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This study aimed to investigate the potential role of oysters sold in Egypt as a source for <jats:italic>E. coli<\/jats:italic>, identify their resistance and virulence-associated gene profiles, and assess associated zoonotic risks. A total of 33 pooled fresh oyster samples were obtained from various retail fish markets in Egypt and examined bacteriologically for the presence of <jats:italic>E. coli<\/jats:italic>. Antimicrobial resistance was performed by the disk-diffusion method, and the multiple antibiotic resistance index (MAR) was calculated. All isolates were screened for extended-spectrum beta-lactamase (ESBL) (<jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2212M<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>OXA\u22121<\/jats:sub>), plasmid-mediated AmpC <jats:italic>bla<\/jats:italic><jats:sub>CMY\u22122<\/jats:sub>, and carbapenemases (<jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>VIM<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>OXA\u221248<\/jats:sub>) genes by Polymerase chain reaction. Moreover, the presence of virulence-encoding genes was investigated. The virulent MDR strains were clustered using R with the pheatmap package. The prevalence of <jats:italic>E. coli<\/jats:italic> was 72.7% (24 out of 33), with 66.7% of the isolates classified as multi-drug resistant, and 75% exhibited MAR values exceeding the 0.2 threshold. Different antimicrobial sensitivity phenotypes and genotype profiles were identified in <jats:italic>E. coli<\/jats:italic> isolates. The most prevalent gene detected among all isolates was <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> (22\/24, 91.7%). Notably, all non-ESBL producers were positive for <jats:italic>bla<\/jats:italic><jats:sub>CMY2<\/jats:sub>. Carbapenem-resistant and carbapenem-intermediate strains were carbapenemase producers, with the predominance of the <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub> gene (11\/24, 45.8%). Remarkably, twelve out of sixteen virulence genes were identified, with <jats:italic>papC<\/jats:italic> (21\/24, 87.5%) and <jats:italic>sfa<\/jats:italic> (16\/24, 66.7%) genes being the most prevalent. Most isolates carry virulence genes primarily associated with extra-intestinal pathogenic <jats:italic>E. coli<\/jats:italic> (ExPEC) (87.5%) and enteropathogenic (EPEC) (70.8%) pathotypes. Four <jats:italic>E. coli<\/jats:italic> isolates exhibit cluster patterns. This study provides the first insight into the emergence of virulent MDR <jats:italic>E. coli<\/jats:italic> among oysters in Egypt. It underscores the potential role of oysters as a source for disseminating these strains within aquatic ecosystems, presenting a possible threat to public health.<\/jats:p>","DOI":"10.1038\/s41598-024-77519-y","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T14:03:26Z","timestamp":1730901806000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Public health concern of antimicrobial resistance and virulence determinants in E. coli isolates from oysters in Egypt"],"prefix":"10.1038","volume":"14","author":[{"given":"Rahma","family":"Mohammed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara M.","family":"Nader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia A.","family":"Hamza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha A.","family":"Sabry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,6]]},"reference":[{"key":"77519_CR1","doi-asserted-by":"publisher","first-page":"415","DOI":"10.3390\/microorganisms11020415","volume":"11","author":"S Freire","year":"2023","unstructured":"Freire, S. et al. 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