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For SMFA, media with ~\u200910% of human serum has been used for infectious gametocyte cultures, however, there are multiple challenges to obtain a suitable human serum. Here we show a human-serum-free culture medium (HSF), which was a mixture of two stem cell culture media and AlbuMAX, supported infectious gametocyte growth. Moreover, the HSF-induced gametocytes elicited significantly higher numbers of oocysts compared to gametocytes cultured with conventional human serum medium (Conv). While some caution is required when comparing percent transmission reducing activity data generated from HSF-SMFA and Conv-SMFA, the HSF method can facilitate the establishment of gametocyte cultures or SMFA by bypassing the need for human serum. Thus, this study will support future development of <jats:italic>P. falciparum<\/jats:italic> transmission-blocking interventions.<\/jats:p>","DOI":"10.1038\/s41598-024-73843-5","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T14:04:17Z","timestamp":1727445857000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A human-serum-free medium can induce more infectious P. falciparum gametocytes than a conventional human-serum-containing medium"],"prefix":"10.1038","volume":"14","author":[{"given":"Kazutoyo","family":"Miura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingbing","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ragavan","family":"Varadharajan Suresh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonas T.","family":"Gebremicale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luwen","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thao P.","family":"Pham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle","family":"Roche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ababacar","family":"Diouf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan F.","family":"Lovell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Philippe","family":"Julien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carole A.","family":"Long","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,9,27]]},"reference":[{"key":"73843_CR1","unstructured":"World Health Organization. https:World Malaria Report (2023). https:\/\/www.who.int\/teams\/global-malaria-programme\/reports\/world-malaria-report-2023 (Accessed on May 17, 2024)."},{"key":"73843_CR2","doi-asserted-by":"publisher","first-page":"3","DOI":"10.4161\/hv.6.1.9604","volume":"6","author":"RE Sinden","year":"2010","unstructured":"Sinden, R. E. A biologist\u2019s perspective on malaria vaccine development. Hum. Vaccin. 6, 3\u201311 (2010).","journal-title":"Hum. Vaccin"},{"key":"73843_CR3","doi-asserted-by":"publisher","first-page":"1596","DOI":"10.1016\/S1473-3099(22)00428-5","volume":"22","author":"SC van der Boor","year":"2022","unstructured":"van der Boor, S. C. et al. Safety, tolerability, and Plasmodium Falciparum transmission-reducing activity of monoclonal antibody TB31F: a single-centre, open-label, first-in-human, dose-escalation, phase 1 trial in healthy malaria-naive adults. Lancet Infect. Dis. 22, 1596\u20131605. https:\/\/doi.org\/10.1016\/S1473-3099(22)00428-5 (2022).","journal-title":"Lancet Infect. Dis."},{"key":"73843_CR4","doi-asserted-by":"publisher","first-page":"15160","DOI":"10.1038\/ncomms15160","volume":"8","author":"C Miguel-Blanco","year":"2017","unstructured":"Miguel-Blanco, C. et al. Hundreds of dual-stage antimalarial molecules discovered by a functional gametocyte screen. Nat. Commun. 8, 15160. https:\/\/doi.org\/10.1038\/ncomms15160 (2017).","journal-title":"Nat. Commun."},{"key":"73843_CR5","doi-asserted-by":"publisher","first-page":"3805","DOI":"10.1038\/s41467-018-05777-2","volume":"9","author":"MJ Delves","year":"2018","unstructured":"Delves, M. J. et al. A high throughput screen for next-generation leads targeting malaria parasite transmission. Nat. Commun. 9, 3805. https:\/\/doi.org\/10.1038\/s41467-018-05777-2 (2018).","journal-title":"Nat. Commun."},{"key":"73843_CR6","doi-asserted-by":"publisher","first-page":"7764","DOI":"10.1038\/s41598-018-26125-w","volume":"8","author":"G Colmenarejo","year":"2018","unstructured":"Colmenarejo, G. et al. Predicting transmission blocking potential of anti-malarial compounds in the mosquito feeding assay using Plasmodium Falciparum male gamete inhibition assay. Sci. Rep. 8, 7764. https:\/\/doi.org\/10.1038\/s41598-018-26125-w (2018).","journal-title":"Sci. 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Vaccine. 34, 4145\u20134151. https:\/\/doi.org\/10.1016\/j.vaccine.2016.06.066 (2016).","journal-title":"Vaccine"},{"key":"73843_CR9","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1038\/294364a0","volume":"294","author":"T Ifediba","year":"1981","unstructured":"Ifediba, T. & Vanderberg, J. P. Complete in vitro maturation of Plasmodium Falciparum gametocytes. Nature. 294, 364\u2013366 (1981).","journal-title":"Nature"},{"key":"73843_CR10","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1016\/0035-9203(82)90116-X","volume":"76","author":"T Ponnudurai","year":"1982","unstructured":"Ponnudurai, T., Lensen, A. H., Leeuwenberg, A. D. & Meuwissen, J. H. Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures. Trans. R Soc. Trop. Med. Hyg. 76, 812\u2013818 (1982).","journal-title":"Trans. R Soc. Trop. Med. 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