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Different strains of\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    are capable of a wide range of regulatory manipulations in their diverse hosts, including the modulation of host cellular differentiation to influence host reproduction. The genetic basis for the majority of these phenotypes is unknown. The\n                    <jats:italic>w<\/jats:italic>\n                    Wil strain from the neotropical fruit fly,\n                    <jats:italic>Drosophila willistoni<\/jats:italic>\n                    , exhibits a remarkably high affinity for host germline-derived cells relative to the somatic cells. This trait could be leveraged for understanding how\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    influences the host germline and for controlling host populations in the field. To further the use of this strain in biological and biomedical research, we sequenced the genome of the\n                    <jats:italic>w<\/jats:italic>\n                    Wil strain isolated from host cell culture cells. Here, we present the first high quality Nanopore assembly of\n                    <jats:italic>w<\/jats:italic>\n                    Wil, the\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    endosymbiont of\n                    <jats:italic>D. willistoni<\/jats:italic>\n                    . Our assembly resulted in a circular genome of 1.27\u00a0Mb with a BUSCO completeness score of 99.7%. Consistent with other insect-associated\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    strains, comparative genomic analysis revealed that\n                    <jats:italic>w<\/jats:italic>\n                    Wil has a highly mosaic genome relative to the closely related\n                    <jats:italic>w<\/jats:italic>\n                    Mel and\n                    <jats:italic>w<\/jats:italic>\n                    Au strains from\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    and\n                    <jats:italic>Drosophila simulans<\/jats:italic>\n                    , respectively.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-68716-w","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:15:48Z","timestamp":1722471348000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Complete de novo assembly of Wolbachia endosymbiont of Drosophila willistoni using long-read genome sequencing"],"prefix":"10.1038","volume":"14","author":[{"given":"Jodie","family":"Jacobs","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Nakamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mira","family":"Mastoras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailey","family":"Loucks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cade","family":"Mirchandani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily","family":"Karim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Penunuri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciara","family":"Wanket","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelbi L.","family":"Russell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,8,1]]},"reference":[{"key":"68716_CR1","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1007\/978-3-030-51849-3_5","volume":"69","author":"SL Russell","year":"2020","unstructured":"Russell, S. 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