{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T17:21:10Z","timestamp":1775841670956,"version":"3.50.1"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T00:00:00Z","timestamp":1732233600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T00:00:00Z","timestamp":1732233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"University of Padjadjaran"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Vitamin D and calcium in follicular fluid play an important role in modulating steroidogenesis, folliculogenesis, and oocyte quality determination. Both collaborate to produce top-quality embryos (TQE) during in vitro fertilization (IVF). In this study, we compared free 25(OH)D3 and calcium levels in follicular fluid between TQE and non-TQE groups. This cross-sectional study included women who underwent IVF procedures at tertiary hospitals in Bandung, Indonesia. Ovarian follicular fluid was collected during the ovum pick-up procedure. The examination of 25(OH)D3 levels, vitamin\n                    <jats:sc>d<\/jats:sc>\n                    -binding protein, and calcium in the follicles was done using an enzyme-linked immunosorbent assay (ELISA). Free 25(OH)D3 levels were calculated using the Vermeulen formula. A total of 173 samples met the study criteria, including 86 subjects in the TQE group and 87 subjects in the non-TQE group. There was a significant difference in free 25(OH)D3 follicular fluid levels between the TQE and non-TQE groups (p\u2009=\u20090.017); however, there was no significant difference in calcium levels between the two groups (p\u2009=\u20090.805). We also found that there was a significant association between free 25(OH)D3 follicular fluid levels and embryo quality (OR 3.05, 95% CI 1.46\u20136.38; p-value\u2009=\u20090.002); however, there was no significant association between follicular fluid calcium and embryo quality [p\u2009=\u20090.144 and OR, 1.74 (95% CI 0.82\u20133.68)]. The results suggest that free 25(OH)D3 and calcium in the follicular fluid act independently during steroidogenesis, folliculogenesis, and fertilization.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-71769-6","type":"journal-article","created":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T16:07:52Z","timestamp":1732291672000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Free 25(OH)D3 levels in follicular ovarian fluid top-quality embryos are higher than non-top-quality embryos in the normoresponders group"],"prefix":"10.1038","volume":"14","author":[{"given":"Artha Falentin","family":"Putri Susilo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanom Husni","family":"Syam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hartanto","family":"Bayuaji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Rachmawati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binarwan","family":"Halim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wiryawan","family":"Permadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tono","family":"Djuwantono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,11,22]]},"reference":[{"key":"71769_CR1","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.clinbiochem.2018.03.012","volume":"62","author":"M Vander Borght","year":"2018","unstructured":"Vander Borght, M. & Wyns, C. Fertility and infertility: Definition and epidemiology. Clin. Biochem. 62, 2\u201310 (2018).","journal-title":"Clin. Biochem."},{"issue":"1","key":"71769_CR2","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1001\/jama.2021.4788","volume":"326","author":"SA Carson","year":"2021","unstructured":"Carson, S. A. & Kallen, A. N. Diagnosis and management of infertility: A review. JAMA 326(1), 65\u201376 (2021).","journal-title":"JAMA"},{"key":"71769_CR3","unstructured":"Basile, S. et al. Vitamin D and infertility: A narrative review."},{"key":"71769_CR4","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1007\/s10815-018-1179-4","volume":"35","author":"P Ciepiela","year":"2018","unstructured":"Ciepiela, P. et al. Vitamin D as a follicular marker of human oocyte quality and a serum marker of in vitro fertilization outcome. J. Assist. Reprod. Genet. 35, 1265\u20131276 (2018).","journal-title":"J. Assist. Reprod. Genet."},{"key":"71769_CR5","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1007\/s11154-017-9407-2","volume":"18","author":"G Muscogiuri","year":"2017","unstructured":"Muscogiuri, G. et al. Shedding new light on female fertility: The role of vitamin D. Rev. Endocr. Metab. Disord. 18, 273\u2013283 (2017).","journal-title":"Rev. Endocr. Metab. Disord."},{"issue":"4","key":"71769_CR6","doi-asserted-by":"publisher","first-page":"671","DOI":"10.5604\/12321966.1226865","volume":"23","author":"P Skowro\u0144ska","year":"2016","unstructured":"Skowro\u0144ska, P. et al. The role of vitamin D in reproductive dysfunction in women-a systematic review. Ann. Agric. Environ. Med. 23(4), 671\u2013676 (2016).","journal-title":"Ann. Agric. Environ. Med."},{"issue":"3","key":"71769_CR7","first-page":"221","volume":"20","author":"R Hosseinisadat","year":"2022","unstructured":"Hosseinisadat, R. et al. Assessment of the effect of serum and follicular fluid vitamin D and glucose on assisted reproductive technique outcome: A cross-sectional study. Int. J. Reprod. BioMed. 20(3), 221 (2022).","journal-title":"Int. J. Reprod. BioMed."},{"key":"71769_CR8","doi-asserted-by":"publisher","DOI":"10.1530\/EC-21-0444","author":"JK Ko","year":"2022","unstructured":"Ko, J. K. et al. 100 years of vitamin D: Effect of serum vitamin D level before ovarian stimulation on the cumulative live birth rate of women undergoing in vitro fertilization: A retrospective analysis. Endocr. Connect. https:\/\/doi.org\/10.1530\/EC-21-0444 (2022).","journal-title":"Endocr. Connect."},{"issue":"3","key":"71769_CR9","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1111\/j.1365-2265.2012.04434.x","volume":"77","author":"RL Thomson","year":"2012","unstructured":"Thomson, R. L., Spedding, S. & Buckley, J. D. Vitamin D in the aetiology and management of polycystic ovary syndrome. Clin. Endocrinol. 77(3), 343\u2013350 (2012).","journal-title":"Clin. Endocrinol."},{"issue":"3","key":"71769_CR10","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1038\/nrendo.2013.262","volume":"10","author":"MB Jensen","year":"2014","unstructured":"Jensen, M. B. Vitamin D and male reproduction. Nat. Rev. Endocrinol. 10(3), 175\u2013186 (2014).","journal-title":"Nat. Rev. Endocrinol."},{"issue":"5","key":"71769_CR11","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.1016\/j.fertnstert.2020.05.040","volume":"114","author":"M Cozzolino","year":"2020","unstructured":"Cozzolino, M. et al. How vitamin D level influences in vitro fertilization outcomes: Results of a systematic review and meta-analysis. Fertil. Steril. 114(5), 1014\u20131025 (2020).","journal-title":"Fertil. Steril."},{"issue":"18","key":"71769_CR12","doi-asserted-by":"publisher","first-page":"3712","DOI":"10.3390\/nu14183712","volume":"14","author":"A Grzeczka","year":"2022","unstructured":"Grzeczka, A. et al. Relevance of vitamin D and its deficiency for the ovarian follicle and the oocyte: An update. Nutrients 14(18), 3712 (2022).","journal-title":"Nutrients"},{"key":"71769_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-809965-0.00051-3","author":"RF Chun","year":"2018","unstructured":"Chun, R. F. & Nielson, C. M. Free vitamin D: Concepts, assays, outcomes, and prospects. Vitamin D https:\/\/doi.org\/10.1016\/B978-0-12-809965-0.00051-3 (2018).","journal-title":"Vitamin D"},{"issue":"4","key":"71769_CR14","doi-asserted-by":"publisher","first-page":"215","DOI":"10.32007\/jfacmedbagdad.604758","volume":"60","author":"ZM Alawad","year":"2018","unstructured":"Alawad, Z. M. Level of follicular fluid vitamin D and embryo quality in a sample of Iraqi women undergoing IVF. J. Fac. Med. Baghdad 60(4), 215\u2013221 (2018).","journal-title":"J. Fac. Med. Baghdad"},{"key":"71769_CR15","doi-asserted-by":"publisher","first-page":"609524","DOI":"10.3389\/fendo.2020.609524","volume":"11","author":"A Arnanz","year":"2021","unstructured":"Arnanz, A. et al. Vitamin D in follicular fluid correlates with the euploid status of blastocysts in a vitamin D deficient population. Front. Endocrinol. 11, 609524 (2021).","journal-title":"Front. Endocrinol."},{"issue":"8","key":"71769_CR16","first-page":"2502","volume":"22","author":"G Monastra","year":"2018","unstructured":"Monastra, G. et al. Vitamin D: A steroid hormone with progesterone-like activity. Eur. Rev. Med. Pharmacol. Sci. 22(8), 2502\u20132512 (2018).","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"issue":"11","key":"71769_CR17","doi-asserted-by":"publisher","first-page":"3321","DOI":"10.1093\/humrep\/des280","volume":"27","author":"B Rudick","year":"2012","unstructured":"Rudick, B. et al. Characterizing the influence of vitamin D levels on IVF outcomes. Hum. Reprod. 27(11), 3321\u20133327 (2012).","journal-title":"Hum. Reprod."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-71769-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-71769-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-71769-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T17:04:32Z","timestamp":1732295072000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-71769-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,22]]},"references-count":17,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["71769"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-71769-6","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4375279\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,22]]},"assertion":[{"value":"6 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 August 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The study was conducted in accordance with the Declaration of Helsinki, and approved by the Institutional Review Board (or Ethics Committee) of Ethics Committee Review Board of Hasan Sadikin General Hospital \u2013 Faculty of Medicine, Universitas Padjadjaran (protocol date LB.02.01\/X.6.5\/349\/2022 and date of approval 06 October 2022). All study participants provided written informed consent prior to engaging in any study-related procedures. All authors hereby declare that all patients were examined in accordance with the ethical standards specified in the 1964 Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and institutional review board statement"}}],"article-number":"29023"}}