{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T13:42:44Z","timestamp":1793194964622,"version":"4.5.0"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T00:00:00Z","timestamp":1731715200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T00:00:00Z","timestamp":1731715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PRE2019-089985"],"award-info":[{"award-number":["PRE2019-089985"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"name":"\"Ministerio de Ciencia, Innovacio\u0301n y Universidades (MCIU), Agencia Estatal de Investigacio\u0301n (AEI)\u201d and \u201cFondo Europeo de Desarrollo Regional (FEDER)\u201d","award":["RTI2018\u2013094071-B-C21"],"award-info":[{"award-number":["RTI2018\u2013094071-B-C21"]}]},{"name":"\"Ministerio de Ciencia, Innovacio\u0301n y Universidades (MCIU), Agencia Estatal de Investigacio\u0301n (AEI)\u201d and \u201cFondo Europeo de Desarrollo Regional (FEDER)\u201d","award":["RTI2018\u2013094071-B-C21"],"award-info":[{"award-number":["RTI2018\u2013094071-B-C21"]}]},{"name":"\"Ministerio de Ciencia, Innovacio\u0301n y Universidades (MCIU), Agencia Estatal de Investigacio\u0301n (AEI)\u201d and \u201cFondo Europeo de Desarrollo Regional (FEDER)\u201d","award":["RTI2018\u2013094071-B-C21"],"award-info":[{"award-number":["RTI2018\u2013094071-B-C21"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-79481-1","type":"journal-article","created":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T05:50:54Z","timestamp":1731736254000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Enrichment protocols for human conjunctival extracellular vesicles and their characterization"],"prefix":"10.1038","volume":"14","author":[{"given":"Ismael","family":"Romero-Castillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"L\u00f3pez-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Diebold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Garc\u00eda-Posadas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,16]]},"reference":[{"key":"79481_CR1","doi-asserted-by":"publisher","first-page":"27066","DOI":"10.3402\/jev.v4.27066","volume":"4","author":"M Y\u00e1\u00f1ez-M\u00f3","year":"2015","unstructured":"Y\u00e1\u00f1ez-M\u00f3, M. et al. Biological properties of extracellular vesicles and their physiological functions. J. Extracell. Vesicles 4, 27066 (2015).","journal-title":"J. Extracell. Vesicles"},{"key":"79481_CR2","doi-asserted-by":"publisher","first-page":"1224","DOI":"10.1172\/JCI81137","volume":"126","author":"JM Pitt","year":"2016","unstructured":"Pitt, J. M. et al. Dendritic cell-derived exosomes for cancer therapy. J. Clin. Investig. 126, 1224\u20131232 (2016).","journal-title":"J. Clin. Investig."},{"key":"79481_CR3","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1186\/s13287-019-1484-6","volume":"10","author":"G Qiu","year":"2019","unstructured":"Qiu, G. et al. Functional proteins of mesenchymal stem cell-derived extracellular vesicles. Stem Cell. Res. Ther. 10, 359 (2019).","journal-title":"Stem Cell. Res. Ther."},{"key":"79481_CR4","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1146\/annurev-pharmtox-010814-124630","volume":"55","author":"B Gy\u00f6rgy","year":"2015","unstructured":"Gy\u00f6rgy, B., Hung, M. E., Breakefield, X. O. & Leonard, J. N. Therapeutic applications of extracellular vesicles: clinical promise and open questions. Annu. Rev. Pharmacol. Toxicol. 55, 439\u2013464 (2015).","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"79481_CR5","doi-asserted-by":"publisher","first-page":"129","DOI":"10.3233\/TUB-211543","volume":"44","author":"R Manukonda","year":"2022","unstructured":"Manukonda, R., Attem, J., Yenuganti, V. R., Kaliki, S. & Vemuganti, G. K. Exosomes in the visual system: new avenues in ocular diseases. Tumour Biol. J. Int. Soc. Oncodev. Biol. Med. 44, 129\u2013152 (2022).","journal-title":"Tumour Biol. J. Int. Soc. Oncodev. Biol. Med."},{"key":"79481_CR6","doi-asserted-by":"publisher","first-page":"32945","DOI":"10.3402\/jev.v5.32945","volume":"5","author":"C Gardiner","year":"2016","unstructured":"Gardiner, C. et al. Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. J. Extracell. Vesicles 5, 32945 (2016).","journal-title":"J. Extracell. Vesicles"},{"key":"79481_CR7","doi-asserted-by":"crossref","unstructured":"Konoshenko, M. Y., Lekchnov, E. A., Vlassov, A. V. & Laktionov, P. P. Isolation of extracellular vesicles: General methodologies and latest trends. Biomed. Res. Int. 8545347 (2018).","DOI":"10.1155\/2018\/8545347"},{"key":"79481_CR8","doi-asserted-by":"publisher","first-page":"29509","DOI":"10.3402\/jev.v4.29509","volume":"4","author":"R Linares","year":"2015","unstructured":"Linares, R., Tan, S., Gounou, C., Arraud, N. & Brisson, A. R. High-speed centrifugation induces aggregation of extracellular vesicles. J. Extracell. Vesicles 4, 29509 (2015).","journal-title":"J. Extracell. Vesicles"},{"key":"79481_CR9","doi-asserted-by":"publisher","first-page":"3684","DOI":"10.7150\/thno.41580","volume":"10","author":"D Yang","year":"2020","unstructured":"Yang, D. et al. Progress, opportunity, and perspective on exosome isolation\u2014efforts for efficient exosome-based theranostics. Theranostics 10, 3684\u20133707 (2020).","journal-title":"Theranostics"},{"key":"79481_CR10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3402\/jev.v3.24858","volume":"3","author":"J Van Deun","year":"2014","unstructured":"Van Deun, J. et al. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J. Extracell. Vesicles 3, 1 (2014).","journal-title":"J. Extracell. Vesicles"},{"key":"79481_CR11","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1016\/j.nano.2015.01.003","volume":"11","author":"JZ Nordin","year":"2015","unstructured":"Nordin, J. Z. et al. Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. Nanomedicine 11, 879\u2013883 (2015).","journal-title":"Nanomedicine"},{"key":"79481_CR12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/ijms21186466","volume":"21","author":"K Sidhom","year":"2020","unstructured":"Sidhom, K., Obi, P. O. & Saleem, A. A review of exosomal isolation methods: is size exclusion chromatography the best option? Int. J. Mol. Sci. 21, 1 (2020).","journal-title":"Int. J. Mol. Sci."},{"key":"79481_CR13","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1016\/j.exer.2003.09.003","volume":"78","author":"ME Stern","year":"2004","unstructured":"Stern, M. E., Gao, J., Siemasko, K. F., Beuerman, R. W. & Pflugfelder, S. C. The role of the lacrimal functional unit in the pathophysiology of dry eye. Exp. Eye Res. 78, 409\u2013416 (2004).","journal-title":"Exp. Eye Res."},{"key":"79481_CR14","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.jtos.2013.10.004","volume":"12","author":"I Cher","year":"2014","unstructured":"Cher, I. Ocular surface concepts: development and citation. Ocul. Surf. 12, 10\u201313 (2014).","journal-title":"Ocul. Surf."},{"key":"79481_CR15","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.preteyeres.2016.04.005","volume":"54","author":"IK Gipson","year":"2016","unstructured":"Gipson, I. K. Goblet cells of the conjunctiva: a review of recent findings. Prog. Retin. Eye Res. 54, 49\u201363 (2016).","journal-title":"Prog. Retin. Eye Res."},{"key":"79481_CR16","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.preteyeres.2017.04.004","volume":"59","author":"M Klingeborn","year":"2017","unstructured":"Klingeborn, M., Dismuke, W. M., Rickman, B., Stamer, W. D. & C. & Roles of exosomes in the normal and diseased eye. Prog. Retin. Eye Res. 59, 158\u2013177 (2017).","journal-title":"Prog. Retin. Eye Res."},{"key":"79481_CR17","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1186\/s13287-019-1372-0","volume":"10","author":"N Li","year":"2019","unstructured":"Li, N. et al. Recent advances of exosomes in immune-mediated eye diseases. Stem Cell. Res. Ther. 10, 278 (2019).","journal-title":"Stem Cell. Res. Ther."},{"key":"79481_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/cells9051080","volume":"9","author":"TB McKay","year":"2020","unstructured":"McKay, T. B., Hutcheon, A. E. K., Zieske, J. D. & Ciolino, J. B. Extracellular vesicles secreted by corneal epithelial cells promote myofibroblast differentiation. Cells 9, 1 (2020).","journal-title":"Cells"},{"key":"79481_CR19","doi-asserted-by":"publisher","first-page":"40548","DOI":"10.1038\/srep40548","volume":"7","author":"KY Han","year":"2017","unstructured":"Han, K. Y., Tran, J. A., Chang, J. H., Azar, D. T. & Zieske, J. D. potential role of corneal epithelial cell-derived exosomes in corneal wound healing and neovascularization. Sci. Rep. 7, 40548 (2017).","journal-title":"Sci. Rep."},{"key":"79481_CR20","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1159\/000519458","volume":"65","author":"G Wang","year":"2022","unstructured":"Wang, G. et al. Exosomes derived from mouse adipose-derived mesenchymal stem cells alleviate benzalkonium chloride-induced mouse dry eye model via inhibiting NLRP3 inflammasome. Ophthal. Res. 65, 40\u201351 (2022).","journal-title":"Ophthal. Res."},{"key":"79481_CR21","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1167\/iovs.64.12.38","volume":"64","author":"L Garc\u00eda-Posadas","year":"2023","unstructured":"Garc\u00eda-Posadas, L. et al. Isolation and characterization of human conjunctival mesenchymal stromal cells and their extracellular vesicles. Investig. Ophthalmol. Vis. Sci. 64, 38 (2023).","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"79481_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/ijms23031718","volume":"23","author":"T Ramos","year":"2022","unstructured":"Ramos, T., Parekh, M., Kaye, S. B. & Ahmad, S. Epithelial cell-derived extracellular vesicles trigger the differentiation of two epithelial cell lines. Int. J. Mol. Sci. 23, 1 (2022).","journal-title":"Int. J. Mol. Sci."},{"key":"79481_CR23","doi-asserted-by":"publisher","first-page":"123058","DOI":"10.1016\/j.lfs.2024.123058","volume":"357","author":"C Lee","year":"2024","unstructured":"Lee, C. & Dartt, D. A. Sex-dependent differential increase of specialized pro-resolving mediators in extracellular vesicles secreted by human primary conjunctival goblet cells during allergic inflammation. Life Sci. 357, 123058 (2024).","journal-title":"Life Sci."},{"key":"79481_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/metabo13020187","volume":"13","author":"DY Shu","year":"2023","unstructured":"Shu, D. Y. et al. Role of oxidative stress in ocular diseases: a balancing act. Metabolites 13, 1 (2023).","journal-title":"Metabolites"},{"key":"79481_CR25","doi-asserted-by":"publisher","first-page":"109220","DOI":"10.1016\/j.exer.2022.109220","volume":"223","author":"L Garc\u00eda-Posadas","year":"2022","unstructured":"Garc\u00eda-Posadas, L. et al. Characterization and functional performance of a commercial human conjunctival epithelial cell line. Exp. Eye Res. 223, 109220 (2022).","journal-title":"Exp. Eye Res."},{"key":"79481_CR26","doi-asserted-by":"publisher","first-page":"2160","DOI":"10.1002\/stem.2672","volume":"35","author":"S Galindo","year":"2017","unstructured":"Galindo, S. et al. Therapeutic effect of human adipose tissue-derived mesenchymal stem cells in experimental corneal failure due to limbal stem cell niche damage. Stem Cells 35, 2160\u20132174 (2017).","journal-title":"Stem Cells"},{"key":"79481_CR27","doi-asserted-by":"publisher","DOI":"10.3791\/59254","author":"M Skliar","year":"2019","unstructured":"Skliar, M. & Chernyshev, V. S. Imaging of extracellular vesicles by atomic force microscopy. J. Vis. Exp. https:\/\/doi.org\/10.3791\/59254 (2019).","journal-title":"J. Vis. Exp."},{"key":"79481_CR28","doi-asserted-by":"publisher","first-page":"16701","DOI":"10.1021\/acsomega.0c01582","volume":"5","author":"G Midekessa","year":"2020","unstructured":"Midekessa, G. et al. Zeta potential of extracellular vesicles: toward understanding the attributes that determine colloidal stability. ACS Omega 5, 16701\u201316710 (2020).","journal-title":"ACS Omega"},{"key":"79481_CR29","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1007\/s00281-018-0682-0","volume":"40","author":"EI Buz\u00e1s","year":"2018","unstructured":"Buz\u00e1s, E. I., T\u00f3th, E. \u00c1., S\u00f3dar, B. W. & Szab\u00f3-Taylor, K. \u00c9. Molecular interactions at the surface of extracellular vesicles. Semin. Immunopathol. 40, 453\u2013464 (2018).","journal-title":"Semin. Immunopathol."},{"key":"79481_CR30","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1111\/joa.13365","volume":"238","author":"E Gonz\u00e1lez-Cubero","year":"2021","unstructured":"Gonz\u00e1lez-Cubero, E., Gonz\u00e1lez-Fern\u00e1ndez, M. L., Guti\u00e9rrez-Velasco, L. & Navarro-Ram\u00edrez, E. Villar-Su\u00e1rez, V. isolation and characterization of exosomes from adipose tissue-derived mesenchymal stem cells. J. Anat. 238, 1203\u20131217 (2021).","journal-title":"J. Anat."},{"key":"79481_CR31","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1002\/sctm.20-0376","volume":"10","author":"RMS Cardoso","year":"2021","unstructured":"Cardoso, R. M. S. et al. Development of an optimized and scalable method for isolation of umbilical cord blood-derived small extracellular vesicles for future clinical use. Stem Cells Transl. Med. 10, 910\u2013921 (2021).","journal-title":"Stem Cells Transl. Med."},{"key":"79481_CR32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/mi15010024","volume":"15","author":"J Rodriguez-Loya","year":"2023","unstructured":"Rodriguez-Loya, J., Lerma, M. & Gardea-Torresdey, J. L. Dynamic light scattering and its application to control nanoparticle aggregation in colloidal systems: a review. Micromachines 15, 1 (2023).","journal-title":"Micromachines"},{"key":"79481_CR33","doi-asserted-by":"publisher","first-page":"113097","DOI":"10.1016\/j.yexcr.2022.113097","volume":"414","author":"AG Zhao","year":"2022","unstructured":"Zhao, A. G., Shah, K., Cromer, B. & Sumer, H. Comparative analysis of extracellular vesicles isolated from human mesenchymal stem cells by different isolation methods and visualisation of their uptake. Exp. Cell. Res. 414, 113097 (2022).","journal-title":"Exp. Cell. Res."},{"key":"79481_CR34","doi-asserted-by":"publisher","first-page":"15297","DOI":"10.1038\/s41598-017-15717-7","volume":"7","author":"BJ Benedikter","year":"2017","unstructured":"Benedikter, B. J. et al. Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies. Sci. Rep. 7, 15297 (2017).","journal-title":"Sci. Rep."},{"key":"79481_CR35","doi-asserted-by":"publisher","first-page":"12013","DOI":"10.1088\/1742-6596\/1942\/1\/012013","volume":"1942","author":"D Bagrov","year":"2021","unstructured":"Bagrov, D. et al. Application of AFM, TEM, and NTA for characterization of exosomes produced by placenta-derived mesenchymal cells. J. Phys. Conf. Ser. 1942, 12013 (2021).","journal-title":"J. Phys. Conf. Ser."},{"key":"79481_CR36","doi-asserted-by":"publisher","first-page":"e12152","DOI":"10.1002\/jev2.12152","volume":"10","author":"KS Lee","year":"2021","unstructured":"Lee, K. S. et al. Extracellular vesicles from adipose tissue-derived stem cells alleviate osteoporosis through osteoprotegerin and miR-21-5p. J. Extracell. Vesicles 10, e12152 (2021).","journal-title":"J. Extracell. Vesicles"},{"key":"79481_CR37","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.ab.2012.06.004","volume":"428","author":"C Gercel-Taylor","year":"2012","unstructured":"Gercel-Taylor, C., Atay, S., Tullis, R. H., Kesimer, M. & Taylor, D. D. Nanoparticle analysis of circulating cell-derived vesicles in ovarian cancer patients. Anal. Biochem. 428, 44\u201353 (2012).","journal-title":"Anal. Biochem."},{"key":"79481_CR38","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3402\/jev.v3.24641","volume":"3","author":"LA Mulcahy","year":"2014","unstructured":"Mulcahy, L. A., Pink, R. C. & Carter, D. R. F. Routes and mechanisms of extracellular vesicle uptake. J. Extracell. Vesicles 3, 1 (2014).","journal-title":"J. Extracell. Vesicles"},{"key":"79481_CR39","doi-asserted-by":"publisher","first-page":"204173142311746","DOI":"10.1177\/20417314231174609","volume":"14","author":"S Williams","year":"2023","unstructured":"Williams, S. et al. Comparison of extracellular vesicle isolation processes for therapeutic applications. J. Tissue Eng. 14, 20417314231174610 (2023).","journal-title":"J. Tissue Eng."},{"key":"79481_CR40","doi-asserted-by":"publisher","first-page":"e12404","DOI":"10.1002\/jev2.12404","volume":"13","author":"JA Welsh","year":"2024","unstructured":"Welsh, J. A. et al. Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches. J. Extracell. Vesicles 13, e12404 (2024).","journal-title":"J. Extracell. Vesicles"},{"key":"79481_CR41","doi-asserted-by":"publisher","first-page":"1555410","DOI":"10.1080\/20013078.2018.1555410","volume":"8","author":"J Karttunen","year":"2019","unstructured":"Karttunen, J. et al. Precipitation-based extracellular vesicle isolation from rat plasma co-precipitate vesicle-free microRNAs. J. Extracell. Vesicles 8, 1555410 (2019).","journal-title":"J. Extracell. Vesicles"},{"key":"79481_CR42","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1167\/tvst.7.3.6","volume":"7","author":"Y Zhao","year":"2018","unstructured":"Zhao, Y. et al. Liquid biopsy of vitreous reveals an abundant vesicle population consistent with the size and morphology of exosomes. Transl. Vis. Sci. Technol. 7, 6 (2018).","journal-title":"Transl. Vis. Sci. Technol."},{"key":"79481_CR43","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/s13048-020-0609-y","volume":"13","author":"L Cheng","year":"2020","unstructured":"Cheng, L. et al. Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells. J. Ovarian Res. 13, 9 (2020).","journal-title":"J. Ovarian Res."},{"key":"79481_CR44","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1128\/mbio.02344-17","volume":"9","author":"RP McNamara","year":"2018","unstructured":"McNamara, R. P. et al. Nef secretion into extracellular vesicles or exosomes is conserved across human and simian immunodeficiency viruses. MBio 9, 17. https:\/\/doi.org\/10.1128\/mbio.02344-17 (2018).","journal-title":"MBio"},{"key":"79481_CR45","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1167\/iovs.17-23402","volume":"59","author":"M Dogru","year":"2018","unstructured":"Dogru, M., Kojima, T., Simsek, C. & Tsubota, K. Potential role of oxidative stress in ocular surface inflammation and dry eye disease. Investig. Ophthalmol. Vis. Sci. 59, 163\u2013168 (2018).","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"79481_CR46","doi-asserted-by":"publisher","first-page":"100957","DOI":"10.1016\/j.mtbio.2024.100957","volume":"25","author":"T Yu","year":"2024","unstructured":"Yu, T. et al. Biomimetic nanomaterials in myocardial infarction treatment: harnessing bionic strategies for advanced therapeutics. Mater. Today Bio 25, 100957 (2024).","journal-title":"Mater. Today Bio"},{"key":"79481_CR47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/ijms21176105","volume":"21","author":"N Lerner","year":"2020","unstructured":"Lerner, N., Chen, I., Schreiber-Avissar, S. & Beit-Yannai, E. Extracellular vesicles mediate anti-oxidative response\u2014In vitro study in the ocular drainage system. Int. J. Mol. Sci. 21, 1 (2020).","journal-title":"Int. J. Mol. Sci."},{"key":"79481_CR48","doi-asserted-by":"publisher","first-page":"e13367","DOI":"10.1111\/aji.13367","volume":"85","author":"S Tiwari","year":"2021","unstructured":"Tiwari, S., Kumar, V., Randhawa, S. & Verma, S. K. Preparation and characterization of extracellular vesicles. Am. J. Reprod. Immunol. 85, e13367 (2021).","journal-title":"Am. J. Reprod. Immunol."},{"key":"79481_CR49","doi-asserted-by":"publisher","first-page":"1548","DOI":"10.1038\/s41596-020-00466-1","volume":"16","author":"R Crescitelli","year":"2021","unstructured":"Crescitelli, R., L\u00e4sser, C. & L\u00f6tvall, J. Isolation and characterization of extracellular vesicle subpopulations from tissues. Nat. Protoc. 16, 1548\u20131580 (2021).","journal-title":"Nat. Protoc."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-79481-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-79481-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-79481-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T11:28:22Z","timestamp":1732015702000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-79481-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,16]]},"references-count":49,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["79481"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-79481-1","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,16]]},"assertion":[{"value":"3 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"28270"}}