition":"4"},{"issue":"3","key":"67009_CR28","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1007\/s10493-018-0218-2","volume":"74","author":"ML Kwak","year":"2018","unstructured":"Kwak, M. L. The introduction and subsequent extinction of the camel tick Hyalomma (Euhyalomma) dromedarii (Acari, Ixodidae) in Australia, with a review of the introduction of foreign ticks to Australia. Exp. Appl. Acarol. 74(3), 329\u2013333 (2018).","journal-title":"Exp. Appl. Acarol."},{"issue":"3","key":"67009_CR29","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1111\/mve.12502","volume":"35","author":"M Okely","year":"2021","unstructured":"Okely, M., Anan, R., Gad-Allah, S. & Samy, A. M. Hard ticks (Acari: Ixodidae) infesting domestic animals in Egypt: Diagnostic characters and a taxonomic key to the collected species. Med. Vet. Entomol. 35(3), 333\u2013351 (2021).","journal-title":"Med. Vet. Entomol."},{"issue":"21","key":"67009_CR30","doi-asserted-by":"publisher","first-page":"10034","DOI":"10.1073\/pnas.91.21.10034","volume":"91","author":"WC Black","year":"1994","unstructured":"Black, W. C. & Piesman, J. Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences. Proc. Natl. Acad. Sci. U. S. A. 91(21), 10034\u201310038 (1994).","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"12","key":"67009_CR31","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.vetpar.2007.12.013","volume":"152","author":"SC Duarte","year":"2008","unstructured":"Duarte, S. C., Linhares, G. F. C., Romanowsky, T. N., da Silveira Neto, O. J. & Borges, L. M. F. Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay. Vet. Parasitol. 152(12), 16\u201320 (2008).","journal-title":"Vet. Parasitol."},{"issue":"1","key":"67009_CR32","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/S0304-4017(01)00378-8","volume":"97","author":"J de la Fuente","year":"2001","unstructured":"de la Fuente, J., Van Den Bussche, R. A. & Kocan, K. M. Molecular phylogeny and biogeography of North American isolates of Anaplasma marginale (Rickettsiaceae: Ehrlichieae). Vet. Parasitol. 97(1), 65\u201376 (2001).","journal-title":"Vet. Parasitol."},{"issue":"6","key":"67009_CR33","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/molbev\/msy096","volume":"35","author":"S Kumar","year":"2018","unstructured":"Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35(6), 1547\u20131549 (2018).","journal-title":"Mol. Biol. Evol."},{"issue":"2","key":"67009_CR34","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1007\/BF01731581","volume":"16","author":"M Kimura","year":"1980","unstructured":"Kimura, M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16(2), 111\u2013120 (1980).","journal-title":"J. Mol. Evol."},{"issue":"8","key":"67009_CR35","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1002\/(SICI)1097-0258(19980430)17:8<857::AID-SIM777>3.0.CO;2-E","volume":"17","author":"RG Newcombe","year":"1998","unstructured":"Newcombe, R. G. Two-sided confidence intervals for the single proportion: Comparison of seven methods. Stat. Med. 17(8), 857\u2013872 (1998).","journal-title":"Stat. Med."},{"issue":"9","key":"67009_CR36","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1017\/S0031182007002594","volume":"134","author":"GO Aboge","year":"2007","unstructured":"Aboge, G. O. et al. Molecular characterization of a novel 32-kDa merozoite antigen of Babesia gibsoni with a better diagnostic performance by enzyme-linked immunosorbent assay. Parasitology 134(9), 1185\u20131194 (2007).","journal-title":"Parasitology"},{"issue":"6","key":"67009_CR37","doi-asserted-by":"publisher","first-page":"2465","DOI":"10.1128\/JCM.42.6.2465-2469.2004","volume":"42","author":"H Ikadai","year":"2004","unstructured":"Ikadai, H. et al. Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method. J. Clin. Microbiol. 42(6), 2465\u20132469 (2004).","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"67009_CR38","first-page":"163","volume":"9","author":"DW Yao","year":"2014","unstructured":"Yao, D. W. et al. Canine Babesiosis in China caused by Babesia gibsoni: A molecular approach. Iran. J. Parasitol. 9(2), 163\u2013168 (2014).","journal-title":"Iran. J. Parasitol."},{"issue":"1\u20132","key":"67009_CR39","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.vetpar.2006.01.035","volume":"138","author":"G Uilenberg","year":"2006","unstructured":"Uilenberg, G. Babesia\u2013a historical overview. Vet. Parasitol. 138(1\u20132), 3\u201310 (2006).","journal-title":"Vet. Parasitol."},{"issue":"6","key":"67009_CR40","doi-asserted-by":"publisher","first-page":"1046","DOI":"10.3390\/microorganisms12061046","volume":"12","author":"YJ Kim","year":"2024","unstructured":"Kim, Y. J. et al. Molecular analysis of tick-borne bacterial pathogens from ticks infesting animal hosts in Kyrgyzstan, 2021. Microorganisms 12(6), 1046 (2024).","journal-title":"Microorganisms"},{"issue":"1","key":"67009_CR41","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1186\/s13071-018-3192-y","volume":"11","author":"RL Galay","year":"2018","unstructured":"Galay, R. L. et al. Molecular detection of tick-borne pathogens in canine population and Rhipicephalus sanguineus (sensu lato) ticks from southern Metro Manila and Laguna, Philippines. Parasites Vectors 11(1), 643 (2018).","journal-title":"Parasites Vectors"},{"issue":"2","key":"67009_CR42","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.vetpar.2004.03.019","volume":"122","author":"P Matjila","year":"2004","unstructured":"Matjila, P., Penzhorn, B., Bekker, C., Nijhof, A. & Jongejan, F. Confirmation of occurrence of Babesia canis vogeli in domestic dogs in South Africa. Vet. Parasitol. 122(2), 119\u2013125 (2004).","journal-title":"Vet. Parasitol."},{"issue":"1\u20132","key":"67009_CR43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.vetpar.2007.12.018","volume":"152","author":"Y M\u2019ghirbi","year":"2008","unstructured":"M\u2019ghirbi, Y. & Bouattour, A. Detection and molecular characterization of Babesia canis vogeli from naturally infected dogs and Rhipicephalus sanguineus ticks in Tunisia. Vet. Parasitol. 152(1\u20132), 1\u20137 (2008).","journal-title":"Vet. Parasitol."},{"issue":"3\u20134","key":"67009_CR44","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.vetpar.2008.05.027","volume":"156","author":"L Cardoso","year":"2008","unstructured":"Cardoso, L. et al. Babesia canis canis and Babesia canis vogeli infections in dogs from northern Portugal. Vet. Parasitol. 156(3\u20134), 199\u2013204 (2008).","journal-title":"Vet. Parasitol."},{"issue":"3\u20134","key":"67009_CR45","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.vetpar.2012.01.030","volume":"187","author":"M Ren\u00e9","year":"2012","unstructured":"Ren\u00e9, M. et al. First evidence and molecular characterization of Babesia vogeli in naturally infected dogs and Rhipicephalus sanguineus ticks in southern France. Vet. Parasitol. 187(3\u20134), 399\u2013407 (2012).","journal-title":"Vet. Parasitol."},{"issue":"3\u20134","key":"67009_CR46","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.vetpar.2004.03.012","volume":"121","author":"H Inokuma","year":"2004","unstructured":"Inokuma, H. et al. Molecular survey of Babesia infection in dogs in Okinawa, Japan. Vet. Parasitol. 121(3\u20134), 341\u2013346 (2004).","journal-title":"Vet. Parasitol."},{"key":"67009_CR47","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.meegid.2015.01.011","volume":"31","author":"M Terao","year":"2015","unstructured":"Terao, M. et al. Molecular detection and genetic diversity of Babesia gibsoni in dogs in Bangladesh. Infect. Genet. Evol. 31, 53\u201360 (2015).","journal-title":"Infect. Genet. Evol."},{"issue":"2","key":"67009_CR48","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1645\/0022-3395(2003)089[0409:TSOCBI]2.0.CO;2","volume":"89","author":"R Jefferies","year":"2003","unstructured":"Jefferies, R., Ryan, U. M., Muhlnickel, C. J. & Irwin, P. J. Two species of canine Babesia in Australia: Detection and characterization by PCR. J. Parasitol. 89(2), 409\u2013412 (2003).","journal-title":"J. Parasitol."},{"issue":"1","key":"67009_CR49","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.exppara.2005.09.001","volume":"112","author":"AR Martin","year":"2006","unstructured":"Martin, A. R., Dunstan, R. H., Roberts, T. K. & Brown, G. K. Babesia canis vogeli: A novel PCR for its detection in dogs in Australia. Exp. Parasitol. 112(1), 63\u201365 (2006).","journal-title":"Exp. Parasitol."},{"issue":"3\u20134","key":"67009_CR50","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1016\/j.vetpar.2011.11.011","volume":"186","author":"G Vargas-Hern\u00e1ndez","year":"2012","unstructured":"Vargas-Hern\u00e1ndez, G. et al. Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia. Vet. Parasitol. 186(3\u20134), 254\u2013260 (2012).","journal-title":"Vet. Parasitol."},{"issue":"1","key":"67009_CR51","first-page":"21","volume":"17","author":"C Rey-Valeiron","year":"2007","unstructured":"Rey-Valeiron, C., Criado-Fornelio, A., Zavala, E. & Granados, R. Caracterizaci\u00f3n parasitol\u00f3gica y molecular de un aislado venezolano de Babesia canis. Rev. Cient. Fac. Cienc. Vet. 17(1), 21\u201328 (2007).","journal-title":"Rev. Cient. Fac. Cienc. Vet."},{"key":"67009_CR52","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1007\/s00436-010-1979-7","volume":"107","author":"R Ramos","year":"2010","unstructured":"Ramos, R. et al. Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (North-Eastern Brazil). Parasitol. Res. 107, 1115\u20131120 (2010).","journal-title":"Parasitol. Res."},{"issue":"3\u20134","key":"67009_CR53","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1016\/j.vetpar.2008.07.037","volume":"157","author":"DF Eiras","year":"2008","unstructured":"Eiras, D. F., Basabe, J., Mesplet, M. & Schnittger, L. First molecular characterization of Babesia vogeli in two naturally infected dogs of Buenos Aires, Argentina. J. Vet. Parasitol. 157(3\u20134), 294\u2013298 (2008).","journal-title":"J. Vet. Parasitol."},{"issue":"1","key":"67009_CR54","doi-asserted-by":"publisher","first-page":"6988","DOI":"10.1038\/s41598-022-11079-x","volume":"12","author":"A Selim","year":"2022","unstructured":"Selim, A., Megahed, A., Ben Said, M., Alanazi, A. D. & Sayed-Ahmed, M. Z. Molecular survey and phylogenetic analysis of Babesia vogeli in dogs. Sci. Rep. 12(1), 6988 (2022).","journal-title":"Sci. Rep."},{"issue":"3","key":"67009_CR55","doi-asserted-by":"publisher","first-page":"579","DOI":"10.14202\/vetworld.2020.579-585","volume":"13","author":"NM Badawi","year":"2020","unstructured":"Badawi, N. M. & Yousif, A. A. Babesia canis spp. in dogs in Baghdad Province, Iraq: First molecular identification and clinical and epidemiological study. Vet. World 13(3), 579 (2020).","journal-title":"Vet. World"},{"issue":"1","key":"67009_CR56","doi-asserted-by":"publisher","first-page":"163","DOI":"10.5433\/1679-0359.2016v37n1p163","volume":"37","author":"VCL da Silva","year":"2016","unstructured":"da Silva, V. C. L. et al. Parasitological and molecular detection of Babesia canis vogeli in dogs of Recife, Pernambuco and evaluation of risk factors associated. Semin. Ci\u00eanc. Ag. 37(1), 163\u2013171 (2016).","journal-title":"Semin. Ci\u00eanc. Ag."},{"key":"67009_CR57","first-page":"160","volume":"13","author":"PG Paulino","year":"2018","unstructured":"Paulino, P. G. et al. Molecular epidemiology of Babesia vogeli in dogs from the southeastern region of Rio de Janeiro, Brazil. Vet. Parasitol. Reg. Stud. Rep. 13, 160\u2013165 (2018).","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"67009_CR58","doi-asserted-by":"publisher","DOI":"10.1016\/j.parepi.2020.e00186","volume":"11","author":"SS Obeta","year":"2020","unstructured":"Obeta, S. S. et al. Prevalence of canine babesiosis and their risk factors among asymptomatic dogs in the federal capital territory, Abuja, Nigeria. Parasite Epidemiol. Control 11, e00186 (2020).","journal-title":"Parasite Epidemiol. Control"},{"issue":"1","key":"67009_CR59","first-page":"97","volume":"15","author":"M Khanmohammadi","year":"2021","unstructured":"Khanmohammadi, M., Zolfaghari-Emameh, R., Arshadi, M., Razmjou, E. & Karimi, P. Molecular identification and genotyping of Babesia canis in dogs from Meshkin Shahr County, Northwestern Iran. J. Arthropod. Borne Dis. 15(1), 97 (2021).","journal-title":"J. Arthropod. Borne Dis."},{"issue":"5","key":"67009_CR60","doi-asserted-by":"publisher","first-page":"1186","DOI":"10.1111\/j.1939-1676.2011.00779.x","volume":"25","author":"RJ Mellanby","year":"2011","unstructured":"Mellanby, R. J. et al. Breed and sex risk factors for canine Babesiosis in South Africa. J. Vet. Intern. Med. 25(5), 1186\u20131189 (2011).","journal-title":"J. Vet. Intern. Med."},{"issue":"1","key":"67009_CR61","first-page":"86","volume":"1","author":"L Daniel","year":"2016","unstructured":"Daniel, L., Kujul, N., Kemza, S. & Ibukunoluwa, M. Retrospective study of the risk factors associated with canine babesiosis diagnosed at Veterinary Clinic Federal College of Animal Health and Production Technology, Vom, North-central Nigeria, 1999\u20132006. Int. J. Sci. Appl. Res. 1(1), 86\u201393 (2016).","journal-title":"Int. J. Sci. Appl. Res."},{"issue":"1","key":"67009_CR62","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1186\/s13071-022-05348-x","volume":"15","author":"AA Hegab","year":"2022","unstructured":"Hegab, A. A. et al. Screening and phylogenetic characterization of tick-borne pathogens in a population of dogs and associated ticks in Egypt. Parasites Vectors 15(1), 222 (2022).","journal-title":"Parasites Vectors"},{"issue":"1","key":"67009_CR63","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1186\/s13071-021-04943-8","volume":"14","author":"A Selim","year":"2021","unstructured":"Selim, A., Almohammed, H., Abdelhady, A., Alouffi, A. & Alshammari, F. A. Molecular detection and risk factors for Anaplasma platys infection in dogs from Egypt. Parasites Vectors 14(1), 429 (2021).","journal-title":"Parasites Vectors"},{"issue":"4","key":"67009_CR64","doi-asserted-by":"publisher","first-page":"972","DOI":"10.1590\/S1517-83822009000400032","volume":"40","author":"B Corona","year":"2009","unstructured":"Corona, B., Machado, H., Rodr\u00edguez, M. & Mart\u00ednez, S. Characterization of recombinant MSP5 Anaplasma marginale Havana isolate. Braz. J. Microbiol. 40(4), 972\u2013979 (2009).","journal-title":"Braz. J. Microbiol."},{"issue":"7","key":"67009_CR65","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/S0169-4758(99)01469-6","volume":"15","author":"GH Palmer","year":"1999","unstructured":"Palmer, G. H., Rurangirwa, F. R., Kocan, K. M. & Brown, W. C. Molecular basis for vaccine development against the Ehrlichial pathogen Anaplasma marginale. Parasitol. Today 15(7), 281\u2013286 (1999).","journal-title":"Parasitol. Today"},{"issue":"1","key":"67009_CR66","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1079\/AHR2005104","volume":"6","author":"J De Le Fuente","year":"2005","unstructured":"De Le Fuente, J. et al. Genetic diversity of anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development. Anim. Health Res. Rev. 6(1), 75\u201389 (2005).","journal-title":"Anim. Health Res. Rev."},{"issue":"4","key":"67009_CR67","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.vetpar.2003.11.015","volume":"119","author":"A Criado-Fornelio","year":"2004","unstructured":"Criado-Fornelio, A., G\u00f3nzalez-del-R\u0131o, M., Buling-Sara\u00f1a, A. & Barba-Carretero, J. The, \u201cexpanding universe\u201d of piroplasms. J. Vet. Parasitol. 119(4), 337\u2013345 (2004).","journal-title":"J. Vet. Parasitol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-67009-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-67009-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-67009-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T05:03:57Z","timestamp":1721711037000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-67009-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,23]]},"references-count":67,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["67009"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-67009-6","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,23]]},"assertion":[{"value":"6 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 July 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 July 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"16888"}}