{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T17:01:51Z","timestamp":1793638911186,"version":"4.5.1"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Henan Province Medical Science and Technology Research Plan Joint Construction Project","award":["LHGJ20220157"],"award-info":[{"award-number":["LHGJ20220157"]}]},{"name":"Henan Province Medical Science and Technology Research Plan Joint Construction Project","award":["LHGJ20210134"],"award-info":[{"award-number":["LHGJ20210134"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sulfadoxine-pyrimethamine (SP) is a key drug recommended by the World Health Organization for the chemoprevention of malaria. However, the strategy is affected by the parasite resistance to SP. This study evaluated\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    dihydrofolate reductase (\n                    <jats:italic>Pfdhfr<\/jats:italic>\n                    ) and dihydropteroate synthase (\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    ) genes, associated with SP resistance, from 508\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    isolates imported from West African countries to Henan Province, China, during 2012\u20132022. High mutant prevalence of the genes\n                    <jats:italic>Pfdhfr<\/jats:italic>\n                    (94.7%) and\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    (96.8%) was observed. The mutants\n                    <jats:italic>Pfdhfr<\/jats:italic>\n                    N51I, C59R, S108N, and\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    A437G were at high frequency in all countries analyzed. The overall prevalence of the mutant\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    K540E was low (3.4%), but with a high frequency in Liberia (24.3%). The frequency of mutants\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    I431V, A581G, and A613S was 11.7%, 9.8%, and 16.2%, respectively, all of which had the highest mutant prevalence in Nigeria. The mutant\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    A581G and A613S were identified in the absence of K540E. The partially resistant haplotype (I\n                    <jats:sub>51<\/jats:sub>\n                    R\n                    <jats:sub>59<\/jats:sub>\n                    N\n                    <jats:sub>108<\/jats:sub>\n                    - G\n                    <jats:sub>437<\/jats:sub>\n                    ) was the most common (72.6%), and the fully resistant haplotype (I\n                    <jats:sub>51<\/jats:sub>\n                    R\n                    <jats:sub>59<\/jats:sub>\n                    N\n                    <jats:sub>108<\/jats:sub>\n                    - G\n                    <jats:sub>437<\/jats:sub>\n                    E\n                    <jats:sub>540<\/jats:sub>\n                    ) had a low prevalence of 3.4% and mainly occurred in Liberia. No super resistant haplotype was identified. The mutant\n                    <jats:italic>Pfdhps<\/jats:italic>\n                    I431V and the octuple mutant haplotype I\n                    <jats:sub>51<\/jats:sub>\n                    R\n                    <jats:sub>59<\/jats:sub>\n                    N\n                    <jats:sub>108<\/jats:sub>\n                    - V\n                    <jats:sub>431<\/jats:sub>\n                    A\n                    <jats:sub>436<\/jats:sub>\n                    G\n                    <jats:sub>437<\/jats:sub>\n                    G\n                    <jats:sub>581<\/jats:sub>\n                    S\n                    <jats:sub>613<\/jats:sub>\n                    deserve more attention. In areas of high SP resistance, the intervention still reduces low birthweight and maternal anaemia. SP should continue to be used in areas of high SP resistance until more effective alternatives for malaria chemoprevention are found. It is important to continuously monitor the molecular markers associated with SP resistance to better implement intermittent preventive treatment policies in pregnancy (IPTp) and infants (IPTi).\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-75828-w","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:16:02Z","timestamp":1730679362000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Prevalence of molecular markers of sulfadoxine-pyrimethamine resistance in Plasmodium falciparum isolates from West Africa during 2012\u20132022"],"prefix":"10.1038","volume":"14","author":[{"given":"Ruimin","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penghui","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shucheng","family":"Ruan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiquan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deling","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,4]]},"reference":[{"key":"75828_CR1","unstructured":"World Health Organization. World Malaria Report 2022. https:\/\/www.who.int\/publications\/i\/item\/9789240064898 (2023)."},{"key":"75828_CR2","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/0035-9203(90)90246-b","volume":"84","author":"A Bjorkman","year":"1990","unstructured":"Bjorkman, A. & Phillips-Howard, P. A. The epidemiology of drug-resistant malaria. Trans. R Soc. Trop. Med. Hyg. 84, 177\u2013180. https:\/\/doi.org\/10.1016\/0035-9203(90)90246-b (1990).","journal-title":"Trans. R Soc. Trop. Med. Hyg."},{"key":"75828_CR3","doi-asserted-by":"publisher","first-page":"857","DOI":"10.4269\/ajtmh.13-0129","volume":"89","author":"JA Flegg","year":"2013","unstructured":"Flegg, J. A. et al. Trends in antimalarial drug use in Africa. Am. J. Trop. Med. Hyg. 89, 857\u2013865. https:\/\/doi.org\/10.4269\/ajtmh.13-0129 (2013).","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"75828_CR4","unstructured":"World Health Organization. WHO policy brief for the implementation of intermittent preventive treatment of malaria in pregnancy using sulfadoxine-pyrimethamine (IPTp-SP). https:\/\/www.who.int\/publications\/i\/item\/WHO-HTM-GMP-2014.4 (2013)."},{"key":"75828_CR5","doi-asserted-by":"publisher","first-page":"e12618","DOI":"10.1371\/journal.pone.0012618","volume":"5","author":"JT Griffin","year":"2010","unstructured":"Griffin, J. T. et al. Protective efficacy of intermittent preventive treatment of malaria in infants (IPTi) using sulfadoxine-pyrimethamine and parasite resistance. PloS One 5, e12618. https:\/\/doi.org\/10.1371\/journal.pone.0012618 (2010).","journal-title":"PloS One"},{"key":"75828_CR6","unstructured":"World Health Organization. Intermittent preventive treatment for infants using sulfadoxine-pyrimethamine (IPTi-SP) for malaria control in Africa. https:\/\/www.who.int\/publications\/i\/item\/WHO-IVB-11.07 (2011)."},{"key":"75828_CR7","unstructured":"World Health Organization. Guidelines for malaria. https:\/\/www.who.int\/publications\/i\/item\/guidelines-for-malaria (2023)."},{"key":"75828_CR8","doi-asserted-by":"publisher","first-page":"13944","DOI":"10.1073\/pnas.94.25.13944","volume":"94","author":"T Triglia","year":"1997","unstructured":"Triglia, T., Menting, J. G. & Wilson, C. Mutations in dihydropteroate synthase are responsible for sulfone and sulfonamide resistance in Plasmodium falciparum. Proc. Natl. Acad. Sci. U.S.A. 94, 13944\u201313949. https:\/\/doi.org\/10.1073\/pnas.94.25.13944 (1997).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"75828_CR9","doi-asserted-by":"publisher","first-page":"9114","DOI":"10.1073\/pnas.85.23.9114","volume":"85","author":"DS Peterson","year":"1988","unstructured":"Peterson, D. S., Walliker, D. & Wellems, T. E. Evidence that a point mutation in dihydrofolate reductase-thymidylate synthase confers resistance to pyrimethamine in falciparum malaria. Proc. Natl. Acad. Sci. U.S.A.. 85, 9114\u20139118. https:\/\/doi.org\/10.1073\/pnas.85.23.9114 (1988).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"75828_CR10","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1046\/j.1365-2958.1997.2821646.x","volume":"23","author":"P Wang","year":"1997","unstructured":"Wang, P., Read, M., Sims, P. F. G. & Hyde, J. E. Sulfadoxine resistance in the human malaria parasite Plasmodium falciparum is determined by mutations in dihydropteroate synthetase and an additional factor associated with folate utilization. Mol. Microbiol. 23, 979\u2013986. https:\/\/doi.org\/10.1046\/j.1365-2958.1997.2821646.x (1997).","journal-title":"Mol. Microbiol."},{"key":"75828_CR11","doi-asserted-by":"publisher","first-page":"7389","DOI":"10.1038\/s41598-017-06708-9","volume":"7","author":"LC Okell","year":"2017","unstructured":"Okell, L. C., Griffin, J. T. & Roper, C. Mapping sulphadoxine-pyrimethamine resistant Plasmodium falciparum malaria in infected humans and in parasite populations in Africa. Sci. Rep. 7, 7389. https:\/\/doi.org\/10.1038\/s41598-017-06708-9 (2017).","journal-title":"Sci. Rep."},{"key":"75828_CR12","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1007\/s00436-018-5754-5","volume":"117","author":"E Esu","year":"2018","unstructured":"Esu, E. et al. Prevalence of the Pfdhfr and Pfdhps mutations among asymptomatic pregnant women in southeast Nigeria. Parasitol. Res. 117, 801\u2013807. https:\/\/doi.org\/10.1007\/s00436-018-5754-5 (2018).","journal-title":"Parasitol. Res."},{"key":"75828_CR13","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1093\/cid\/civ881","volume":"62","author":"M Desai","year":"2016","unstructured":"Desai, M. et al. Impact of sulfadoxine-pyrimethamine resistance on effectiveness of intermittent preventive therapy for malaria in pregnancy at clearing infections and preventing low birth weight. Clin. Infect. Dis. 62, 323\u2013333. https:\/\/doi.org\/10.1093\/cid\/civ881 (2016).","journal-title":"Clin. Infect. Dis."},{"key":"75828_CR14","doi-asserted-by":"publisher","first-page":"3405","DOI":"10.1128\/AAC.00024-09","volume":"53","author":"CJ Sutherland","year":"2009","unstructured":"Sutherland, C. J. et al. Novel pfdhps haplotypes among imported cases of Plasmodium falciparum malaria in the United Kingdom. Agents Chemother. 53, 3405\u20133410. https:\/\/doi.org\/10.1128\/AAC.00024-09 (2009).","journal-title":"Agents Chemother."},{"key":"75828_CR15","doi-asserted-by":"publisher","first-page":"2566","DOI":"10.1093\/jac\/dkv160","volume":"70","author":"P Chauvin","year":"2016","unstructured":"Chauvin, P. et al. Prevalence of Plasmodium falciparum parasites resistant to sulfadoxine\/pyrimethamine in pregnant women in Yaound\u00e9, Cameroon: emergence of highly resistant pfdhfr\/pfdhps alleles. J. Antimicrob. Chemother. 70, 2566\u20132571. https:\/\/doi.org\/10.1093\/jac\/dkv160 (2016).","journal-title":"J. Antimicrob. Chemother."},{"key":"75828_CR16","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.ijpddr.2016.08.004","volume":"6","author":"MC Oguike","year":"2016","unstructured":"Oguike, M. C. et al. Molecular determinants of sulfadoxine-pyrimethamine resistance in Plasmodium falciparum in Nigeria and the regional emergence of dhps 431V. J. Parasitol. Drugs Drug Resist. 6, 220\u2013229. https:\/\/doi.org\/10.1016\/j.ijpddr.2016.08.004 (2016).","journal-title":"J. Parasitol. Drugs Drug Resist."},{"key":"75828_CR17","doi-asserted-by":"publisher","first-page":"e0200522","DOI":"10.1128\/spectrum.02005-22","volume":"10","author":"X Wang","year":"2022","unstructured":"Wang, X. et al. Molecular determinants of sulfadoxine pyrimethamine resistance in Plasmodium falciparum isolates from Central Africa between 2016 and 2021: Wide geographic spread of highly mutated Pfdhfr and Pfdhps alleles. Microbiol. Spectr. 10, e0200522. https:\/\/doi.org\/10.1128\/spectrum.02005-22 (2022).","journal-title":"Microbiol. Spectr."},{"key":"75828_CR18","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1093\/jac\/dkac432","volume":"78","author":"O Cohen","year":"2023","unstructured":"Cohen, O. et al. Effect of sulfadoxine-pyrimethamine chemoprophylaxis in pregnant women on selection of the new P. falciparum dhps quintuple mutant carrying the I431V mutation. Antimicrob. Chemother. 78, 665\u2013668. https:\/\/doi.org\/10.1093\/jac\/dkac432 (2023).","journal-title":"Antimicrob. Chemother."},{"key":"75828_CR19","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1080\/00034980120103234","volume":"95","author":"SA Omar","year":"2001","unstructured":"Omar, S. A., Adagu, I. S., Gump, D. W., Ndaru, N. P. & Warhurst, D. C. Plasm falciparumiparum in Kenya: High prevalence of drug-resistance-associated polymorphisms in hospital admissions with severe malaria in an epidemic area. Ann. Trop. Med. Parasitol. 95, 661\u2013669. https:\/\/doi.org\/10.1080\/00034980120103234 (2001).","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"75828_CR20","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1111\/j.1365-3156.2004.01239.x","volume":"9","author":"SG Staedke","year":"2004","unstructured":"Staedke, S. G. et al. Relationship between age, molecular markers, and response to sulphadoxine-pyrimethamine treatment in Kampala, Uganda. Trop. Med. Int. Health. 9, 624\u2013629. https:\/\/doi.org\/10.1111\/j.1365-3156.2004.01239.x (2004).","journal-title":"Trop. Med. Int. Health"},{"key":"75828_CR21","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1086\/338566","volume":"185","author":"SG Staedke","year":"2002","unstructured":"Staedke, S. G. et al. Molecular markers for failure of sulfadoxine-pyrimethamine and chlorproguanil-dapsone treatment of Plasmodium falciparum malaria. J. Infect. Dis. 185, 380\u2013388. https:\/\/doi.org\/10.1086\/338566 (2002).","journal-title":"J. Infect. Dis."},{"key":"75828_CR22","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1016\/S1473-3099(12)70222-0","volume":"12","author":"TP Eisele","year":"2012","unstructured":"Eisele, T. P. et al. Malaria prevention in pregnancy, birthweight, and neonatal mortality: A meta-analysis of 32 national cross-sectional datasets in Africa. Lancet Infect. Dis. 12, 942\u2013949. https:\/\/doi.org\/10.1016\/S1473-3099(12)70222-0 (2012).","journal-title":"Lancet Infect. Dis."},{"key":"75828_CR23","doi-asserted-by":"publisher","first-page":"4517","DOI":"10.2147\/IDR.S375197","volume":"15","author":"S Nikiema","year":"2022","unstructured":"Nikiema, S. et al. Seasonal malaria chemoprevention implementation: Effect on Malaria incidence and immunity in a context of expansion of P. falciparum resistant genotypes with potential reduction of the effectiveness in Sub-saharan Africa. Infect. Drug Resist. 15, 4517\u20134527. https:\/\/doi.org\/10.2147\/IDR.S375197 (2022).","journal-title":"Infect. Drug Resist."},{"key":"75828_CR24","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1016\/s1473-3099(18)30732-1","volume":"19","author":"AM van Eijk","year":"2019","unstructured":"van Eijk, A. M. et al. Effect of Plasmodium falciparum sulfadoxine-pyrimethamine resistance on the effectiveness of intermittent preventive therapy for malaria in pregnancy in Africa: A systematic review and meta-analysis. Lancet Infect. Dis. 19, 546\u2013556. https:\/\/doi.org\/10.1016\/s1473-3099(18)30732-1 (2019).","journal-title":"Lancet Infect. Dis."},{"key":"75828_CR25","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1073\/pnas.94.4.1124","volume":"94","author":"W Sirawaraporn","year":"1997","unstructured":"Sirawaraporn, W., Sathitkul, T., Sirawaraporn, R., Yuthavong, Y. & Santi, D. V. Antifolate-resistant mutants of Plasmodium falciparum dihydrofolate reductase. Proc. Natl. Acad. Sci. U.S.A.. 94, 1124\u20131129. https:\/\/doi.org\/10.1073\/pnas.94.4.1124 (1997).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"75828_CR26","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1186\/s12936-023-04487-5","volume":"22","author":"AJ Adegbola","year":"2023","unstructured":"Adegbola, A. J. et al. A snapshot of the prevalence of dihydropteroate synthase-431V mutation and other sulfadoxine-pyrimethamine resistance markers in Plasmodium falciparum isolates in Nigeria. Malar. J. 22, 71. https:\/\/doi.org\/10.1186\/s12936-023-04487-5 (2023).","journal-title":"Malar. J."},{"key":"75828_CR27","doi-asserted-by":"publisher","first-page":"e0278928","DOI":"10.1371\/journal.pone.0278928","volume":"17","author":"S Srisutham","year":"2022","unstructured":"Srisutham, S. et al. Ten-year persistence and evolution of Plasmodium falciparum antifolate and antisulfonamide resistance markers pfdhfr and pfdhps in three Asian countries. PLoS One 17, e0278928. https:\/\/doi.org\/10.1371\/journal.pone.0278928 (2022).","journal-title":"PLoS One"},{"key":"75828_CR28","doi-asserted-by":"publisher","first-page":"109","DOI":"10.3347\/kjp.2022.60.2.109","volume":"60","author":"J Kuesap","year":"2022","unstructured":"Kuesap, J., Suphakhonchuwong, N., Kalawong, L. & Khumchum, N. Molecular markers for sulfadoxine\/pyrimethamine and chloroquine resistance in Plasmodium falciparum in Thailand. Korean J. Parasitol. 60, 109\u2013116. https:\/\/doi.org\/10.3347\/kjp.2022.60.2.109 (2022).","journal-title":"Korean J. Parasitol."},{"key":"75828_CR29","doi-asserted-by":"publisher","first-page":"e0271489","DOI":"10.1371\/journal.pone.0271489","volume":"17","author":"DK Dosoo","year":"2022","unstructured":"Dosoo, D. K. et al. The prevalence of molecular markers of resistance to sulfadoxine-pyrimethamine among pregnant women at first antenatal clinic attendance and delivery in the forest-savannah area of Ghana. PLoS One 17, e0271489. https:\/\/doi.org\/10.1371\/journal.pone.0271489 (2022).","journal-title":"PLoS One"},{"key":"75828_CR30","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1016\/j.pt.2013.08.002","volume":"29","author":"I Naidoo","year":"2013","unstructured":"Naidoo, I. & Roper, C. Mapping \u2018partially resistant\u2019, \u2018fully resistant\u2019, and \u2018super resistant\u2019 malaria. Trends Parasitol. 29, 505\u2013515. https:\/\/doi.org\/10.1016\/j.pt.2013.08.002 (2013).","journal-title":"Trends Parasitol."},{"key":"75828_CR31","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1186\/1475-2875-8-89","volume":"8","author":"S Picot","year":"2009","unstructured":"Picot, S. et al. A systematic review and meta-analysis of evidence for correlation between molecular markers of parasite resistance and treatment outcome in falciparum malaria. Malar. J. 8, 89. https:\/\/doi.org\/10.1186\/1475-2875-8-89 (2009).","journal-title":"Malar. J."},{"key":"75828_CR32","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.3201\/eid1909.130133","volume":"19","author":"DTR Minja","year":"2013","unstructured":"Minja, D. T. R. et al. Plasm falciparumiparum mutant haplotype infection during pregnancy associated with reduced birthweight, Tanzania. Emerg. Infect. Dis. 19, 1446\u20131454. https:\/\/doi.org\/10.3201\/eid1909.130133 (2013).","journal-title":"Emerg. Infect. Dis."},{"key":"75828_CR33","doi-asserted-by":"publisher","first-page":"1997","DOI":"10.1093\/infdis\/jiu836","volume":"211","author":"J Gutman","year":"2015","unstructured":"Gutman, J. et al. The A581G mutation in the gene encoding Plasmodium falciparum dihydropteroate synthetase reduces the effectiveness of sulfadoxine-pyrimethamine preventive therapy in Malawian pregnant women. J. J. Infect. Dis. 211, 1997\u20132005. https:\/\/doi.org\/10.1093\/infdis\/jiu836 (2015).","journal-title":"J. J. Infect. Dis."},{"key":"75828_CR34","doi-asserted-by":"publisher","first-page":"9027","DOI":"10.1073\/pnas.0901415106","volume":"106","author":"WE Harrington","year":"2009","unstructured":"Harrington, W. E. et al. Competitive facilitation of drug-resistant Plasmodium falciparum malaria parasites in pregnant women who receive preventive treatment. Proc. Natl. Acad. Sci. U.S.A. 106, 9027\u20139032. https:\/\/doi.org\/10.1073\/pnas.0901415106 (2009).","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"75828_CR35","unstructured":"World Health Organization. Global report on antimalarial efficacy and drug resistance: 2000\u20132010. https:\/\/www.who.int\/publications\/i\/item\/9789241500470 (2010)."},{"key":"75828_CR36","unstructured":"World Health Organization. Meeting report of the Evidence Review Group on intermittent preventive treatment (IPT) of malaria in pregnancy. https:\/\/www.who.int\/publications\/m\/item\/meeting-report-of-the-evidence-review-group-on-intermittent-preventive-treatment-of-malaria-in-pregnancy (2013)."},{"key":"75828_CR37","doi-asserted-by":"publisher","first-page":"e1010317","DOI":"10.1371\/journal.pcbi.1010317","volume":"18","author":"JA Flegg","year":"2022","unstructured":"Flegg, J. A. et al. Spatiotemporal spread of Plasmodium falciparum mutations for resistance to sulfadoxine-pyrimethamine across Africa, 1990\u20132020. PLoS Comput. Biol. 18, e1010317. https:\/\/doi.org\/10.1371\/journal.pcbi.1010317 (2022).","journal-title":"PLoS Comput. Biol."},{"key":"75828_CR38","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.ijpddr.2016.08.004","volume":"6","author":"MC Oguike","year":"2016","unstructured":"Oguike, M. C. et al. Molecular determinants of sulfadoxine-pyrimethamine resistance in Plasmodium falciparum in Nigeria and the regional emergence of dhps 431V. Int. J. Parasitol. Drugs Drug Resist. 6, 220\u2013229. https:\/\/doi.org\/10.1016\/j.ijpddr.2016.08.004 (2016).","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"75828_CR39","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/S1473-3099(22)00593-X","volume":"23","author":"KB Beshir","year":"2023","unstructured":"Beshir, K. B. et al. Prevalence of Plasmodium falciparum haplotypes associated with resistance to sulfadoxine\u2013pyrimethamine and amodiaquine before and after upscaling of seasonal malaria chemoprevention in seven African countries: A genomic surveillance study. Lancet Infect. Dis. 23, 361\u2013370. https:\/\/doi.org\/10.1016\/S1473-3099(22)00593-X (2023).","journal-title":"Lancet Infect. Dis."},{"key":"75828_CR40","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.actatropica.2007.04.008","volume":"103","author":"R Tahar","year":"2007","unstructured":"Tahar, R. & Basco, L. K. Molecular epidemiology of malaria in Cameroon XXVII. Clinical and parasitological response to sulfadoxine-pyrimethamine treatment and Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase alleles in Cameroonian children. Acta Trop. 103, 81\u201389. https:\/\/doi.org\/10.1016\/j.actatropica.2007.04.008 (2007).","journal-title":"Acta Trop."},{"key":"75828_CR41","doi-asserted-by":"publisher","first-page":"644576","DOI":"10.3389\/fcimb.2021.644576","volume":"11","author":"D Zhao","year":"2021","unstructured":"Zhao, D. et al. Surveillance of antimalarial drug-resistance genes in imported Plasmodium falciparum isolates from Nigeria in Henan, China, 2012\u20132019. Front. Cell. Infect. Microbiol. 11, 644576. https:\/\/doi.org\/10.3389\/fcimb.2021.644576 (2021).","journal-title":"Front. Cell. Infect. Microbiol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-75828-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-75828-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-75828-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:18:08Z","timestamp":1730679488000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-75828-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,4]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["75828"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-75828-w","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4453141\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,4]]},"assertion":[{"value":"21 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 October 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This study was approved by the Ethical Committee of Henan Province Center for Disease Control and Prevention. The patients\/participants provided their written informed consent to participate in this study. All the identifiers of patients were removed from the data. All the methods were performed in accordance with the relevant guidelines and regulations of the Henan Province Center for Disease Control and Prevention.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics statement"}}],"article-number":"26567"}}