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Hydrogel nanocomposites were prepared from acrylamide (Am), chitosan (CS) and AgNO\n                    <jats:sub>3<\/jats:sub>\n                    utilizing gamma rays. Diverse variables were applied in preparation of silver nanoparticles-laoded hydrogel nanocomposites of (PAm\/CS)-AgNPs such as influence of radiation dose and influnece of CS concentration. Diverse techniques were utilized to characterize hydrogel nanocomposites; Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) and scanning electron microscopy (SEM). Results confirmed formation of silver nanoparticles-loaded hydrogel nanocomposites of (PAm\/CS)-AgNPs. Antifungal activity of (PAm\/CS)-AgNPs hydrogel nanocomposites on viability of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    was esitmated. Results displayed the efficient microbial inhibition activity of treatment against\n                    <jats:italic>C. albicans<\/jats:italic>\n                    compared to control. Furthermore, (PAm\/CS)-AgNPs hydrogel nanocomposite against cervical cancer HeLa cell line was investigated. Cytotoxicity of (PAm\/CS)-AgNPs hydrogel nanocomposites on prior cancer cell line empolyed to prohibition of cell growth assesssed by MTT test. HeLa cancer cell is treated by (PAm\/CS)-AgNPs for 48\u00a0h exposed a potential apoptotic activity by noticeable up-regulation of p53 gene expression. Moreover, anticancer activity was investigated by down-regulation of platelet-based growth variable receptor beta (PDGFR-\u03b2), Bcl2, Cathepsine, and MMP-2 gene expression. antioxidant activity was investigated and results showed antioxidant activity of (PAm\/CS) hydrogel and (PAm\/CS)-AgNPs hydrogel nanocomposite are 87.8% and 62.9%, respectively.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-74027-x","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T05:20:06Z","timestamp":1730092806000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Gamma irradiation green synthesis of (polyacrylamide\/chitosan\/silver nanoparticles) hydrogel nanocomposites and their using as antifungal against Candida albicans and anti-cancer modulator"],"prefix":"10.1038","volume":"14","author":[{"given":"Salwa A.","family":"Khalil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Awadallah-F","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mervat R.","family":"Khaffaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha Mohammad","family":"Fathy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad S.","family":"Kodous","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,10,28]]},"reference":[{"key":"74027_CR1","doi-asserted-by":"crossref","unstructured":"Harris, B. 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