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These fungicides must be restricted because of their toxicity and the development of resistance strains. Therefore, utilizing natural nanoscale materials in agricultural production is a potential alternative. This work aimed to investigate the antifungal properties of a nanocomposite (nano-chitosan-coated, green-synthesized selenium nanoparticles) against the plant pathogenic fungus <jats:italic>S. sclerotiorum<\/jats:italic>. Chemical reduction was used to produce selenium nanoparticles from citrus peel extracts, and ionotropic gelation was used to produce chitosan nanoparticles. The nanocomposite has been produced using selenium nanoparticles stabilized by chitosan and cross-linked with sodium tripolyphosphate. Transmission electron microscopy, dynamic light scattering, X-ray diffraction, UV-VIS spectroscopy, and Fourier transform infrared spectroscopy were used to characterize all produced nanostructures. The in vitro antifungal activity and minimum inhibitory concentration of all bulk and nanostructures are investigated at (0.5, 1, 5, 10, 50, 100) ppm concentrations. Scanning electron microscopy was used to detect structural deformations in the fungal mycelium. The findings support the successful synthesis and characterization of all nanoparticles. Lemon peel extract produced smaller, more stable, and distributed selenium nanoparticles (42.28\u2009\u00b1\u200918.5\u00a0nm) than orange peel extract (85.7\u2009\u00b1\u2009140.22\u00a0nm). Nanostructures, particularly nanocomposite, have shown a considerable increase in antifungal efficacy compared to bulk structures. At a minimum inhibitory concentration of 0.5 ppm, the nanocomposite exhibited 100% inhibitory activity. The nanocomposite with a concentration of 0.5 ppm exhibited the lowest average fungal biomass (0.32\u2009\u00b1\u20090.05\u00a0g) among all tested nanostructures. Fungal hyphae treated with 0.5 ppm of nanocomposite within 18\u00a0h of treatment revealed substantial damage and deformation. These results provide new insights into the nanocomposite as an eco-friendly and promising antifungal agent against other plant pathogenic fungi.<\/jats:p>","DOI":"10.1038\/s41598-024-79574-x","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T15:22:14Z","timestamp":1736176934000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Nano-chitosan-coated, green-synthesized selenium nanoparticles as a novel antifungal agent against Sclerotinia sclerotiorum: in vitro study"],"prefix":"10.1038","volume":"15","author":[{"given":"Mohamed M.","family":"Desouky","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radwa H.","family":"Abou-Saleh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek A. 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