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Malaria caused by neglected\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    parasites is often underestimated due to the lack of rapid diagnostic tools that can accurately detect these species. While nucleic-acid amplification technologies stand out as the most sensitive methods for detecting and confirming\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species, their implementation in resource-constrained settings poses significant challenges. Here, we present a Pan\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    recombinase polymerase amplification lateral flow (RPA\u2013LF) assay, capable of detecting all six human infecting\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species in low resource settings. The Pan\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    RPA-LF assay successfully detected low density clinical infections with a preliminary limit of detection between 10\u2013100\u00a0fg\/\u00b5l for\n                    <jats:italic>P. falciparum.<\/jats:italic>\n                    When combined with crude nucleic acid extraction, the assay can serve as a point-of-need tool for molecular xenomonitoring. This utility was demonstrated by screening laboratory-reared\n                    <jats:italic>Anopheles stephensi<\/jats:italic>\n                    mosquitoes fed with\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    -infected blood, as well as field samples of\n                    <jats:italic>An. funestus<\/jats:italic>\n                    s.l. and\n                    <jats:italic>An. gambiae<\/jats:italic>\n                    s.l. collected from central Africa. Overall, our proof-of-concept Pan\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    diagnostic tool has the potential to be applied for clinical and xenomonitoring field surveillance, and after further evaluation, could become an essential tool to assist malaria control and elimination.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-71129-4","type":"journal-article","created":{"date-parts":[[2024,8,30]],"date-time":"2024-08-30T04:04:24Z","timestamp":1724990664000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Pan Plasmodium lateral flow recombinase polymerase amplification assay for monitoring malaria parasites in vectors and human populations"],"prefix":"10.1038","volume":"14","author":[{"given":"Matthew","family":"Higgins","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojca","family":"Kristan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma L.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louisa A.","family":"Messenger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamille G.","family":"Dombrowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leen N.","family":"Vanheer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debbie","family":"Nolder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Drakeley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Stone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Almahamoudou","family":"Mahamar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teun","family":"Bousema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Delves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janvier","family":"Bandibabone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9v\u00e9rin","family":"N\u2019Do","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chimanuka","family":"Bantuzeko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertin","family":"Zawadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Walker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin J.","family":"Sutherland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio R. 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