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Therefore, inhibition of glucosyltransferase activity impairs the virulence of cariogenic biofilms, which can be used to prevent dental caries. We evaluated the anti-bacterial, anti-biofilm, and anti-glucosyltransferases effects of nanoliposomal herbal aqueous extracts of Liquorice (Glycyrrhiza glabra; G. glabra), Ginger (Zingiber officinale; Z. officinale), Pomegranate (Punica granatum; P. granatum), and Rose (Rosa damascene; R. damascene) via minimum bactericidal concentration and minimum inhibitory concentration against <jats:italic>Streptococcus mutans<\/jats:italic> strain ATCC 35,668. An anti-biofilm assay was performed using a minimum biofilm inhibitory concentration test. Among herbs, only P. granatum showed an antibacterial effect. Therefore, a nanoliposomal formulation of <jats:italic>P. granatum<\/jats:italic> was developed and characterized. Its effect on <jats:italic>S.mutans<\/jats:italic> glucosyltransferases was assessed by measuring glucan amount. The nanoliposomal formulation of P.granatum showed a significantly higher anti-biofilm effect than P. granatum aqueous extract. Their similar potential in blocking glucosyltransferases showed that the nanoliposomal formulation of P.granatum blocked other pathways rather than blocking glucosyltransferases for its anti-biofilm effect. Collectively, the nanoliposomal formulation of <jats:italic>P.granatum<\/jats:italic>, due to its anti- <jats:italic>Streptococcus mutans<\/jats:italic> characteristics, would be a production which open a new horizon for the oral pharmaceutical industry.<\/jats:p>","DOI":"10.1038\/s41598-024-78728-1","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T20:10:50Z","timestamp":1731096650000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Anti-biofilm and anti-glucosyltransferase effects of nano liposomal plant extracts against Streptococcus mutans"],"prefix":"10.1038","volume":"14","author":[{"given":"Faraz","family":"Radmand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milad","family":"Baseri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Yousef","family":"Memar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Ebrahimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamed","family":"Hamishehkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solmaz","family":"Asnaashari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amirreza","family":"Naseri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Kouhsoltani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"issue":"8","key":"78728_CR1","doi-asserted-by":"publisher","first-page":"941","DOI":"10.3109\/13880209.2011.649430","volume":"50","author":"N Joycharat","year":"2012","unstructured":"Joycharat, N. et al. 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