{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:03:12Z","timestamp":1789520592224,"version":"build-2803163510"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"crossref","award":["NNF16OC0021948"],"award-info":[{"award-number":["NNF16OC0021948"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"crossref","award":["NNF19OC0055857"],"award-info":[{"award-number":["NNF19OC0055857"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008398","name":"VILLUM FONDEN","doi-asserted-by":"crossref","award":["19175"],"award-info":[{"award-number":["19175"]}],"id":[{"id":"10.13039\/100008398","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003554","name":"Lundbeck Foundation","doi-asserted-by":"publisher","award":["R303-2018-2968"],"award-info":[{"award-number":["R303-2018-2968"]}],"id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-72233-1","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T16:02:39Z","timestamp":1726156959000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A head-to-head comparison of polymer interaction with mucin from porcine stomach and bovine submaxillary glands"],"prefix":"10.1038","volume":"14","author":[{"given":"Mai Bay","family":"Stie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiana","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob Judas Kain","family":"Kirkensgaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pernille S\u00f8nderby","family":"Tuelung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne M\u00f8rck","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vito","family":"Foder\u00e0","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stine","family":"R\u00f8nholt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,12]]},"reference":[{"key":"72233_CR1","doi-asserted-by":"crossref","unstructured":"Mortensen, J. S., Stie, M. B., Harloff-Helleberg, S. & Nielsen, H. M. Overcoming the mucus barrier. In Organelle and Molecular Targeting. 355\u2013380 (2021).","DOI":"10.1201\/9781003092773-14"},{"key":"72233_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1163\/156856208X393464","volume":"20","author":"NA Peppas","year":"2009","unstructured":"Peppas, N. A., Thomas, J. B. & McGinty, J. Molecular aspects of mucoadhesive carrier development for drug delivery and improved absorption. J. Biomater. Sci. Polym. Ed. 20, 1\u201320 (2009).","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"72233_CR3","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.ejpb.2014.01.001","volume":"87","author":"M Boegh","year":"2014","unstructured":"Boegh, M., Baldursd\u00f3ttir, S. G., M\u00fcllertz, A. & Nielsen, H. M. Property profiling of biosimilar mucus in a novel mucus-containing in vitro model for assessment of intestinal drug absorption. Eur. J. Pharmaceut. Biopharmaceut. 87, 227\u2013235 (2014).","journal-title":"Eur. J. Pharmaceut. Biopharmaceut."},{"key":"72233_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2021.113845","volume":"178","author":"M Marczynski","year":"2021","unstructured":"Marczynski, M., Kimna, C. & Lieleg, O. Purified mucins in drug delivery research. Adv. Drug Deliv. Rev. 178, 113845 (2021).","journal-title":"Adv. Drug Deliv. Rev."},{"key":"72233_CR5","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.addr.2017.09.023","volume":"124","author":"R Bansil","year":"2018","unstructured":"Bansil, R. & Turner, B. S. The biology of mucus: Composition, synthesis and organization. Adv. Drug Deliv. Rev. 124, 3\u201315 (2018).","journal-title":"Adv. Drug Deliv. Rev."},{"key":"72233_CR6","doi-asserted-by":"publisher","first-page":"1600534","DOI":"10.1002\/mabi.201600534","volume":"17","author":"AR Mackie","year":"2017","unstructured":"Mackie, A. R. et al. Innovative methods and applications in mucoadhesion research. Macromol. Biosci. 17, 1600534 (2017).","journal-title":"Macromol. Biosci."},{"key":"72233_CR7","doi-asserted-by":"crossref","unstructured":"Turner, B. S., Bhaskar, K. R., Hadzopoulou-Cladaras, M. & LaMont, J. T. Cysteine-rich regions of pig gastric mucin contain von Willebrand factor and cystine knot domains at the carboxyl terminal. Biochim. Biophys. Acta (BBA) - Gene Struct. Exp. 1447, 77\u201392 (1999).","DOI":"10.1016\/S0167-4781(99)00099-8"},{"key":"72233_CR8","doi-asserted-by":"publisher","first-page":"1580","DOI":"10.1021\/bm0609691","volume":"8","author":"JP Celli","year":"2007","unstructured":"Celli, J. P. et al. Rheology of gastric mucin exhibits a pH-dependent sol\u2212gel transition. Biomacromolecules 8, 1580\u20131586 (2007).","journal-title":"Biomacromolecules"},{"key":"72233_CR9","doi-asserted-by":"crossref","unstructured":"Lillehoj, E. P., Kato, K., Lu, W. & Kim, K. C. Cellular and Molecular Biology of Airway Mucins. 139\u2013202 (2013).","DOI":"10.1016\/B978-0-12-407697-6.00004-0"},{"key":"72233_CR10","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1097\/ACI.0b013e32830e6b04","volume":"8","author":"F Mantelli","year":"2008","unstructured":"Mantelli, F. & Arg\u00fceso, P. Functions of ocular surface mucins in health and disease. Curr. Opin. Allergy Clin. Immunol. 8, 477\u2013483 (2008).","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"72233_CR11","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1186\/1471-2164-12-140","volume":"12","author":"PR Hoorens","year":"2011","unstructured":"Hoorens, P. R. et al. Genome wide analysis of the bovine mucin genes and their gastrointestinal transcription profile. BMC Genomics 12, 140 (2011).","journal-title":"BMC Genomics"},{"key":"72233_CR12","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1146\/annurev-biochem-011520-105053","volume":"89","author":"GC Hansson","year":"2020","unstructured":"Hansson, G. C. Mucins and the microbiome. Annu. Rev. Biochem. 89, 769\u2013793 (2020).","journal-title":"Annu. Rev. Biochem."},{"key":"72233_CR13","doi-asserted-by":"publisher","first-page":"10678","DOI":"10.1021\/acsami.7b19762","volume":"10","author":"F Wan","year":"2018","unstructured":"Wan, F. et al. Lipid shell-enveloped polymeric nanoparticles with high integrity of lipid shells improve mucus penetration and interaction with cystic fibrosis-related bacterial biofilms. ACS Appl. Mater. Interfaces 10, 10678\u201310687 (2018).","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"72233_CR14","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.ejpb.2017.09.016","volume":"121","author":"E Marxen","year":"2017","unstructured":"Marxen, E., Mosgaard, M. D., Pedersen, A. M. L. & Jacobsen, J. Mucin dispersions as a model for the oromucosal mucus layer in in vitro and ex vivo buccal permeability studies of small molecules. Eur. J. Pharmaceut. Biopharmaceut. 121, 121\u2013128 (2017).","journal-title":"Eur. J. Pharmaceut. Biopharmaceut."},{"key":"72233_CR15","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1016\/j.ijpharm.2013.10.047","volume":"461","author":"E Meng-Lund","year":"2014","unstructured":"Meng-Lund, E., Muff-Westergaard, C., Sander, C., Madelung, P. & Jacobsen, J. A mechanistic based approach for enhancing buccal mucoadhesion of chitosan. Int. J. Pharm. 461, 280\u2013285 (2014).","journal-title":"Int. J. Pharm."},{"key":"72233_CR16","doi-asserted-by":"publisher","first-page":"116428","DOI":"10.1016\/j.carbpol.2020.116428","volume":"242","author":"MB Stie","year":"2020","unstructured":"Stie, M. B. et al. Swelling of mucoadhesive electrospun chitosan\/polyethylene oxide nanofibers facilitates adhesion to the sublingual mucosa. Carbohydr. Polym. 242,\u00a0116428\u00a0(2020).","journal-title":"Carbohydr. Polym."},{"key":"72233_CR17","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3389\/fphys.2022.912049","volume":"13","author":"M V\u00f6ller","year":"2022","unstructured":"V\u00f6ller, M. et al. An optimized protocol for assessment of sputum macrorheology in health and muco-obstructive lung disease. Front. Physiol. 13, 19 (2022).","journal-title":"Front. Physiol."},{"key":"72233_CR18","doi-asserted-by":"crossref","unstructured":"Sandberg, T., Blom, H. & Caldwell, K. D. Potential use of mucins as biomaterial coatings. I. Fractionation, characterization, and model adsorption of bovine, porcine, and human mucins. J. Biomed. Mater. Res. A 91A, 762\u2013772 (2009).","DOI":"10.1002\/jbm.a.32266"},{"key":"72233_CR19","doi-asserted-by":"publisher","first-page":"32","DOI":"10.3390\/biomimetics4020032","volume":"4","author":"M Collado-Gonz\u00e1lez","year":"2019","unstructured":"Collado-Gonz\u00e1lez, M., Gonz\u00e1lez Espinosa, Y. & Goycoolea, F. M. Interaction between chitosan and mucin: Fundamentals and applications. Biomimetics 4, 32 (2019).","journal-title":"Biomimetics"},{"key":"72233_CR20","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1021\/la011303p","volume":"18","author":"NV Efremova","year":"2002","unstructured":"Efremova, N. V., Huang, Y., Peppas, N. A. & Leckband, D. E. Direct measurement of interactions between tethered poly(ethylene glycol) chains and adsorbed mucin layers. Langmuir 18, 836\u2013845 (2002).","journal-title":"Langmuir"},{"key":"72233_CR21","doi-asserted-by":"publisher","first-page":"9687","DOI":"10.1021\/acs.langmuir.6b02057","volume":"32","author":"JB Madsen","year":"2016","unstructured":"Madsen, J. B. et al. Structural and mechanical properties of thin films of bovine submaxillary mucin versus porcine gastric mucin on a hydrophobic surface in aqueous solutions. Langmuir 32, 9687\u20139696 (2016).","journal-title":"Langmuir"},{"key":"72233_CR22","doi-asserted-by":"publisher","first-page":"7539","DOI":"10.1021\/acs.jpcb.8b05496","volume":"122","author":"Y Znamenskaya Falk","year":"2018","unstructured":"Znamenskaya Falk, Y., Engblom, J., Pedersen, J. S., Arnebrant, T. & Kocherbitov, V. Effects of hydration on structure and phase behavior of pig gastric mucin elucidated by SAXS. J. Phys. Chem. B 122, 7539\u20137546 (2018).","journal-title":"J. Phys. Chem. B"},{"key":"72233_CR23","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1107\/S0021889895011605","volume":"29","author":"G Beaucage","year":"1996","unstructured":"Beaucage, G. Small-angle scattering from polymeric mass fractals of arbitrary mass-fractal dimension. J. Appl. Crystallogr. 29, 134\u2013146 (1996).","journal-title":"J. Appl. Crystallogr."},{"key":"72233_CR24","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.1021\/bm800276d","volume":"9","author":"IA Sogias","year":"2008","unstructured":"Sogias, I. A., Williams, A. C. & Khutoryanskiy, V. V. Why is chitosan mucoadhesive?. Biomacromolecules 9, 1837\u20131842 (2008).","journal-title":"Biomacromolecules"},{"key":"72233_CR25","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2020.111219","volume":"194","author":"B\u00c1 Szil\u00e1gyi","year":"2020","unstructured":"Szil\u00e1gyi, B. \u00c1., Mammadova, A., Gyarmati, B. & Szil\u00e1gyi, A. Mucoadhesive interactions between synthetic polyaspartamides and porcine gastric mucin on the colloid size scale. Colloids Surf. B Biointerfaces 194, 111219 (2020).","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"72233_CR26","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1016\/j.ijpharm.2007.12.001","volume":"354","author":"J Thongborisute","year":"2008","unstructured":"Thongborisute, J. & Takeuchi, H. Evaluation of mucoadhesiveness of polymers by BIACORE method and mucin-particle method. Int. J. Pharm. 354, 204\u2013209 (2008).","journal-title":"Int. J. Pharm."},{"key":"72233_CR27","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.colsurfb.2012.10.012","volume":"103","author":"T Klemetsrud","year":"2013","unstructured":"Klemetsrud, T., Jonassen, H., Hiorth, M., Kj\u00f8niksen, A.-L. & Smistad, G. Studies on pectin-coated liposomes and their interaction with mucin. Colloids Surf. B Biointerfaces 103, 158\u2013165 (2013).","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"72233_CR28","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.jcis.2009.03.061","volume":"336","author":"M Lundin","year":"2009","unstructured":"Lundin, M., Sandberg, T., Caldwell, K. D. & Blomberg, E. Comparison of the adsorption kinetics and surface arrangement of \u201cas received\u201d and purified bovine submaxillary gland mucin (BSM) on hydrophilic surfaces. J. Colloid Interface Sci. 336, 30\u201339 (2009).","journal-title":"J. Colloid Interface Sci."},{"key":"72233_CR29","doi-asserted-by":"publisher","first-page":"1546","DOI":"10.1002\/mabi.201500158","volume":"15","author":"YA Albarkah","year":"2015","unstructured":"Albarkah, Y. A., Green, R. J. & Khutoryanskiy, V. V. Probing the mucoadhesive interactions between porcine gastric mucin and some water-soluble polymers. Macromol. Biosci. 15, 1546\u20131553 (2015).","journal-title":"Macromol. Biosci."},{"key":"72233_CR30","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/j.ejpb.2015.08.002","volume":"96","author":"S Oh","year":"2015","unstructured":"Oh, S., Wilcox, M., Pearson, J. P. & Borr\u00f3s, S. Optimal design for studying mucoadhesive polymers interaction with gastric mucin using a quartz crystal microbalance with dissipation (QCM-D): Comparison of two different mucin origins. Eur. J. Pharmaceut. Biopharmaceut. 96, 477\u2013483 (2015).","journal-title":"Eur. J. Pharmaceut. Biopharmaceut."},{"key":"72233_CR31","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1166\/jbn.2014.1768","volume":"10","author":"L Mazzarino","year":"2014","unstructured":"Mazzarino, L., Coche-Gu\u00e9rente, L., Lemos-Senna, P. L. E. & Borsali, R. On the mucoadhesive properties of chitosan-coated polycaprolactone nanoparticles loaded with curcumin using quartz crystal microbalance with dissipation monitoring. J. Biomed. Nanotechnol. 10, 787\u2013794 (2014).","journal-title":"J. Biomed. Nanotechnol."},{"key":"72233_CR32","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1016\/j.jcis.2007.07.029","volume":"315","author":"AA Feiler","year":"2007","unstructured":"Feiler, A. A., Sahlholm, A., Sandberg, T. & Caldwell, K. D. Adsorption and viscoelastic properties of fractionated mucin (BSM) and bovine serum albumin (BSA) studied with quartz crystal microbalance (QCM-D). J. Colloid Interface Sci. 315, 475\u2013481 (2007).","journal-title":"J. Colloid Interface Sci."},{"key":"72233_CR33","doi-asserted-by":"publisher","first-page":"1545","DOI":"10.1107\/S1600576717011438","volume":"50","author":"JB Hopkins","year":"2017","unstructured":"Hopkins, J. B., Gillilan, R. E. & Skou, S. BioXTAS RAW: Improvements to a free open-source program for small-angle X-ray scattering data reduction and analysis. J. Appl. Crystallogr. 50, 1545\u20131553 (2017).","journal-title":"J. Appl. Crystallogr."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-72233-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-72233-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-72233-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T16:10:18Z","timestamp":1726157418000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-72233-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,12]]},"references-count":33,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["72233"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-72233-1","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,12]]},"assertion":[{"value":"31 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 September 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"21350"}}