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Two variations of BimA in <jats:italic>B. pseudomallei<\/jats:italic> have been reported previously: BimA<jats:sub>Bp<\/jats:sub> and BimA <jats:italic>B. mallei<\/jats:italic>-like (BimA<jats:sub>Bm<\/jats:sub>). However, little is known about genetic sequence variations within BimA and BimC, and their potential effect on the virulence of <jats:italic>B. pseudomallei<\/jats:italic>. This study analyzed 1,294 genomes from clinical isolates of patients admitted to nine hospitals in northeast Thailand between 2015 and 2018 and performed 3D structural analysis and plaque-forming efficiency assay. The genomic analysis identified 10 BimA<jats:sub>Bp<\/jats:sub> and 5 major BimC types, in the dominant and non-dominant lineages of the <jats:italic>B. pseudomallei<\/jats:italic> population structure. Our protein prediction analysis of all BimA<jats:sub>Bp<\/jats:sub> and major BimC variants revealed that their 3D structures were conserved compared to those of <jats:italic>B. pseudomallei<\/jats:italic> K96243. Sixteen representative strains of the most distant BimA<jats:sub>Bp<\/jats:sub> types were tested for plaque formation and the development of polar actin tails in A549 epithelial cells. We found that all isolates retained these functions. These findings enhance our understanding of the prevalence of BimA<jats:sub>Bp<\/jats:sub> and BimC variants and their implications for <jats:italic>B. pseudomallei<\/jats:italic> virulence.<\/jats:p>","DOI":"10.1038\/s41598-024-74922-3","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T05:02:19Z","timestamp":1729659739000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Genetic variation, structural analysis, and virulence implications of BimA and BimC in clinical isolates of Burkholderia pseudomallei in Thailand"],"prefix":"10.1038","volume":"14","author":[{"given":"Charlene Mae Salao","family":"Cagape","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rathanin","family":"Seng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natnaree","family":"Saiprom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarunporn","family":"Tandhavanant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Chewapreecha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usa","family":"Boonyuen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. 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