{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:25:42Z","timestamp":1780413942028,"version":"3.54.1"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T00:00:00Z","timestamp":1726444800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T00:00:00Z","timestamp":1726444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RF1NS129567, R01NS100876"],"award-info":[{"award-number":["RF1NS129567, R01NS100876"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-72542-5","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T08:20:54Z","timestamp":1726647654000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Neuronal tissue collection from intra-cranial instruments used in deep brain stimulation surgery for Parkinson\u2019s disease with implications for study of alpha-synuclein"],"prefix":"10.1038","volume":"14","author":[{"given":"Zachary A.","family":"Sorrentino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Riklan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace M.","family":"Lloyd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon P.","family":"Lucke-Wold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Mampre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Quintin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasheedat","family":"Zakare-Fagbamila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Still","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vyshak","family":"Chandra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly D.","family":"Foote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit I.","family":"Giasson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin D.","family":"Hilliard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,16]]},"reference":[{"key":"72542_CR1","doi-asserted-by":"publisher","first-page":"10224","DOI":"10.1074\/jbc.REV120.011743","volume":"295","author":"ZA Sorrentino","year":"2020","unstructured":"Sorrentino, Z. A. & Giasson, B. I. The emerging role of \u03b1-synuclein truncation in aggregation and disease. J. Biol. Chem. 295, 10224\u201310244. https:\/\/doi.org\/10.1074\/jbc.REV120.011743 (2020).","journal-title":"J. Biol. Chem."},{"key":"72542_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00401-019-01977-2","volume":"138","author":"ZA Sorrentino","year":"2019","unstructured":"Sorrentino, Z. A., Giasson, B. I. & Chakrabarty, P. \u03b1-Synuclein and astrocytes: tracing the pathways from homeostasis to neurodegeneration in Lewy body disease. Acta Neuropathol. 138, 1\u201321. https:\/\/doi.org\/10.1007\/s00401-019-01977-2 (2019).","journal-title":"Acta Neuropathol."},{"key":"72542_CR3","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1016\/s0140-6736(23)01478-2","volume":"403","author":"HR Morris","year":"2024","unstructured":"Morris, H. R., Spillantini, M. G., Sue, C. M. & Williams-Gray, C. H. The pathogenesis of Parkinson\u2019s disease. Lancet (London, England) 403, 293\u2013304. https:\/\/doi.org\/10.1016\/s0140-6736(23)01478-2 (2024).","journal-title":"Lancet (London, England)"},{"key":"72542_CR4","doi-asserted-by":"publisher","first-page":"2284","DOI":"10.1016\/s0140-6736(21)00218-x","volume":"397","author":"BR Bloem","year":"2021","unstructured":"Bloem, B. R., Okun, M. S. & Klein, C. Parkinson\u2019s disease. Lancet (London, England) 397, 2284\u20132303. https:\/\/doi.org\/10.1016\/s0140-6736(21)00218-x (2021).","journal-title":"Lancet (London, England)"},{"key":"72542_CR5","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1111\/neup.12691","volume":"40","author":"K Wakabayashi","year":"2020","unstructured":"Wakabayashi, K. Where and how alpha-synuclein pathology spreads in Parkinson\u2019s disease. Neuropathology 40, 415\u2013425. https:\/\/doi.org\/10.1111\/neup.12691 (2020).","journal-title":"Neuropathology"},{"key":"72542_CR6","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1016\/s0140-6736(14)61393-3","volume":"386","author":"LV Kalia","year":"2015","unstructured":"Kalia, L. V. & Lang, A. E. Parkinson\u2019s disease. Lancet (London, England) 386, 896\u2013912. https:\/\/doi.org\/10.1016\/s0140-6736(14)61393-3 (2015).","journal-title":"Lancet (London, England)"},{"key":"72542_CR7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2022.120190","volume":"435","author":"V Chandra","year":"2022","unstructured":"Chandra, V., Hilliard, J. D. & Foote, K. D. Deep brain stimulation for the treatment of tremor. J. Neurol. Sci. 435, 120190. https:\/\/doi.org\/10.1016\/j.jns.2022.120190 (2022).","journal-title":"J. Neurol. Sci."},{"key":"72542_CR8","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1111\/ane.13101","volume":"139","author":"I Mavroudis","year":"2019","unstructured":"Mavroudis, I., Petridis, F. & Kazis, D. Neuroimaging and neuropathological findings in essential tremor. Acta Neurol. Scand. 139, 491\u2013496. https:\/\/doi.org\/10.1111\/ane.13101 (2019).","journal-title":"Acta Neurol. Scand."},{"key":"72542_CR9","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.nbd.2018.09.005","volume":"120","author":"ZA Sorrentino","year":"2018","unstructured":"Sorrentino, Z. A. et al. Motor neuron loss and neuroinflammation in a model of \u03b1-synuclein-induced neurodegeneration. Neurobiol. Dis. 120, 98\u2013106. https:\/\/doi.org\/10.1016\/j.nbd.2018.09.005 (2018).","journal-title":"Neurobiol. Dis."},{"key":"72542_CR10","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1084\/jem.20112457","volume":"209","author":"KC Luk","year":"2012","unstructured":"Luk, K. C. et al. Intracerebral inoculation of pathological alpha-synuclein initiates a rapidly progressive neurodegenerative alpha-synucleinopathy in mice. J. Exp. Med. 209, 975\u2013986. https:\/\/doi.org\/10.1084\/jem.20112457 (2012).","journal-title":"J. Exp. Med."},{"key":"72542_CR11","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1038\/s41374-019-0195-z","volume":"99","author":"RJ Karpowicz Jr","year":"2019","unstructured":"Karpowicz, R. J. Jr., Trojanowski, J. Q. & Lee, V. M. Transmission of \u03b1-synuclein seeds in neurodegenerative disease: Recent developments. Lab. Investig. 99, 971\u2013981. https:\/\/doi.org\/10.1038\/s41374-019-0195-z (2019).","journal-title":"Lab. Investig."},{"key":"72542_CR12","doi-asserted-by":"publisher","first-page":"18914","DOI":"10.1074\/jbc.RA118.005603","volume":"293","author":"ZA Sorrentino","year":"2018","unstructured":"Sorrentino, Z. A. et al. Physiological C-terminal truncation of \u03b1-synuclein potentiates the prion-like formation of pathological inclusions. J. Biol. Chem. 293, 18914\u201318932. https:\/\/doi.org\/10.1074\/jbc.RA118.005603 (2018).","journal-title":"J. Biol. Chem."},{"key":"72542_CR13","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/s13024-017-0182-z","volume":"12","author":"ZA Sorrentino","year":"2017","unstructured":"Sorrentino, Z. A. et al. Intrastriatal injection of alpha-synuclein can lead to widespread synucleinopathy independent of neuroanatomic connectivity. Mol. Neurodegener. 12, 40. https:\/\/doi.org\/10.1186\/s13024-017-0182-z (2017).","journal-title":"Mol. Neurodegener."},{"issue":"Suppl 1","key":"72542_CR14","doi-asserted-by":"publisher","first-page":"S85","DOI":"10.1016\/s1353-8020(13)70022-1","volume":"20","author":"KC Luk","year":"2014","unstructured":"Luk, K. C. & Lee, V. M. Modeling Lewy pathology propagation in Parkinson\u2019s disease. Parkinsonism Relat. Disord. 20(Suppl 1), S85-87. https:\/\/doi.org\/10.1016\/s1353-8020(13)70022-1 (2014).","journal-title":"Parkinsonism Relat. Disord."},{"key":"72542_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2020.135017","volume":"732","author":"ZA Sorrentino","year":"2020","unstructured":"Sorrentino, Z. A. et al. Carboxy-terminal truncation and phosphorylation of \u03b1-synuclein elongates survival in a prion-like seeding mouse model of synucleinopathy. Neurosci. Lett. 732, 135017. https:\/\/doi.org\/10.1016\/j.neulet.2020.135017 (2020).","journal-title":"Neurosci. Lett."},{"key":"72542_CR16","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1186\/s40478-021-01242-2","volume":"9","author":"EW Hass","year":"2021","unstructured":"Hass, E. W. et al. Disease-, region- and cell type specific diversity of \u03b1-synuclein carboxy terminal truncations in synucleinopathies. Acta Neuropathol. Commun. 9, 146. https:\/\/doi.org\/10.1186\/s40478-021-01242-2 (2021).","journal-title":"Acta Neuropathol. Commun."},{"key":"72542_CR17","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1186\/s40478-023-01623-9","volume":"11","author":"GM Lloyd","year":"2023","unstructured":"Lloyd, G. M. et al. Carboxyl truncation of \u03b1-synuclein occurs early and is influenced by human APOE genotype in transgenic mouse models of \u03b1-synuclein pathogenesis. Acta Neuropathol. Commun. 11, 119. https:\/\/doi.org\/10.1186\/s40478-023-01623-9 (2023).","journal-title":"Acta Neuropathol. Commun."},{"key":"72542_CR18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.104912","volume":"299","author":"S Quintin","year":"2023","unstructured":"Quintin, S. et al. Cellular processing of \u03b1-synuclein fibrils results in distinct physiological C-terminal truncations with a major cleavage site at residue Glu 114. J. Biol. Chem. 299, 104912. https:\/\/doi.org\/10.1016\/j.jbc.2023.104912 (2023).","journal-title":"J. Biol. Chem."},{"key":"72542_CR19","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1186\/s40478-022-01468-8","volume":"10","author":"MF Altay","year":"2022","unstructured":"Altay, M. F., Liu, A. K. L., Holton, J. L., Parkkinen, L. & Lashuel, H. A. Prominent astrocytic alpha-synuclein pathology with unique post-translational modification signatures unveiled across Lewy body disorders. Acta Neuropathol. Commun. 10, 163. https:\/\/doi.org\/10.1186\/s40478-022-01468-8 (2022).","journal-title":"Acta Neuropathol. Commun."},{"key":"72542_CR20","doi-asserted-by":"publisher","first-page":"9973","DOI":"10.1074\/jbc.RA119.008930","volume":"294","author":"RP McGlinchey","year":"2019","unstructured":"McGlinchey, R. P. et al. C-terminal \u03b1-synuclein truncations are linked to cysteine cathepsin activity in Parkinson\u2019s disease. J. Biol. Chem. 294, 9973\u20139984. https:\/\/doi.org\/10.1074\/jbc.RA119.008930 (2019).","journal-title":"J. Biol. Chem."},{"key":"72542_CR21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153785","volume":"11","author":"MA Higuchi","year":"2016","unstructured":"Higuchi, M. A. et al. Interdisciplinary Parkinson\u2019s disease deep brain stimulation screening and the relationship to unintended hospitalizations and quality of life. PLoS ONE 11, e0153785. https:\/\/doi.org\/10.1371\/journal.pone.0153785 (2016).","journal-title":"PLoS ONE"},{"key":"72542_CR22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145623","volume":"10","author":"MA Higuchi","year":"2015","unstructured":"Higuchi, M. A. et al. Impact of an interdisciplinary deep brain stimulation screening model on post-surgical complications in essential tremor patients. PLoS ONE 10, e0145623. https:\/\/doi.org\/10.1371\/journal.pone.0145623 (2015).","journal-title":"PLoS ONE"},{"key":"72542_CR23","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1007\/s40120-020-00220-5","volume":"10","author":"KLK Au","year":"2021","unstructured":"Au, K. L. K. et al. Globus pallidus internus (GPi) deep brain stimulation for parkinson\u2019s disease: Expert review and commentary. Neurol. Ther. 10, 7\u201330. https:\/\/doi.org\/10.1007\/s40120-020-00220-5 (2021).","journal-title":"Neurol. Ther."},{"key":"72542_CR24","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1212\/wnl.0000000000004058","volume":"89","author":"IG McKeith","year":"2017","unstructured":"McKeith, I. G. et al. Diagnosis and management of dementia with Lewy bodies: Fourth consensus report of the DLB Consortium. Neurology 89, 88\u2013100. https:\/\/doi.org\/10.1212\/wnl.0000000000004058 (2017).","journal-title":"Neurology"},{"key":"72542_CR25","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1002\/(sici)1097-4547(20000215)59:4<528::Aid-jnr8>3.0.Co;2-0","volume":"59","author":"BI Giasson","year":"2000","unstructured":"Giasson, B. I. et al. A panel of epitope-specific antibodies detects protein domains distributed throughout human alpha-synuclein in Lewy bodies of Parkinson\u2019s disease. J. Neurosci. Res. 59, 528\u2013533. https:\/\/doi.org\/10.1002\/(sici)1097-4547(20000215)59:4%3c528::Aid-jnr8%3e3.0.Co;2-0 (2000).","journal-title":"J. Neurosci. Res."},{"key":"72542_CR26","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1186\/s40478-019-0787-2","volume":"7","author":"ZA Sorrentino","year":"2019","unstructured":"Sorrentino, Z. A. et al. Unique \u03b1-synuclein pathology within the amygdala in Lewy body dementia: Implications for disease initiation and progression. Acta Neuropathol. Commun. 7, 142. https:\/\/doi.org\/10.1186\/s40478-019-0787-2 (2019).","journal-title":"Acta Neuropathol. Commun."},{"key":"72542_CR27","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1186\/s40478-022-01394-9","volume":"10","author":"G Paterno","year":"2022","unstructured":"Paterno, G., Bell, B. M., Gorion, K. M., Prokop, S. & Giasson, B. I. Reassessment of neuronal tau distribution in adult human brain and implications for tau pathobiology. Acta Neuropathol. Commun. 10, 94. https:\/\/doi.org\/10.1186\/s40478-022-01394-9 (2022).","journal-title":"Acta Neuropathol. Commun."},{"key":"72542_CR28","doi-asserted-by":"publisher","first-page":"29739","DOI":"10.1074\/jbc.M600933200","volume":"281","author":"JP Anderson","year":"2006","unstructured":"Anderson, J. P. et al. Phosphorylation of Ser-129 is the dominant pathological modification of alpha-synuclein in familial and sporadic Lewy body disease. J. Biol. Chem. 281, 29739\u201329752. https:\/\/doi.org\/10.1074\/jbc.M600933200 (2006).","journal-title":"J. Biol. Chem."},{"key":"72542_CR29","doi-asserted-by":"publisher","first-page":"39","DOI":"10.3233\/jpd-160779","volume":"6","author":"A Oueslati","year":"2016","unstructured":"Oueslati, A. Implication of alpha-synuclein phosphorylation at S129 in synucleinopathies: What have we learned in the last decade?. J. Parkinsons Dis. 6, 39\u201351. https:\/\/doi.org\/10.3233\/jpd-160779 (2016).","journal-title":"J. Parkinsons Dis."},{"key":"72542_CR30","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.3233\/jad-171013","volume":"61","author":"H Wang","year":"2018","unstructured":"Wang, H. et al. A longitudinal study of total and phosphorylated \u03b1-synuclein with other biomarkers in cerebrospinal fluid of Alzheimer\u2019s disease and mild cognitive impairment. J. Alzheimers Dis. 61, 1541\u20131553. https:\/\/doi.org\/10.3233\/jad-171013 (2018).","journal-title":"J. Alzheimers Dis."},{"key":"72542_CR31","doi-asserted-by":"publisher","first-page":"889","DOI":"10.3389\/fnins.2019.00889","volume":"13","author":"C Cariulo","year":"2019","unstructured":"Cariulo, C. et al. Phospho-S129 alpha-synuclein is present in human plasma but not in cerebrospinal fluid as determined by an ultrasensitive immunoassay. Front. Neurosci. 13, 889. https:\/\/doi.org\/10.3389\/fnins.2019.00889 (2019).","journal-title":"Front. Neurosci."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-72542-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-72542-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-72542-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T08:22:08Z","timestamp":1726647728000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-72542-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,16]]},"references-count":31,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["72542"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-72542-5","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4369598\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,16]]},"assertion":[{"value":"4 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 September 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"As described in the text, human research was IRB approved (IRB202300741) and proper consent processes followed with informed consent obtained from all participants.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}}],"article-number":"21641"}}