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House Dust Mite and storage mites were separated from the dust. The microbiome and resistome of mites and originating dust were assessed using a culture-independent approach. The bacterial microbiome of mites and dust were predominantly populated by Staphylococci. There was a highly significant (<jats:italic>P<\/jats:italic>\u2009=\u20090.005; Spearman\u2019s rank test) correlation between the bacterial microbiome of mites and the dust. One-hundred and eighteen antimicrobial resistance genes (ARGs) were associated with mites and 176 with dust. Both contained ARGs encoding resistance for multi drug resistances, macrolide-lincosamide-streptogramin B, mobile genetic elements, Beta-lactam, Tetracycline and Aminoglycosides. By contrast, 15 ARGs were found for a laboratory-grown strain of <jats:italic>Dermatophagoides pteronyssinus<\/jats:italic>. A significant difference (<jats:italic>P<\/jats:italic>\u2009=\u20090.03; t test) was found in means between the resistome of mites and the household dust from which they emanated. No significant correlations (<jats:italic>P<\/jats:italic>\u2009=\u20090.23 and <jats:italic>P<\/jats:italic>\u2009=\u20090.22; Mantel test) were observed between the microbiome and resistome of mite and dust samples. There was not a significant difference (<jats:italic>P<\/jats:italic>\u2009=\u20090.54; t-test) between the means of ARGs for homes with and without a history of antibiotic use.<\/jats:p>","DOI":"10.1038\/s41598-024-70686-y","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T17:32:15Z","timestamp":1724434335000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The bacterial microbiome and resistome of house dust mites in Irish homes"],"prefix":"10.1038","volume":"14","author":[{"given":"Amal","family":"Aljohani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Byrne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Fleming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"70686_CR1","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/j.ijheh.2005.03.003","volume":"208","author":"S Brasche","year":"2005","unstructured":"Brasche, S. & Bischof, W. 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