{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T11:43:34Z","timestamp":1781523814766,"version":"3.54.1"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T00:00:00Z","timestamp":1728086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T00:00:00Z","timestamp":1728086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Self-financing Project of Harbin Science and Technology Plan","award":["2023ZCZJCG013"],"award-info":[{"award-number":["2023ZCZJCG013"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1302203"],"award-info":[{"award-number":["2022YFD1302203"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA0805902"],"award-info":[{"award-number":["2021YFA0805902"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372884"],"award-info":[{"award-number":["32372884"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-74969-2","type":"journal-article","created":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T02:01:52Z","timestamp":1728093712000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Melatonin decreases excessive polyspermy for single oocyte in pigs through the MT2 receptor"],"prefix":"10.1038","volume":"14","author":[{"given":"Jing-Tao","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Hui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang-Yu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren-Fei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Jia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Gang","family":"Weng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong-Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao-Xiu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Xue","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,10,5]]},"reference":[{"key":"74969_CR1","doi-asserted-by":"publisher","first-page":"1580","DOI":"10.1095\/biolreprod.103.018937","volume":"69","author":"YH Li","year":"2003","unstructured":"Li, Y. H. et al. Reduced polyspermic penetration in porcine oocytes inseminated in a new in vitro fertilization (IVF) system: straw IVF. Biol. Reprod. 69, 1580\u20131585. https:\/\/doi.org\/10.1095\/biolreprod.103.018937 (2003).","journal-title":"Biol. Reprod."},{"key":"74969_CR2","doi-asserted-by":"publisher","first-page":"e13401","DOI":"10.1111\/asj.13401","volume":"91","author":"HT Nguyen","year":"2020","unstructured":"Nguyen, H. T. et al. Selection based on morphological features of porcine embryos produced by in vitro fertilization: timing of early cleavages and the effect of polyspermy. Anim. Sci. J. 91, e13401. https:\/\/doi.org\/10.1111\/asj.13401 (2020).","journal-title":"Anim. Sci. J."},{"key":"74969_CR3","doi-asserted-by":"publisher","first-page":"1892","DOI":"10.1093\/oxfordjournals.humrep.a137955","volume":"8","author":"H Balakier","year":"1993","unstructured":"Balakier, H. Tripronuclear human zygotes: the first cell cycle and subsequent development. Hum. Reprod. 8, 1892\u20131897. https:\/\/doi.org\/10.1093\/oxfordjournals.humrep.a137955 (1993).","journal-title":"Hum. Reprod."},{"key":"74969_CR4","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1111\/asj.12327","volume":"86","author":"H Kitaji","year":"2015","unstructured":"Kitaji, H., Ookutsu, S., Sato, M. & Miyoshi, K. A new rolling culture-based in vitro fertilization system capable of reducing polyspermy in porcine oocytes. Anim. Sci. J. 86, 494\u2013498. https:\/\/doi.org\/10.1111\/asj.12327 (2015).","journal-title":"Anim. Sci. J."},{"key":"74969_CR5","doi-asserted-by":"publisher","unstructured":"Sun, J. T. et al. Tannin reduces the incidence of polyspermic penetration in porcine oocytes. Antioxidants (Basel) 11, https:\/\/doi.org\/10.3390\/antiox11102027 (2022).","DOI":"10.3390\/antiox11102027"},{"key":"74969_CR6","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1111\/j.1600-079X.2007.00524.x","volume":"44","author":"H Tamura","year":"2008","unstructured":"Tamura, H. et al. Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J. Pineal Res. 44, 280\u2013287. https:\/\/doi.org\/10.1111\/j.1600-079X.2007.00524.x (2008).","journal-title":"J. Pineal Res."},{"key":"74969_CR7","doi-asserted-by":"publisher","unstructured":"Jin, J. X. et al. Melatonin regulates lipid metabolism in porcine oocytes. J. Pineal Res. 62, https:\/\/doi.org\/10.1111\/jpi.12388 (2017).","DOI":"10.1111\/jpi.12388"},{"key":"74969_CR8","doi-asserted-by":"publisher","unstructured":"Jin, J. X. et al. Melatonin regulates lipid metabolism in porcine cumulus-oocyte complexes via the melatonin receptor 2. Antioxid. (Basel) 11, https:\/\/doi.org\/10.3390\/antiox11040687 (2022).","DOI":"10.3390\/antiox11040687"},{"key":"74969_CR9","doi-asserted-by":"publisher","unstructured":"Sun, J. T. et al. Ramelteon reduces oxidative stress by Maintenance of lipid homeostasis in porcine oocytes. Antioxid. (Basel) 11, https:\/\/doi.org\/10.3390\/antiox11091640 (2022).","DOI":"10.3390\/antiox11091640"},{"key":"74969_CR10","doi-asserted-by":"publisher","first-page":"648148","DOI":"10.3389\/fcell.2021.648148","volume":"9","author":"J Wang","year":"2021","unstructured":"Wang, J. et al. Melatonin alleviates the suppressive effect of hypoxanthine on oocyte nuclear maturation and restores meiosis via the melatonin receptor 1 (MT1)-Mediated pathway. Front. Cell. Dev. Biol. 9, 648148. https:\/\/doi.org\/10.3389\/fcell.2021.648148 (2021).","journal-title":"Front. Cell. Dev. Biol."},{"key":"74969_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1477-7827-10-103","volume":"10","author":"RV Sampaio","year":"2012","unstructured":"Sampaio, R. V. et al. MT3 melatonin binding site, MT1 and MT2 melatonin receptors are present in oocyte, but only MT1 is present in bovine blastocyst produced in vitro. Reprod. Biol. Endocrinol. 10, 1\u20137 (2012).","journal-title":"Reprod. Biol. Endocrinol."},{"key":"74969_CR12","doi-asserted-by":"publisher","first-page":"2324","DOI":"10.3390\/ani11082324","volume":"11","author":"S Guo","year":"2021","unstructured":"Guo, S. et al. Melatonin promotes in vitro maturation of vitrified-warmed mouse germinal vesicle oocytes, potentially by reducing oxidative stress through the Nrf2 pathway. Animals 11, 2324 (2021).","journal-title":"Animals"},{"key":"74969_CR13","doi-asserted-by":"publisher","unstructured":"Tian, X. et al. Beneficial effects of melatonin on the in vitro maturation of sheep oocytes and its relation to melatonin receptors. Int. J. Mol. Sci. 18, https:\/\/doi.org\/10.3390\/ijms18040834 (2017).","DOI":"10.3390\/ijms18040834"},{"key":"74969_CR14","doi-asserted-by":"publisher","unstructured":"Lee, S. et al. Melatonin influences the sonic hedgehog signaling pathway in porcine cumulus oocyte complexes. J. Pineal Res. 63, https:\/\/doi.org\/10.1111\/jpi.12424 (2017).","DOI":"10.1111\/jpi.12424"},{"key":"74969_CR15","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1111\/j.1600-079X.2008.00602.x","volume":"46","author":"JT Kang","year":"2009","unstructured":"Kang, J. T. et al. Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells. J. Pineal Res. 46, 22\u201328. https:\/\/doi.org\/10.1111\/j.1600-079X.2008.00602.x (2009).","journal-title":"J. Pineal Res."},{"key":"74969_CR16","doi-asserted-by":"publisher","first-page":"648209","DOI":"10.3389\/fcell.2021.648209","volume":"9","author":"T Zhu","year":"2021","unstructured":"Zhu, T. et al. Melatonin modulates lipid metabolism in porcine cumulus-oocyte complex via its receptors. Front. Cell. Dev. Biol. 9, 648209. https:\/\/doi.org\/10.3389\/fcell.2021.648209 (2021).","journal-title":"Front. Cell. Dev. Biol."},{"key":"74969_CR17","doi-asserted-by":"publisher","first-page":"E1","DOI":"10.2527\/jas.2006-432","volume":"85","author":"AJ Watson","year":"2007","unstructured":"Watson, A. J. Oocyte cytoplasmic maturation: a key mediator of oocyte and embryo developmental competence. J. Anim. Sci. 85, E1-3. https:\/\/doi.org\/10.2527\/jas.2006-432 (2007).","journal-title":"J. Anim. Sci."},{"key":"74969_CR18","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1002\/mrd.21102","volume":"77","author":"M Paczkowski","year":"2010","unstructured":"Paczkowski, M. & Krisher, R. Aberrant protein expression is associated with decreased developmental potential in porcine cumulus\u2013oocyte complexes. Mol. Reprod. Dev. 77, 51\u201358 (2010).","journal-title":"Mol. Reprod. Dev."},{"key":"74969_CR19","doi-asserted-by":"publisher","unstructured":"Zhao, X. M. et al. Melatonin improves the fertilization capacity and developmental ability of bovine oocytes by regulating cytoplasmic maturation events. J. Pineal Res. 64, https:\/\/doi.org\/10.1111\/jpi.12445 (2018).","DOI":"10.1111\/jpi.12445"},{"key":"74969_CR20","doi-asserted-by":"publisher","first-page":"e0256701","DOI":"10.1371\/journal.pone.0256701","volume":"16","author":"JC Gutierrez-Anez","year":"2021","unstructured":"Gutierrez-Anez, J. C. et al. Melatonin improves rate of monospermic fertilization and early embryo development in a bovine IVF system. PLoS ONE 16, e0256701. https:\/\/doi.org\/10.1371\/journal.pone.0256701 (2021).","journal-title":"PLoS One"},{"key":"74969_CR21","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1016\/j.rbmo.2013.10.016","volume":"28","author":"L Mao","year":"2014","unstructured":"Mao, L., Lou, H., Lou, Y., Wang, N. & Jin, F. Behaviour of cytoplasmic organelles and cytoskeleton during oocyte maturation. Reprod. Biomed. Online 28, 284\u2013299. https:\/\/doi.org\/10.1016\/j.rbmo.2013.10.016 (2014).","journal-title":"Reprod. Biomed. Online"},{"key":"74969_CR22","doi-asserted-by":"publisher","unstructured":"Guzel, E. et al. Endoplasmic reticulum stress and homeostasis in reproductive physiology and pathology. Int. J. Mol. Sci. 18, https:\/\/doi.org\/10.3390\/ijms18040792 (2017).","DOI":"10.3390\/ijms18040792"},{"key":"74969_CR23","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.yexcr.2016.07.005","volume":"347","author":"OD Bello","year":"2016","unstructured":"Bello, O. D. et al. Rab3A, a possible marker of cortical granules, participates in cortical granule exocytosis in mouse eggs. Exp. Cell. Res. 347, 42\u201351. https:\/\/doi.org\/10.1016\/j.yexcr.2016.07.005 (2016).","journal-title":"Exp. Cell. Res."},{"key":"74969_CR24","doi-asserted-by":"crossref","unstructured":"Roth, Z. Symposium review: reduction in oocyte developmental competence by stress is associated with alterations in mitochondrial function. J. Dairy Sci. 101, 3642\u20133654 (2018).","DOI":"10.3168\/jds.2017-13389"},{"key":"74969_CR25","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1095\/biolreprod.110.085647","volume":"84","author":"PS Tsai","year":"2011","unstructured":"Tsai, P. S., van Haeften, T. & Gadella, B. M. Preparation of the cortical reaction: maturation-dependent migration of SNARE proteins, clathrin, and complexin to the porcine oocyte\u2019s surface blocks membrane traffic until fertilization. Biol. Reprod. 84, 327\u2013335. https:\/\/doi.org\/10.1095\/biolreprod.110.085647 (2011).","journal-title":"Biol. Reprod."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-74969-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-74969-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-74969-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T06:08:44Z","timestamp":1728367724000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-74969-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,5]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["74969"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-74969-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,5]]},"assertion":[{"value":"23 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 September 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 October 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"23153"}}