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In this study, 170 samples of total bacteria, total coliform, and total fungi were collected from 10 sites within a domestic WWTP, Alexandria, Egypt, using the sedimentation technique. According to the Index of Microbial Air Contamination (IMA) classes, the total bacteria range was 108\u20135120 CFU\/dm<jats:sup>2<\/jats:sup>\/hour, and all samples were classified as \u201cvery poor\u201d except one sample of an office, which was classified as \u201cpoor.\u201d The total coliform range was 0\u2013565 CFU\/dm<jats:sup>2<\/jats:sup>\/hour, and 6 samples were classified as \u201cvery poor,\u201d while one sample was classified as \u201cpoor.\u201d The total fungi range was 0\u2013209 CFU\/dm<jats:sup>2<\/jats:sup>\/hour, and 9 samples were classified as \u201cvery poor,\u201d while 4 samples were classified as \u201cpoor.\u201d After the conversion to CFU\/m<jats:sup>3<\/jats:sup>, the counts of total bacteria, total coliforms, and total fungi were 897\u2009\u2212\u200942.7\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>, 0\u20134.71\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>, and 0\u20132.69\u2009\u00d7\u200910<jats:sup>3<\/jats:sup> CFU\/m<jats:sup>3<\/jats:sup>, respectively. Several identified bioaerosols have been reported before as a cause of human infections. They included <jats:italic>Lysinibacillus fusiformis<\/jats:italic>,<jats:italic> Bacillus cereus<\/jats:italic>,<jats:italic> Alcaligenes faecalis<\/jats:italic>,<jats:italic> Klebsiella<\/jats:italic> sp., <jats:italic>Escherichia coli<\/jats:italic>,<jats:italic> Aspergillus<\/jats:italic> spp., <jats:italic>Penicillium<\/jats:italic> spp., <jats:italic>Rhizopus<\/jats:italic> sp., <jats:italic>Candida<\/jats:italic> sp., and <jats:italic>Rhodotorula<\/jats:italic> sp. These results indicated an increased health risk to WWTP staff, which needs more attention and more efficient control measures.<\/jats:p>","DOI":"10.1038\/s41598-024-79645-z","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T12:55:54Z","timestamp":1734353754000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Quantitative and qualitative analysis of bioaerosols emissions from the domestic eastern wastewater treatment plant, Alexandria, Egypt"],"prefix":"10.1038","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5351-9696","authenticated-orcid":false,"given":"Ebtesam","family":"El-Bestawy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Mahmoud","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El sayed Ahmed","family":"Shalaby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"79645_CR1","unstructured":"World Health Organization (WHO). 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