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We aimed to establish the observer repeatability of coronary, carotid and cerebral <jats:sup>18<\/jats:sup>F-GP1 uptake in patients presenting with acute myocardial infarction or ischaemic stroke. Forty-three patients presenting with acute myocardial infarction or ischaemic stroke underwent hybrid positron emission tomography (PET) and computed tomography (CT) angiography. Qualitative and quantitative assessment of <jats:sup>18<\/jats:sup>F-GP1 uptake was performed on coronary arteries, carotid arteries and brain parenchyma. Qualitative uptake of <jats:sup>18<\/jats:sup>F-GP1 had excellent intraobserver and interobserver agreement, with complete agreement for the presence or absence of visual <jats:sup>18<\/jats:sup>F-GP1 uptake. For quantitative analysis, there were excellent intraclass correlation coefficients for intraobserver repeatability for coronary artery, carotid artery and brain parenchymal SUV<jats:sub>max<\/jats:sub> and TBR<jats:sub>max<\/jats:sub> measurements (all \u2265\u20090.92). Coronary artery and brain parenchymal analyses showed the strongest agreement in SUV<jats:sub>max<\/jats:sub> values with mean biases of \u2212\u20090.04 (limits of agreement \u2212\u20090.21 to 0.20) and 0.02 (limits of agreement \u2212\u20090.29 to 0.32) respectively. There was good interclass correlation coefficients for interobserver repeatability for coronary artery, carotid artery and brain parenchymal SUV<jats:sub>max<\/jats:sub> and TBR<jats:sub>max<\/jats:sub> measurements (all \u2265\u20090.89). The strongest interobserver agreement was seen with brain parenchymal SUV<jats:sub>max<\/jats:sub> (mean SUV<jats:sub>max<\/jats:sub> 1.95\u2009\u00b1\u20090.94) and TBR<jats:sub>max<\/jats:sub> (mean TBR<jats:sub>max<\/jats:sub> 9.55\u2009\u00b1\u20096.56) with mean biases of \u2212\u20090.05 (limits of agreement \u2212\u20090.37 to 0.27) and 0.04 (limits of agreement \u2212\u20090.59 to 0.52) respectively. Visual qualitative and quantitative <jats:sup>18<\/jats:sup>F-GP1 PET-CT image analyses provide robust and repeatable measurements of activated platelets and thrombi within the coronary arteries, carotid arteries and brain parenchyma.<\/jats:p>","DOI":"10.1038\/s41598-024-77151-w","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T07:05:11Z","timestamp":1730790311000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Qualitative and quantitative analysis of 18F-GP1 positron emission tomography in thrombotic cardiovascular disease"],"prefix":"10.1038","volume":"14","author":[{"given":"Beth","family":"Whittington","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelos","family":"Tzolos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shruti","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Bing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Andrews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krithika","family":"Loganath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Craig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Balmforth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Lucatelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark G","family":"MacAskill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana A. 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NK and AS are employees of Life Molecular Imaging who provided reagents for 18\u00a0F-GP1 production.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"26792"}}