{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:30:23Z","timestamp":1790109023727,"version":"4.0.1"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"S\u00e3o Paulo Research Foundation\/FAPESP","award":["2018\/17707-3"],"award-info":[{"award-number":["2018\/17707-3"]}]},{"name":"S\u00e3o Paulo Research Foundation\/FAPESP","award":["2019\/01676-4"],"award-info":[{"award-number":["2019\/01676-4"]}]},{"name":"Indiana University School of Dentistry"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-70943-0","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T03:03:08Z","timestamp":1725505388000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Exploring the efficacy of in-vitro low-temperature plasma treatment on single and multispecies dental cariogenic biofilms"],"prefix":"10.1038","volume":"14","author":[{"given":"Leandro Wagner","family":"Figueira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Panariello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane Y.","family":"Koga-Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"70943_CR1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/s12903-018-0698-9","volume":"19","author":"N Zhou","year":"2019","unstructured":"Zhou, N. et al. Dental caries and associated factors in 3 to 5-year-old children in Zhejiang Province, China: An epidemiological survey. BMC Oral Health 19, 9 (2019).","journal-title":"BMC Oral Health"},{"key":"70943_CR2","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1177\/0022034514557546","volume":"94","author":"F Schwendicke","year":"2015","unstructured":"Schwendicke, F. et al. Socioeconomic inequality and caries: A systematic review and meta-analysis. J. Dent. Res. 94, 10\u201318 (2015).","journal-title":"J. Dent. Res."},{"key":"70943_CR3","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1038\/s41415-021-3775-4","volume":"231","author":"N Pitts","year":"2021","unstructured":"Pitts, N. et al. Understanding dental caries as a non-communicable disease. Br. Dent. J. 231, 749\u2013753 (2021).","journal-title":"Br. Dent. J."},{"key":"70943_CR4","doi-asserted-by":"crossref","unstructured":"- Rusu, L., Roi, A., Roi, C., Tigmeanu, C.V. & Ardelean, L.C. The Influence of Salivary pH on the Prevalence of Dental Caries (ed. Intech open) 108 (2022).","DOI":"10.5772\/intechopen.106154"},{"key":"70943_CR5","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.jcma.2017.07.012","volume":"81","author":"M Jamal","year":"2018","unstructured":"Jamal, M. et al. Bacterial biofilm and associated infections. J Chin. Med. Assoc. 81, 7\u201311 (2018).","journal-title":"J Chin. Med. Assoc."},{"key":"70943_CR6","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1177\/0022034520909313","volume":"99","author":"D Kim","year":"2020","unstructured":"Kim, D. & Koo, H. Spatial design of polymicrobial oral biofilm in its native disease state. J. Dent. Res. 99, 597\u2013603 (2020).","journal-title":"J. Dent. Res."},{"key":"70943_CR7","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1016\/j.actbio.2020.08.032","volume":"115","author":"KR Sims Jr","year":"2020","unstructured":"Sims, K. R. Jr. et al. Dual antibacterial drug-loaded nanoparticles synergistically improve treatment of Streptococcus mutans biofilms. Acta Biomater. 115, 418\u2013431 (2020).","journal-title":"Acta Biomater."},{"key":"70943_CR8","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/j.msec.2015.05.036","volume":"55","author":"MA Perez-Diaz","year":"2015","unstructured":"Perez-Diaz, M. A. et al. Silver nanoparticles with antimicrobial activities against Streptococcus mutans and their cytotoxic effect. Mater. Sci. Eng. C Mater. Biol. Appl. 55, 360\u2013366 (2015).","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"issue":"64","key":"70943_CR9","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1111\/j.1600-0757.2012.00453.x","volume":"2000","author":"RJJR Palmer","year":"2014","unstructured":"Palmer, R. J. J. R. Composition and development of oral bacterial communities. Periodontology 2000(64), 20\u201339 (2014).","journal-title":"Periodontology"},{"key":"70943_CR10","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1177\/0022034520979926","volume":"100","author":"A Grier","year":"2021","unstructured":"Grier, A. et al. Oral microbiota composition predicts early childhood caries onset. J. Dent. Res. 100, 599\u2013607 (2021).","journal-title":"J. Dent. Res."},{"key":"70943_CR11","first-page":"325","volume":"38","author":"BL Edelstein","year":"2016","unstructured":"Edelstein, B. L., Ureles, S. D. & Smaldone, A. Very high salivary Streptococcus mutans predicts caries progression in young children. Pediatr. Dent. 38, 325\u2013330 (2016).","journal-title":"Pediatr. Dent."},{"key":"70943_CR12","doi-asserted-by":"publisher","first-page":"1795","DOI":"10.1007\/s00253-021-11140-1","volume":"105","author":"Y Cao","year":"2021","unstructured":"Cao, Y. & Lin, H. Characterization and function of membrane vesicles in gram-positive bacteria. Appl. Microbiol. Biotechnol. 105, 1795\u20131801 (2021).","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"70943_CR13","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1177\/0022034517731758","volume":"97","author":"L Eriksson","year":"2018","unstructured":"Eriksson, L. et al. Microbial complexes and caries in 17-Year-Olds with and without streptococcus mutans. J. Dent. Res. 97, 275\u2013282 (2018).","journal-title":"J. Dent. Res."},{"key":"70943_CR14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047722","volume":"7","author":"EL Gross","year":"2012","unstructured":"Gross, E. L. et al. Beyond Streptococcus mutans: Dental caries onset linked to multiple species by 16S rRNA community analysis. PLoS One. 7, e47722 (2012).","journal-title":"PLoS One."},{"key":"70943_CR15","doi-asserted-by":"publisher","first-page":"30170","DOI":"10.3402\/jom.v8.30170","volume":"8","author":"D Belstr\u00f8m","year":"2016","unstructured":"Belstr\u00f8m, D. et al. Salivary bacterial fingerprints of established oral disease revealed by the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) technique. J. Oral Microbiol. 8, 30170 (2016).","journal-title":"J. Oral Microbiol."},{"key":"70943_CR16","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1128\/IAI.00106-17","volume":"85","author":"VP Richards","year":"2017","unstructured":"Richards, V. P. et al. Microbiomes of site-specific dental plaques from children with different caries status. Infect. Immun. 85, 8 (2017).","journal-title":"Infect. Immun."},{"key":"70943_CR17","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.940602","volume":"12","author":"R Wu","year":"2022","unstructured":"Wu, R., Cui, G., Cao, Y., Zhao, W. & Lin, H. Streptococcus Mutans membrane vesicles enhance Candida albicans pathogenicity and carbohydrate metabolism. Front. Cell Infect. Microbiol. 12, 940602 (2022).","journal-title":"Front. Cell Infect. Microbiol."},{"key":"70943_CR18","doi-asserted-by":"publisher","first-page":"6357","DOI":"10.1128\/AEM.05203-11","volume":"77","author":"S Gregoire","year":"2011","unstructured":"Gregoire, S. et al. Role of glucosyltransferase B in interactions of Candida albicans with Streptococcus mutans and with an experimental pellicle on hydroxyapatite surfaces. Appl. Environ. Microbiol. 77, 6357\u20136367 (2011).","journal-title":"Appl. Environ. Microbiol."},{"key":"70943_CR19","doi-asserted-by":"publisher","first-page":"1879497m","DOI":"10.1080\/20002297.2021.1879497","volume":"13","author":"NH Thanh","year":"2021","unstructured":"Thanh, N. H. et al. Candida albicans Bgl2p, Ecm33p, and Als1p proteins are involved in adhesion to saliva-coated hydroxyapatite. J. Oral Microbiol. 13, 1879497m (2021).","journal-title":"J. Oral Microbiol."},{"key":"70943_CR20","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1177\/00220345211012385","volume":"101","author":"N Alkhars","year":"2022","unstructured":"Alkhars, N. et al. Oral candida predicts Streptococcus Mutans emergence in underserved US infants. J. Dent. Res. 101, 54\u201362 (2022).","journal-title":"J. Dent. Res."},{"key":"70943_CR21","doi-asserted-by":"publisher","first-page":"38","DOI":"10.2174\/1874285800802010038","volume":"2","author":"C Badet","year":"2008","unstructured":"Badet, C. & Thebaud, N. B. Ecology of lactobacilli in the oral cavity: A review of literature. Open Microbiol. J. 2, 38\u201348 (2008).","journal-title":"Open Microbiol. J."},{"key":"70943_CR22","doi-asserted-by":"crossref","unstructured":"Wen, Z.T., Huang, X., Ellepola, K., Liao, S., Li, Y. Lactobacilli and human dental caries: more than mechanical retention. Microbiology. 168, (2022).","DOI":"10.1099\/mic.0.001196"},{"issue":"Suppl","key":"70943_CR23","doi-asserted-by":"publisher","first-page":"110S","DOI":"10.1177\/0022034515576052","volume":"94","author":"PW Caufield","year":"2015","unstructured":"Caufield, P. W., Sch\u00f6n, C. N., Saraithong, P., Li, Y. & Argim\u00f3n, S. Oral Lactobacilli, and dental caries: A model for niche adaptation in humans. J Dent Res. 94(Suppl), 110S-S118 (2015).","journal-title":"J Dent Res."},{"key":"70943_CR24","doi-asserted-by":"publisher","DOI":"10.3389\/froh.2022.940177","volume":"7","author":"SS Gao","year":"2022","unstructured":"Gao, S. S., Du, M. & Maharani, D. A. Editorial: Minimally invasive dentistry for caries management. Front. Oral Health. 7, 940177 (2022).","journal-title":"Front. Oral Health."},{"key":"70943_CR25","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1038\/s41415-020-2153-y","volume":"229","author":"E Eden","year":"2022","unstructured":"Eden, E., Frencken, J., Gao, S., Horst, J. A. & Innes, N. Managing dental caries against the backdrop of COVID-19: Approaches to reduce aerosol generation. Br. Dent. J. 229, 411\u2013416 (2022).","journal-title":"Br. Dent. J."},{"key":"70943_CR26","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1007\/s40368-022-00746-2","volume":"23","author":"A BaniHani","year":"2022","unstructured":"BaniHani, A., Hamid, A., Van Eeckhoven, J., Gizani, S. & Albadri, S. Minimal Intervention Dentistry (MID) mainstream or unconventional option? Study exploring the impact of COVID-19 on paediatric dentists\u2019 views and practices of MID for managing carious primary teeth in children across the United Kingdom and European Union. Eur. Arch. Paediatr. Dent. 23, 835\u2013844 (2022).","journal-title":"Eur. Arch. Paediatr. Dent."},{"key":"70943_CR27","doi-asserted-by":"publisher","first-page":"211","DOI":"10.3390\/antibiotics10020211","volume":"10","author":"G Jungbauer","year":"2021","unstructured":"Jungbauer, G. et al. The antimicrobial effect of cold atmospheric plasma against dental pathogens\u2014A systematic review of in-vitro studies. Antibiotics (Basel). 10, 211 (2021).","journal-title":"Antibiotics (Basel)."},{"key":"70943_CR28","doi-asserted-by":"publisher","first-page":"570","DOI":"10.3390\/app11020570","volume":"11","author":"LW Figueira","year":"2021","unstructured":"Figueira, L. W., Panariello, B. H. D., Koga-Ito, C. Y. & Duarte, S. Low-temperature plasma as an approach for inhibiting a multispecies cariogenic biofilm. Appl. Sci. 11, 570 (2021).","journal-title":"Appl. Sci."},{"key":"70943_CR29","doi-asserted-by":"publisher","first-page":"23800","DOI":"10.1038\/s41598-021-03192-0","volume":"11","author":"G Nima","year":"2021","unstructured":"Nima, G. et al. Antibacterial efficacy of non-thermal atmospheric plasma against\u00a0Streptococcus mutans\u00a0biofilm grown on the surfaces of restorative resin composites. Sci. Rep. 11, 23800 (2021).","journal-title":"Sci. Rep."},{"key":"70943_CR30","doi-asserted-by":"publisher","first-page":"47","DOI":"10.3390\/plasma1010005","volume":"1","author":"M Laroussi","year":"2018","unstructured":"Laroussi, M. Plasma medicine: A brief introduction. Plasma. 1, 47\u201360 (2018).","journal-title":"Plasma."},{"key":"70943_CR31","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1111\/jam.13429","volume":"123","author":"P Bourke","year":"2017","unstructured":"Bourke, P., Ziuzina, D., Han, L., Cullen, P. & Gilmore, B. F. Microbiological interactions with cold plasma. J. Appl. Microbiol. 123, 308\u2013324 (2017).","journal-title":"J. Appl. Microbiol."},{"key":"70943_CR32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/ppap.201600145","volume":"14","author":"EC Neyts","year":"2017","unstructured":"Neyts, E. C. & Brault, P. Molecular dynamics simulations for plasma-surface interactions. Plasma Process. Polym. 14, 1\u20132 (2017).","journal-title":"Plasma Process. Polym."},{"key":"70943_CR33","doi-asserted-by":"publisher","first-page":"1737","DOI":"10.3390\/cancers13071737","volume":"13","author":"H Tanaka","year":"2021","unstructured":"Tanaka, H. et al. Plasma-treated solutions (PTS) in cancer therapy. Cancers. 13, 1737 (2021).","journal-title":"Cancers."},{"key":"70943_CR34","doi-asserted-by":"publisher","first-page":"1920","DOI":"10.3390\/cancers11121920","volume":"11","author":"A Privat-Maldonado","year":"2019","unstructured":"Privat-Maldonado, A., Bengtson, C., Razzokov, J., Smits, E. & Bogaerts, A. Modifying the tumour tmicroenvironment: Challenges and future perspectives for anticancer plasma treatments. Cancers. 11, 1920 (2019).","journal-title":"Cancers."},{"key":"70943_CR35","doi-asserted-by":"crossref","unstructured":"Arthur, R. A., Waeiss, R. A., Hara, A. T., Lippert, F., Eckert, G. J., Zero, D. T. Caries Res. 47, 318\u2013324 (2013).","DOI":"10.1159\/000347050"},{"key":"70943_CR36","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.cpme.2017.06.002","volume":"7","author":"AC Borges","year":"2017","unstructured":"Borges, A. C. et al. Cold atmospheric pressure plasma jet modulates\u00a0Candida albicans\u00a0virulence traits. Clin. Plasma Med. 7, 9\u201315 (2017).","journal-title":"Clin. Plasma Med."},{"key":"70943_CR37","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1615\/PlasmaMed.2014012361","volume":"4","author":"JA Delben","year":"2014","unstructured":"Delben, J. A. et al. Low-temperature plasma: An effective approach against Candida albicans biofilm. Plasma Med. 4, 231\u2013244 (2014).","journal-title":"Plasma Med."},{"key":"70943_CR38","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2021.108772","volume":"30","author":"F Ebrahimi-Shaghaghi","year":"2021","unstructured":"Ebrahimi-Shaghaghi, F., Noormohammadi, Z., Atyabi, S. M. & Razzaghi-Abyaneh, M. Inhibitory effects of cold atmospheric plasma on the growth, virulence factors and HSP90 gene expression in Candida albicans. Arch Biochem. Biophys. 30, 108772 (2021).","journal-title":"Arch Biochem. Biophys."},{"key":"70943_CR39","first-page":"84","volume":"27","author":"A Blumhagen","year":"2014","unstructured":"Blumhagen, A. et al. Plasma deactivation of oral bacteria seeded on hydroxyapatite disks as tooth enamel analogue. Am. J. Dent. 27, 84\u201390 (2014).","journal-title":"Am. J. Dent."},{"key":"70943_CR40","first-page":"07LG02","volume":"55","author":"H Qing","year":"2016","unstructured":"Qing, H. et al. Disinfection of\u00a0Streptococcus mutans\u00a0biofilm by a non-thermal atmospheric plasma brush. Appl. Phys. 55, 07LG02 (2016).","journal-title":"Appl. Phys."},{"key":"70943_CR41","doi-asserted-by":"publisher","first-page":"01B101","DOI":"10.1116\/1.4986208","volume":"1","author":"Y Duan","year":"2018","unstructured":"Duan, Y., Rani, S., Newberg, J. T. & Teplyakov, A. V. Investigation of the influence of oxygen plasma on supported silver nanoparticles. J. Vac. Sci. Technol. A. 1, 01B101 (2018).","journal-title":"J. Vac. Sci. Technol. A."},{"key":"70943_CR42","doi-asserted-by":"publisher","first-page":"23800","DOI":"10.1038\/s41598-021-03192-0","volume":"11","author":"G Nima","year":"2021","unstructured":"Nima, G. et al. Antibacterial efficacy of non-thermal atmospheric plasma against Streptococcus mutans biofilm grown on the surfaces of restorative resin composites. Sci. Rep. 11, 23800 (2021).","journal-title":"Sci. Rep."},{"key":"70943_CR43","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.jdent.2010.10.002","volume":"39","author":"B Yang","year":"2011","unstructured":"Yang, B. et al. Oral bacterial deactivation using a low-temperature atmospheric argon plasma brush. J. Dent. 39, 48\u201356 (2011).","journal-title":"J. Dent."},{"key":"70943_CR44","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1111\/idh.12615","volume":"21","author":"DG Kamath","year":"2023","unstructured":"Kamath, D. G., Nadimpalli, H., Nayak, S. U., Rajendran, V. & Natarajan, S. Comparison of antiplaque and anti-gingivitis effects of aloe vera mouthwash with chlorhexidine in fixed orthodontic patients\u2014A randomized controlled trial. Int. J. Dent. Hyg. 21, 211\u2013218 (2023).","journal-title":"Int. J. Dent. Hyg."},{"key":"70943_CR45","doi-asserted-by":"publisher","first-page":"3066","DOI":"10.1038\/s41598-020-59652-6","volume":"10","author":"MJ Nicol","year":"2020","unstructured":"Nicol, M. J. et al. Antibacterial effects of low-temperature plasma generated by atmospheric-pressure plasma jet are mediated by reactive oxygen species. Sci. Rep. 10, 3066 (2020).","journal-title":"Sci. Rep."},{"key":"70943_CR46","doi-asserted-by":"publisher","DOI":"10.1088\/1361-6463\/ac5e1c","volume":"55","author":"I Adamovich","year":"2022","unstructured":"Adamovich, I. et al. The 2022 plasma roadmap: Low temperature plasma science and technology. J. Phys. D Appl. Phys. 55, 373001 (2022).","journal-title":"J. Phys. D Appl. Phys."},{"key":"70943_CR47","doi-asserted-by":"publisher","first-page":"4519","DOI":"10.3390\/molecules27144519","volume":"27","author":"S Bekeschus","year":"2022","unstructured":"Bekeschus, S., Miebach, L., Pommerening, J., Clemen, R. & Witzke, K. Biological risk assessment of three dental composite materials following gas plasma exposure. Molecules. 27, 4519 (2022).","journal-title":"Molecules."},{"key":"70943_CR48","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1016\/j.tibtech.2017.07.013","volume":"36","author":"Z Machala","year":"2017","unstructured":"Machala, Z. & Graves, D. B. Frugal biotech applications of low-temperature plasma. Trends Biotechnol. 36, 579\u2013581 (2017).","journal-title":"Trends Biotechnol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-70943-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-70943-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-70943-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T04:02:19Z","timestamp":1725508939000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-70943-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,5]]},"references-count":48,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["70943"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-70943-0","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4339437\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,5]]},"assertion":[{"value":"28 April 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 August 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"20678"}}