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The anti-cancer and antimicrobial properties were investigated. The obtained results revealed that total phenol (TP) and total flavonoid (TF) of total ethanol extract (100%) (FTE) were higher than 70% ethanol extract (SE), and these contents increased after gamma radiation with 5\u00a0kGy. The results of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the <jats:italic>Solanum aculeastrum<\/jats:italic> extracts suggested that FTE and 5\u00a0kGy-irradiated FTE can be used to control and prevent skin infections caused by MRSA and endocarditis, urinary tract infections, and prostatitis caused by <jats:italic>Enterococcus faecalis<\/jats:italic>. The FTE sample irradiated at 5\u00a0kGy showed cytotoxicity for A431 and Hct-116 cell lines similar to the control sample and higher than the toxicity revealed by the samples irradiated at 10\u00a0kGy. In normal cells (Bj-1), the toxicity was decreased after irradiation (IC50\u2009=\u200931 \u03bcg\/ml) compared to the non-irradiated extract (IC<jats:sub>50<\/jats:sub>\u2009=\u200926.1 \u03bcg\/ml). Molecular docking suggested Sortase A to play a role in chlorogenic acid antibacterial activity towards <jats:italic>Staphylococcus aureus<\/jats:italic>. In conclusion, \u03b3-irradiation can be used to enhance the phytoconstituents of <jats:italic>Solanum aculeastrum<\/jats:italic> fruit extracts and, consequently, its biological properties.<\/jats:p>","DOI":"10.1038\/s41598-024-73531-4","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T07:02:30Z","timestamp":1729580550000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Biochemical and biological studies of irradiated and non-irradiated extracts of Solanum aculeastrum Dunal fruit"],"prefix":"10.1038","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7208-3288","authenticated-orcid":false,"given":"Asmaa A.","family":"Amer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A. 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