{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T19:24:45Z","timestamp":1793733885990,"version":"4.6.1"},"reference-count":85,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R21AR080388-02"],"award-info":[{"award-number":["5R21AR080388-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R21AR080388-02"],"award-info":[{"award-number":["5R21AR080388-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R21AR080388-02"],"award-info":[{"award-number":["5R21AR080388-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R21AR080388-02"],"award-info":[{"award-number":["5R21AR080388-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-80050-9","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T07:00:59Z","timestamp":1732690859000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The roles of regulatory-compliant media and inflammatory\/oxytocin priming selection in enhancing human mesenchymal stem\/stromal cell immunomodulatory properties"],"prefix":"10.1038","volume":"14","author":[{"given":"Mahammad","family":"Gardashli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Baron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Drohat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Quintero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee D.","family":"Kaplan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Szeto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando J.","family":"Mendez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Best","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Kouroupis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,27]]},"reference":[{"issue":"11","key":"80050_CR1","doi-asserted-by":"publisher","first-page":"166","DOI":"10.3390\/bioengineering8110166","volume":"8","author":"D Kouroupis","year":"2021","unstructured":"Kouroupis, D., Best, T. M., Kaplan, L. D., Correa, D. & Griswold, A. J. Single-cell RNA-sequencing identifies infrapatellar fat pad macrophage polarization in acute synovitis\/fat pad fibrosis and cell therapy. Bioengineering 8(11), 166. https:\/\/doi.org\/10.3390\/bioengineering8110166 (2021).","journal-title":"Bioengineering"},{"key":"80050_CR2","doi-asserted-by":"publisher","first-page":"2013","DOI":"10.1177\/0363546520917699","volume":"48","author":"D Kouroupis","year":"2020","unstructured":"Kouroupis, D., Bowles, A. C., Best, T. M., Kaplan, L. D. & Correa, D. CD10\/Neprilysin enrichment in infrapatellar fat pad-derived mesenchymal stem cells under regulatory-compliant conditions: Implications for efficient synovitis and fat pad fibrosis reversal. Am. J. Sports Med. 48, 2013\u20132027. https:\/\/doi.org\/10.1177\/0363546520917699 (2020).","journal-title":"Am. J. Sports Med."},{"key":"80050_CR3","doi-asserted-by":"publisher","first-page":"953510","DOI":"10.1155\/2012\/953510","volume":"2012","author":"C Pascual-Garrido","year":"2012","unstructured":"Pascual-Garrido, C., Rol\u00f3n, A. & Makino, A. Treatment of chronic patellar tendinopathy with autologous bone marrow stem cells: A 5-year-followup. Stem Cells Int. 2012, 953510. https:\/\/doi.org\/10.1155\/2012\/953510 (2012).","journal-title":"Stem Cells Int."},{"key":"80050_CR4","doi-asserted-by":"publisher","first-page":"iv43","DOI":"10.1093\/rheumatology\/kex419","volume":"57","author":"K Fu","year":"2018","unstructured":"Fu, K., Robbins, S. R. & McDougall, J. J. Osteoarthritis: The genesis of pain. Rheumatology 57, iv43\u2013iv50. https:\/\/doi.org\/10.1093\/rheumatology\/kex419 (2018).","journal-title":"Rheumatology"},{"key":"80050_CR5","first-page":"448","volume":"13","author":"C Zhu","year":"2021","unstructured":"Zhu, C., Wu, W. & Qu, X. Mesenchymal stem cells in osteoarthritis therapy: A review. Am. J. Transl. Res. 13, 448\u2013461 (2021).","journal-title":"Am. J. Transl. Res."},{"key":"80050_CR6","doi-asserted-by":"publisher","first-page":"2020","DOI":"10.1016\/j.arthro.2013.09.074","volume":"29","author":"KL Wong","year":"2013","unstructured":"Wong, K. L. et al. Injectable cultured bone marrow-derived mesenchymal stem cells in varus knees with cartilage defects undergoing high tibial osteotomy: A prospective, randomized controlled clinical trial with 2 years\u2019 follow-up. Arthroscopy 29, 2020\u20132028. https:\/\/doi.org\/10.1016\/j.arthro.2013.09.074 (2013).","journal-title":"Arthroscopy"},{"key":"80050_CR7","doi-asserted-by":"publisher","first-page":"90","DOI":"10.2106\/JBJS.M.00058","volume":"96","author":"CT Vangsness Jr","year":"2014","unstructured":"Vangsness, C. T. Jr. et al. Adult human mesenchymal stem cells delivered via intra-articular injection to the knee following partial medial meniscectomy: A randomized, double-blind, controlled study. J. Bone Joint Surg. Am. 96, 90\u201398. https:\/\/doi.org\/10.2106\/JBJS.M.00058 (2014).","journal-title":"J. Bone Joint Surg. Am."},{"key":"80050_CR8","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1186\/s12967-016-0998-2","volume":"14","author":"JM Lamo-Espinosa","year":"2016","unstructured":"Lamo-Espinosa, J. M. et al. Intra-articular injection of two different doses of autologous bone marrow mesenchymal stem cells versus hyaluronic acid in the treatment of knee osteoarthritis: Multicenter randomized controlled clinical trial (phase I\/II). J. Transl. Med. 14, 246. https:\/\/doi.org\/10.1186\/s12967-016-0998-2 (2016).","journal-title":"J. Transl. Med."},{"key":"80050_CR9","doi-asserted-by":"publisher","first-page":"847","DOI":"10.5966\/sctm.2015-0245","volume":"5","author":"YM Pers","year":"2016","unstructured":"Pers, Y. M. et al. Adipose mesenchymal stromal cell-based therapy for severe osteoarthritis of the knee: A phase I dose-escalation trial. Stem Cells Transl. Med. 5, 847\u2013856. https:\/\/doi.org\/10.5966\/sctm.2015-0245 (2016).","journal-title":"Stem Cells Transl. Med."},{"key":"80050_CR10","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.arthro.2015.09.010","volume":"32","author":"YG Koh","year":"2016","unstructured":"Koh, Y. G., Kwon, O. R., Kim, Y. S., Choi, Y. J. & Tak, D. H. Adipose-derived mesenchymal stem cells with microfracture versus microfracture alone: 2-year follow-up of a prospective randomized trial. Arthroscopy 32, 97\u2013109. https:\/\/doi.org\/10.1016\/j.arthro.2015.09.010 (2016).","journal-title":"Arthroscopy"},{"key":"80050_CR11","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1002\/sctm.18-0122","volume":"8","author":"WS Lee","year":"2019","unstructured":"Lee, W. S., Kim, H. J., Kim, K. I., Kim, G. B. & Jin, W. Intra-articular injection of autologous adipose tissue-derived mesenchymal stem cells for the treatment of knee osteoarthritis: A phase IIB, randomized, placebo-controlled clinical trial. Stem Cells Transl. Med. 8, 504\u2013511. https:\/\/doi.org\/10.1002\/sctm.18-0122 (2019).","journal-title":"Stem Cells Transl. Med."},{"key":"80050_CR12","doi-asserted-by":"publisher","first-page":"1472","DOI":"10.7507\/1002-1892.20160305","volume":"30","author":"Y Wang","year":"2016","unstructured":"Wang, Y. et al. Curative effect of human umbilical cord mesenchymal stem cells by intra-articular injection for degenerative knee osteoarthritis. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 30, 1472\u20131477. https:\/\/doi.org\/10.7507\/1002-1892.20160305 (2016).","journal-title":"Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi"},{"key":"80050_CR13","doi-asserted-by":"publisher","first-page":"e10088","DOI":"10.1371\/journal.pone.0010088","volume":"5","author":"RS Waterman","year":"2010","unstructured":"Waterman, R. S., Tomchuck, S. L., Henkle, S. L. & Betancourt, A. M. A new mesenchymal stem cell (MSC) paradigm: polarization into a pro-inflammatory MSC1 or an Immunosuppressive MSC2 phenotype. PLoS One 5, e10088. https:\/\/doi.org\/10.1371\/journal.pone.0010088 (2010).","journal-title":"PLoS One"},{"key":"80050_CR14","doi-asserted-by":"publisher","first-page":"1040762","DOI":"10.3389\/fbioe.2023.1040762","volume":"11","author":"D Quintero","year":"2023","unstructured":"Quintero, D. et al. The roles and therapeutic potential of mesenchymal stem\/stromal cells and their extracellular vesicles in tendinopathies. Front. Bioeng. Biotechnol. 11, 1040762. https:\/\/doi.org\/10.3389\/fbioe.2023.1040762 (2023).","journal-title":"Front. Bioeng. Biotechnol."},{"key":"80050_CR15","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1186\/s13287-021-02294-w","volume":"12","author":"D Kouroupis","year":"2021","unstructured":"Kouroupis, D., Willman, M. A., Best, T. M., Kaplan, L. D. & Correa, D. Correction to: Infrapatellar fat pad-derived mesenchymal stem cell-based spheroids enhance their therapeutic efficacy to reverse synovitis and fat pad fibrosis. Stem Cell Res. Ther. 12, 282. https:\/\/doi.org\/10.1186\/s13287-021-02294-w (2021).","journal-title":"Stem Cell Res. Ther."},{"key":"80050_CR16","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1007\/s00167-011-1607-9","volume":"20","author":"JL Ellera Gomes","year":"2012","unstructured":"Ellera Gomes, J. L., da Silva, R. C., Silla, L. M., Abreu, M. R. & Pellanda, R. Conventional rotator cuff repair complemented by the aid of mononuclear autologous stem cells. Knee Surg. Sports Traumatol. Arthrosc. 20, 373\u2013377. https:\/\/doi.org\/10.1007\/s00167-011-1607-9 (2012).","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"80050_CR17","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1038\/nbt.2971","volume":"32","author":"AM Bailey","year":"2014","unstructured":"Bailey, A. M., Mendicino, M. & Au, P. An FDA perspective on preclinical development of cell-based regenerative medicine products. Nat. Biotechnol. 32, 721\u2013723. https:\/\/doi.org\/10.1038\/nbt.2971 (2014).","journal-title":"Nat. Biotechnol."},{"key":"80050_CR18","doi-asserted-by":"crossref","unstructured":"Kouroupis, D., Wang, X. N., El-Sherbiny, Y., McGonagle, D. & Jones, E. in Safety, Ethics and Regulations (eds Phuc Van Pham & Achim Rosemann) 91\u2013118 (Springer International Publishing, 2017).","DOI":"10.1007\/978-3-319-59165-0_4"},{"key":"80050_CR19","doi-asserted-by":"publisher","first-page":"115","DOI":"10.3389\/fcell.2016.00115","volume":"4","author":"C Dessels","year":"2016","unstructured":"Dessels, C., Potgieter, M. & Pepper, M. S. Making the switch: Alternatives to fetal bovine serum for adipose-derived stromal cell expansion. Front. Cell Dev. Biol. 4, 115. https:\/\/doi.org\/10.3389\/fcell.2016.00115 (2016).","journal-title":"Front. Cell Dev. Biol."},{"key":"80050_CR20","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.jcyt.2016.11.011","volume":"19","author":"O Karnieli","year":"2017","unstructured":"Karnieli, O. et al. A consensus introduction to serum replacements and serum-free media for cellular therapies. Cytotherapy 19, 155\u2013169. https:\/\/doi.org\/10.1016\/j.jcyt.2016.11.011 (2017).","journal-title":"Cytotherapy"},{"key":"80050_CR21","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1177\/026119290203000208","volume":"30","author":"CE Jochems","year":"2002","unstructured":"Jochems, C. E., van der Valk, J. B., Stafleu, F. R. & Baumans, V. The use of fetal bovine serum: Ethical or scientific problem?. Altern. Lab Anim. 30, 219\u2013227. https:\/\/doi.org\/10.1177\/026119290203000208 (2002).","journal-title":"Altern. Lab Anim."},{"key":"80050_CR22","doi-asserted-by":"publisher","first-page":"2013","DOI":"10.1177\/0363546520917699","volume":"40","author":"D Kouroupis","year":"2020","unstructured":"Kouroupis, D., Bowles, A. C., Best, T. M., Kaplan, L. D. & Correa, D. CD10\/neprilysin enrichment in infrapatellar fat pad-derived MSC under regulatory-compliant conditions: Implications for efficient synovitis and fat pad fibrosis reversal. Am. J. Sports Med. 40, 2013\u20132027 (2020).","journal-title":"Am. J. Sports Med."},{"key":"80050_CR23","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/j.jcyt.2020.06.007","volume":"22","author":"D Kouroupis","year":"2020","unstructured":"Kouroupis, D. et al. Regulatory-compliant conditions during cell product manufacturing enhance in vitro immunomodulatory properties of infrapatellar fat pad-derived mesenchymal stem\/stromal cells. Cytotherapy 22, 677\u2013689. https:\/\/doi.org\/10.1016\/j.jcyt.2020.06.007 (2020).","journal-title":"Cytotherapy"},{"key":"80050_CR24","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/j.jcyt.2013.11.004","volume":"16","author":"H Hemeda","year":"2014","unstructured":"Hemeda, H., Giebel, B. & Wagner, W. Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells. Cytotherapy 16, 170\u2013180. https:\/\/doi.org\/10.1016\/j.jcyt.2013.11.004 (2014).","journal-title":"Cytotherapy"},{"key":"80050_CR25","doi-asserted-by":"publisher","first-page":"10864","DOI":"10.1038\/s41598-019-47391-2","volume":"9","author":"D Kouroupis","year":"2019","unstructured":"Kouroupis, D. et al. Infrapatellar fat pad-derived MSC response to inflammation and fibrosis induces an immunomodulatory phenotype involving CD10-mediated Substance P degradation. Sci. Rep. 9, 10864. https:\/\/doi.org\/10.1038\/s41598-019-47391-2 (2019).","journal-title":"Sci. Rep."},{"key":"80050_CR26","doi-asserted-by":"publisher","first-page":"48","DOI":"10.3389\/fnins.2013.00048","volume":"7","author":"DS Quintana","year":"2013","unstructured":"Quintana, D. S., Kemp, A. H., Alvares, G. A. & Guastella, A. J. A role for autonomic cardiac control in the effects of oxytocin on social behavior and psychiatric illness. Front. Neurosci. 7, 48. https:\/\/doi.org\/10.3389\/fnins.2013.00048 (2013).","journal-title":"Front. Neurosci."},{"key":"80050_CR27","doi-asserted-by":"publisher","first-page":"109597","DOI":"10.1016\/j.mehy.2020.109597","volume":"138","author":"B Buemann","year":"2020","unstructured":"Buemann, B. & Uvnas-Moberg, K. Oxytocin may have a therapeutical potential against cardiovascular disease. Possible pharmaceutical and behavioral approaches. Med. Hypotheses 138, 109597. https:\/\/doi.org\/10.1016\/j.mehy.2020.109597 (2020).","journal-title":"Med. Hypotheses"},{"key":"80050_CR28","doi-asserted-by":"publisher","first-page":"693","DOI":"10.3389\/fimmu.2016.00693","volume":"7","author":"T Li","year":"2016","unstructured":"Li, T., Wang, P., Wang, S. C. & Wang, Y. F. Approaches mediating oxytocin regulation of the immune system. Front. Immunol. 7, 693. https:\/\/doi.org\/10.3389\/fimmu.2016.00693 (2016).","journal-title":"Front. Immunol."},{"key":"80050_CR29","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.pneurobio.2009.04.001","volume":"88","author":"HJ Lee","year":"2009","unstructured":"Lee, H. J., Macbeth, A. H., Pagani, J. H. & Young, W. S. 3rd. Oxytocin: The great facilitator of life. Prog. Neurobiol. 88, 127\u2013151. https:\/\/doi.org\/10.1016\/j.pneurobio.2009.04.001 (2009).","journal-title":"Prog. Neurobiol."},{"key":"80050_CR30","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1124\/pr.120.019398","volume":"72","author":"CS Carter","year":"2020","unstructured":"Carter, C. S. et al. Is oxytocin \u201cnature\u2019s medicine\u201d?. Pharmacol. Rev. 72, 829\u2013861. https:\/\/doi.org\/10.1124\/pr.120.019398 (2020).","journal-title":"Pharmacol. Rev."},{"key":"80050_CR31","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1016\/j.bbrc.2017.02.115","volume":"486","author":"Y Wu","year":"2017","unstructured":"Wu, Y. et al. Oxytocin prevents cartilage matrix destruction via regulating matrix metalloproteinases. Biochem. Biophys. Res. Commun. 486, 601\u2013606. https:\/\/doi.org\/10.1016\/j.bbrc.2017.02.115 (2017).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"80050_CR32","first-page":"131","volume":"41","author":"SK Elabd","year":"2007","unstructured":"Elabd, S. K., Sabry, I., Hassan, W. B., Nour, H. & Zaky, K. Possible neuroendocrine role for oxytocin in bone remodeling. Endocr. Regul. 41, 131\u2013141 (2007).","journal-title":"Endocr. Regul."},{"key":"80050_CR33","doi-asserted-by":"publisher","first-page":"5361","DOI":"10.1210\/en.2012-1402","volume":"153","author":"N Noiseux","year":"2012","unstructured":"Noiseux, N. et al. Preconditioning of stem cells by oxytocin to improve their therapeutic potential. Endocrinology 153, 5361\u20135372. https:\/\/doi.org\/10.1210\/en.2012-1402 (2012).","journal-title":"Endocrinology"},{"key":"80050_CR34","doi-asserted-by":"publisher","first-page":"5825","DOI":"10.1002\/jcb.27868","volume":"120","author":"MF Taha","year":"2019","unstructured":"Taha, M. F., Javeri, A., Karimipour, M. & Yamaghani, M. S. Priming with oxytocin and relaxin improves cardiac differentiation of adipose tissue-derived stem cells. J. Cell Biochem. 120, 5825\u20135834. https:\/\/doi.org\/10.1002\/jcb.27868 (2019).","journal-title":"J. Cell Biochem."},{"key":"80050_CR35","doi-asserted-by":"publisher","first-page":"1305949","DOI":"10.3389\/fnmol.2023.1305949","volume":"16","author":"K Prescott","year":"2023","unstructured":"Prescott, K. et al. Blocking of microglia-astrocyte proinflammatory signaling is beneficial following stroke. Front. Mol. Neurosci. 16, 1305949. https:\/\/doi.org\/10.3389\/fnmol.2023.1305949 (2023).","journal-title":"Front. Mol. Neurosci."},{"key":"80050_CR36","doi-asserted-by":"publisher","first-page":"4002","DOI":"10.3390\/cells11244002","volume":"11","author":"C Le\u00f1ero","year":"2022","unstructured":"Le\u00f1ero, C., Kaplan, L. D., Best, T. M. & Kouroupis, D. CD146+ endometrial-derived mesenchymal stem\/stromal cell subpopulation possesses exosomal secretomes with strong immunomodulatory miRNA attributes. Cells 11, 4002. https:\/\/doi.org\/10.3390\/cells11244002 (2022).","journal-title":"Cells"},{"key":"80050_CR37","doi-asserted-by":"publisher","first-page":"3609","DOI":"10.1038\/s41598-022-07569-7","volume":"12","author":"D Kouroupis","year":"2022","unstructured":"Kouroupis, D., Kaplan, L. D. & Best, T. M. Human infrapatellar fat pad mesenchymal stem cells show immunomodulatory exosomal signatures. Sci. Rep. 12, 3609. https:\/\/doi.org\/10.1038\/s41598-022-07569-7 (2022).","journal-title":"Sci. Rep."},{"key":"80050_CR38","doi-asserted-by":"publisher","first-page":"484","DOI":"10.3390\/cells13060484","volume":"13","author":"K Liebmann","year":"2024","unstructured":"Liebmann, K. et al. Modification of mesenchymal stem\/stromal cell-derived small extracellular vesicles by calcitonin gene related peptide (CGRP) antagonist: Potential implications for inflammation and pain reversal. Cells 13, 484. https:\/\/doi.org\/10.3390\/cells13060484 (2024).","journal-title":"Cells"},{"key":"80050_CR39","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1016\/j.ymthe.2004.02.012","volume":"9","author":"JL Spees","year":"2004","unstructured":"Spees, J. L. et al. Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cell and gene therapy. Mol. Therapy 9, 747\u2013756. https:\/\/doi.org\/10.1016\/j.ymthe.2004.02.012 (2004).","journal-title":"Mol. Therapy"},{"key":"80050_CR40","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1007\/s00109-016-1471-7","volume":"95","author":"S Bobis-Wozowicz","year":"2017","unstructured":"Bobis-Wozowicz, S. et al. Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles. J. Mol. Med. 95, 205\u2013220. https:\/\/doi.org\/10.1007\/s00109-016-1471-7 (2017).","journal-title":"J. Mol. Med."},{"key":"80050_CR41","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1111\/trf.13902","volume":"57","author":"S Gerby","year":"2017","unstructured":"Gerby, S., Attebi, E., Vlaski, M. & Ivanovic, Z. A new clinical-scale serum-free xeno-free medium efficient in ex vivo amplification of mesenchymal stromal cells does not support mesenchymal stem cells. Transfusion 57, 433\u2013439. https:\/\/doi.org\/10.1111\/trf.13902 (2017).","journal-title":"Transfusion"},{"key":"80050_CR42","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/j.biomaterials.2015.10.065","volume":"76","author":"T Burnouf","year":"2016","unstructured":"Burnouf, T., Strunk, D., Koh, M. B. C. & Schallmoser, K. Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation?. Biomaterials 76, 371\u2013387. https:\/\/doi.org\/10.1016\/j.biomaterials.2015.10.065 (2016).","journal-title":"Biomaterials"},{"key":"80050_CR43","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1186\/s13287-022-02815-1","volume":"13","author":"S Palombella","year":"2022","unstructured":"Palombella, S. et al. Systematic review and meta-analysis on the use of human platelet lysate for mesenchymal stem cell cultures: Comparison with fetal bovine serum and considerations on the production protocol. Stem Cell Res. Ther. 13, 142. https:\/\/doi.org\/10.1186\/s13287-022-02815-1 (2022).","journal-title":"Stem Cell Res. Ther."},{"key":"80050_CR44","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/s13770-020-00306-z","volume":"18","author":"HTH Bui","year":"2021","unstructured":"Bui, H. T. H., Nguyen, L. T. & Than, U. T. T. Influences of xeno-free media on mesenchymal stem cell expansion for clinical application. Tissue Eng. Regen. Med. 18, 15\u201323. https:\/\/doi.org\/10.1007\/s13770-020-00306-z (2021).","journal-title":"Tissue Eng. Regen. Med."},{"key":"80050_CR45","doi-asserted-by":"publisher","first-page":"1954","DOI":"10.1093\/rheumatology\/kev228","volume":"54","author":"DFG Remst","year":"2015","unstructured":"Remst, D. F. G., Blaney Davidson, E. N. & van der Kraan, P. M. Unravelling osteoarthritis-related synovial fibrosis a step closer to solving joint stiffness. Rheumatology 54, 1954\u20131963. https:\/\/doi.org\/10.1093\/rheumatology\/kev228 (2015).","journal-title":"Rheumatology"},{"key":"80050_CR46","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1177\/1759720X12467868","volume":"5","author":"J Sokolove","year":"2013","unstructured":"Sokolove, J. & Lepus, C. M. Role of inflammation in the pathogenesis of osteoarthritis: Latest findings and interpretations. Ther. Adv. Musculoskelet. Dis. 5, 77\u201394. https:\/\/doi.org\/10.1177\/1759720X12467868 (2013).","journal-title":"Ther. Adv. Musculoskelet. Dis."},{"key":"80050_CR47","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1002\/stem.3196","volume":"38","author":"AC Bowles","year":"2020","unstructured":"Bowles, A. C. et al. Signature quality attributes of CD146(+) mesenchymal stem\/stromal cells correlate with high therapeutic and secretory potency. Stem Cells 38, 1034\u20131049. https:\/\/doi.org\/10.1002\/stem.3196 (2020).","journal-title":"Stem Cells"},{"key":"80050_CR48","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s12015-023-10647-6","volume":"20","author":"AP Moreno-Londo\u00f1o","year":"2024","unstructured":"Moreno-Londo\u00f1o, A. P. & Robles-Flores, M. Functional roles of CD133: more than stemness associated factor regulated by the microenvironment. Stem Cell Rev. Rep. 20, 25\u201351. https:\/\/doi.org\/10.1007\/s12015-023-10647-6 (2024).","journal-title":"Stem Cell Rev. Rep."},{"key":"80050_CR49","doi-asserted-by":"publisher","first-page":"280","DOI":"10.3390\/cells9020280","volume":"9","author":"C Aluganti Narasimhulu","year":"2020","unstructured":"Aluganti Narasimhulu, C. & Singla, D. K. The role of bone morphogenetic protein 7 (BMP-7) in inflammation in heart diseases. Cells 9, 280. https:\/\/doi.org\/10.3390\/cells9020280 (2020).","journal-title":"Cells"},{"key":"80050_CR50","doi-asserted-by":"publisher","first-page":"1381","DOI":"10.3892\/etm.2018.6339","volume":"16","author":"X Yan","year":"2018","unstructured":"Yan, X. et al. BMP7-overexpressing bone marrow-derived mesenchymal stem cells (BMSCs) are more effective than wild-type BMSCs in healing fractures. Exp. Ther. Med. 16, 1381\u20131388. https:\/\/doi.org\/10.3892\/etm.2018.6339 (2018).","journal-title":"Exp. Ther. Med."},{"key":"80050_CR51","doi-asserted-by":"publisher","first-page":"4165","DOI":"10.1016\/j.injury.2022.09.017","volume":"53","author":"M Kumlin","year":"2022","unstructured":"Kumlin, M., Lindberg, K., Haldosen, L. A., Fellander-Tsai, L. & Li, Y. Growth differentiation factor 7 promotes multiple-lineage differentiation in tenogenic cultures of mesenchymal stem cells. Injury 53, 4165\u20134168. https:\/\/doi.org\/10.1016\/j.injury.2022.09.017 (2022).","journal-title":"Injury"},{"key":"80050_CR52","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1615\/critrevimmunol.v32.i1.30","volume":"32","author":"SS Iyer","year":"2012","unstructured":"Iyer, S. S. & Cheng, G. Role of interleukin 10 transcriptional regulation in inflammation and autoimmune disease. Crit. Rev. Immunol. 32, 23\u201363. https:\/\/doi.org\/10.1615\/critrevimmunol.v32.i1.30 (2012).","journal-title":"Crit. Rev. Immunol."},{"key":"80050_CR53","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1186\/s13075-021-02662-0","volume":"23","author":"C Wen","year":"2021","unstructured":"Wen, C. et al. Insulin-like growth factor-1 in articular cartilage repair for osteoarthritis treatment. Arthritis Res. Ther. 23, 277. https:\/\/doi.org\/10.1186\/s13075-021-02662-0 (2021).","journal-title":"Arthritis Res. Ther."},{"key":"80050_CR54","doi-asserted-by":"publisher","first-page":"194760352210981","DOI":"10.1177\/19476035221098167","volume":"13","author":"EM van Helvoort","year":"2022","unstructured":"van Helvoort, E. M., van der Heijden, E., van Roon, J. A. G., Eijkelkamp, N. & Mastbergen, S. C. The role of interleukin-4 and interleukin-10 in osteoarthritic joint disease: A systematic narrative review. Cartilage 13, 19476035221098170. https:\/\/doi.org\/10.1177\/19476035221098167 (2022).","journal-title":"Cartilage"},{"key":"80050_CR55","doi-asserted-by":"publisher","first-page":"3555","DOI":"10.2147\/JIR.S316756","volume":"14","author":"MA Hossain","year":"2021","unstructured":"Hossain, M. A. et al. IGF-1 facilitates cartilage reconstruction by regulating PI3K\/AKT, MAPK, and NF-kB signaling in rabbit osteoarthritis. J. Inflamm. Res. 14, 3555\u20133568. https:\/\/doi.org\/10.2147\/JIR.S316756 (2021).","journal-title":"J. Inflamm. Res."},{"key":"80050_CR56","doi-asserted-by":"publisher","first-page":"1606","DOI":"10.1359\/jbmr.1997.12.10.1606","volume":"12","author":"K Hanada","year":"1997","unstructured":"Hanada, K., Dennis, J. E. & Caplan, A. I. Stimulatory effects of basic fibroblast growth factor and bone morphogenetic protein-2 on osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. J. Bone Miner. Res. 12, 1606\u20131614. https:\/\/doi.org\/10.1359\/jbmr.1997.12.10.1606 (1997).","journal-title":"J. Bone Miner. Res."},{"key":"80050_CR57","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1007\/s002239900581","volume":"64","author":"A Scutt","year":"1999","unstructured":"Scutt, A. & Bertram, P. Basic fibroblast growth factor in the presence of dexamethasone stimulates colony formation, expansion, and osteoblastic differentiation by rat bone marrow stromal cells. Calcif. Tissue Int. 64, 69\u201377. https:\/\/doi.org\/10.1007\/s002239900581 (1999).","journal-title":"Calcif. Tissue Int."},{"key":"80050_CR58","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.3892\/mmr.2013.1668","volume":"8","author":"TY Cai","year":"2013","unstructured":"Cai, T. Y. et al. Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway. Mol. Med. Rep. 8, 1323\u20131328. https:\/\/doi.org\/10.3892\/mmr.2013.1668 (2013).","journal-title":"Mol. Med. Rep."},{"key":"80050_CR59","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1111\/cpr.12046","volume":"46","author":"S Hagmann","year":"2013","unstructured":"Hagmann, S. et al. FGF-2 addition during expansion of human bone marrow-derived stromal cells alters MSC surface marker distribution and chondrogenic differentiation potential. Cell Prolif. 46, 396\u2013407. https:\/\/doi.org\/10.1111\/cpr.12046 (2013).","journal-title":"Cell Prolif."},{"key":"80050_CR60","doi-asserted-by":"publisher","first-page":"18545","DOI":"10.1038\/s41598-020-75584-7","volume":"10","author":"Y Koike","year":"2020","unstructured":"Koike, Y., Yozaki, M., Utani, A. & Murota, H. Fibroblast growth factor 2 accelerates the epithelial-mesenchymal transition in keratinocytes during wound healing process. Sci. Rep. 10, 18545. https:\/\/doi.org\/10.1038\/s41598-020-75584-7 (2020).","journal-title":"Sci. Rep."},{"key":"80050_CR61","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1089\/ten.TEA.2008.0551","volume":"16","author":"CA Hellingman","year":"2010","unstructured":"Hellingman, C. A. et al. Fibroblast growth factor receptors in in vitro and in vivo chondrogenesis: Relating tissue engineering using adult mesenchymal stem cells to embryonic development. Tissue Eng. Part A 16, 545\u2013556. https:\/\/doi.org\/10.1089\/ten.TEA.2008.0551 (2010).","journal-title":"Tissue Eng. Part A"},{"key":"80050_CR62","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1159\/000499724","volume":"47","author":"EM Matzhold","year":"2020","unstructured":"Matzhold, E. M., Wagner, T., Drexler, C., Schonbacher, M. & Kormoczi, G. F. Aberrant ABO B phenotype with irregular anti-B caused by a para-Bombay FUT1 mutation. Transfus. Med. Hemother. 47, 94\u201397. https:\/\/doi.org\/10.1159\/000499724 (2020).","journal-title":"Transfus. Med. Hemother."},{"key":"80050_CR63","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1111\/imm.13716","volume":"171","author":"JH Ko","year":"2024","unstructured":"Ko, J. H., Ryu, J. S., Oh, J. H. & Oh, J. Y. Splenocytes with fucosylation deficiency promote T cell proliferation and differentiation through thrombospondin-1 downregulation. Immunology 171, 262\u2013269. https:\/\/doi.org\/10.1111\/imm.13716 (2024).","journal-title":"Immunology"},{"key":"80050_CR64","doi-asserted-by":"publisher","first-page":"5067","DOI":"10.1182\/blood-2010-08-304287","volume":"117","author":"A Tormin","year":"2011","unstructured":"Tormin, A. et al. CD146 expression on primary nonhematopoietic bone marrow stem cells is correlated with in situ localization. Blood 117, 5067\u20135077. https:\/\/doi.org\/10.1182\/blood-2010-08-304287 (2011).","journal-title":"Blood"},{"key":"80050_CR65","doi-asserted-by":"publisher","first-page":"2891","DOI":"10.1182\/blood-2012-08-451864","volume":"121","author":"M Corselli","year":"2013","unstructured":"Corselli, M. et al. Perivascular support of human hematopoietic stem\/progenitor cells. Blood 121, 2891\u20132901. https:\/\/doi.org\/10.1182\/blood-2012-08-451864 (2013).","journal-title":"Blood"},{"key":"80050_CR66","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1111\/jcmm.12168","volume":"18","author":"N Espagnolle","year":"2014","unstructured":"Espagnolle, N. et al. CD146 expression on mesenchymal stem cells is associated with their vascular smooth muscle commitment. J. Cell. Mol. Med. 18, 104\u2013114 (2014).","journal-title":"J. Cell. Mol. Med."},{"key":"80050_CR67","doi-asserted-by":"publisher","first-page":"1564","DOI":"10.1074\/jbc.M007065200","volume":"276","author":"F Anfosso","year":"2001","unstructured":"Anfosso, F. et al. Outside-in signaling pathway linked to CD146 engagement in human endothelial cells. J. Biol. Chem. 276, 1564\u20131569. https:\/\/doi.org\/10.1074\/jbc.M007065200 (2001).","journal-title":"J. Biol. Chem."},{"key":"80050_CR68","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1016\/j.exphem.2007.12.015","volume":"36","author":"DT Covas","year":"2008","unstructured":"Covas, D. T. et al. Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts. Exp. Hematol. 36, 642\u2013654. https:\/\/doi.org\/10.1016\/j.exphem.2007.12.015 (2008).","journal-title":"Exp. Hematol."},{"key":"80050_CR69","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1016\/j.bbrc.2006.06.110","volume":"347","author":"AK Kundu","year":"2006","unstructured":"Kundu, A. K. & Putnam, A. J. Vitronectin and collagen I differentially regulate osteogenesis in mesenchymal stem cells. Biochem. Biophys. Res. Commun. 347, 347\u2013357. https:\/\/doi.org\/10.1016\/j.bbrc.2006.06.110 (2006).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"80050_CR70","doi-asserted-by":"publisher","first-page":"1094","DOI":"10.1016\/j.joca.2019.04.002","volume":"27","author":"S Wangler","year":"2019","unstructured":"Wangler, S. et al. CD146\/MCAM distinguishes stem cell subpopulations with distinct migration and regenerative potential in degenerative intervertebral discs. Osteoarthr. Cartil. 27, 1094\u20131105. https:\/\/doi.org\/10.1016\/j.joca.2019.04.002 (2019).","journal-title":"Osteoarthr. Cartil."},{"key":"80050_CR71","doi-asserted-by":"publisher","first-page":"1558","DOI":"10.1089\/scd.2014.0465","volume":"24","author":"C Ulrich","year":"2015","unstructured":"Ulrich, C. et al. Human placenta-derived CD146-positive mesenchymal stromal cells display a distinct osteogenic differentiation potential. Stem Cells Dev. 24, 1558\u20131569. https:\/\/doi.org\/10.1089\/scd.2014.0465 (2015).","journal-title":"Stem Cells Dev."},{"key":"80050_CR72","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.brainresbull.2021.09.008","volume":"177","author":"WP Xiong","year":"2021","unstructured":"Xiong, W. P., Yao, W. Q., Wang, B. & Liu, K. BMSCs-exosomes containing GDF-15 alleviated SH-SY5Y cell injury model of Alzheimer\u2019s disease via AKT\/GSK-3beta\/beta-catenin. Brain Res. Bull. 177, 92\u2013102. https:\/\/doi.org\/10.1016\/j.brainresbull.2021.09.008 (2021).","journal-title":"Brain Res. Bull."},{"key":"80050_CR73","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1016\/j.bone.2006.09.022","volume":"40","author":"Q Zeng","year":"2007","unstructured":"Zeng, Q., Li, X., Beck, G., Balian, G. & Shen, F. H. Growth and differentiation factor-5 (GDF-5) stimulates osteogenic differentiation and increases vascular endothelial growth factor (VEGF) levels in fat-derived stromal cells in vitro. Bone 40, 374\u2013381. https:\/\/doi.org\/10.1016\/j.bone.2006.09.022 (2007).","journal-title":"Bone"},{"key":"80050_CR74","doi-asserted-by":"publisher","first-page":"52294","DOI":"10.18632\/oncotarget.10561","volume":"7","author":"F Zhang","year":"2016","unstructured":"Zhang, F. et al. TGF-\u03b2 induces M2-like macrophage polarization via SNAIL-mediated suppression of a pro-inflammatory phenotype. Oncotarget 7, 52294\u201352306. https:\/\/doi.org\/10.18632\/oncotarget.10561 (2016).","journal-title":"Oncotarget"},{"key":"80050_CR75","doi-asserted-by":"publisher","first-page":"e0191040","DOI":"10.1371\/journal.pone.0191040","volume":"13","author":"KC Wheeler","year":"2018","unstructured":"Wheeler, K. C. et al. VEGF may contribute to macrophage recruitment and M2 polarization in the decidua. PLoS One 13, e0191040. https:\/\/doi.org\/10.1371\/journal.pone.0191040 (2018).","journal-title":"PLoS One"},{"key":"80050_CR76","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.imbio.2005.05.002","volume":"210","author":"KH Park-Min","year":"2005","unstructured":"Park-Min, K. H., Antoniv, T. T. & Ivashkiv, L. B. Regulation of macrophage phenotype by long-term exposure to IL-10. Immunobiology 210, 77\u201386. https:\/\/doi.org\/10.1016\/j.imbio.2005.05.002 (2005).","journal-title":"Immunobiology"},{"key":"80050_CR77","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1002\/jcb.30309","volume":"123","author":"S Saberianpour","year":"2022","unstructured":"Saberianpour, S. et al. Therapeutic effects of statins on osteoarthritis: A review. J. Cell. Biochem. 123, 1285\u20131297. https:\/\/doi.org\/10.1002\/jcb.30309 (2022).","journal-title":"J. Cell. Biochem."},{"key":"80050_CR78","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.3390\/pathogens10101287","volume":"10","author":"M Becerra-D\u00edaz","year":"2021","unstructured":"Becerra-D\u00edaz, M. et al. STAT1-dependent recruitment of Ly6C(hi)CCR2(+) inflammatory monocytes and M2 macrophages in a helminth infection. Pathogens 10, 1287. https:\/\/doi.org\/10.3390\/pathogens10101287 (2021).","journal-title":"Pathogens"},{"key":"80050_CR79","doi-asserted-by":"publisher","first-page":"2139","DOI":"10.3389\/fpsyg.2020.02139","volume":"11","author":"M Jankowski","year":"2020","unstructured":"Jankowski, M., Broderick, T. L. & Gutkowska, J. The role of oxytocin in cardiovascular protection. Front Psychol 11, 2139. https:\/\/doi.org\/10.3389\/fpsyg.2020.02139 (2020).","journal-title":"Front Psychol"},{"key":"80050_CR80","first-page":"169","volume":"4","author":"MA Ahmed","year":"2011","unstructured":"Ahmed, M. A. & Elosaily, G. M. Role of oxytocin in deceleration of early atherosclerotic inflammatory processes in adult male rats. Int. J. Clin. Exp. Med. 4, 169\u2013178 (2011).","journal-title":"Int. J. Clin. Exp. Med."},{"key":"80050_CR81","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1097\/PSY.0b013e3181d74c48","volume":"72","author":"DA Nation","year":"2010","unstructured":"Nation, D. A. et al. Oxytocin attenuates atherosclerosis and adipose tissue inflammation in socially isolated ApoE-\/- mice. Psychosom. Med. 72, 376\u2013382. https:\/\/doi.org\/10.1097\/PSY.0b013e3181d74c48 (2010).","journal-title":"Psychosom. Med."},{"key":"80050_CR82","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1016\/j.diabet.2017.10.004","volume":"44","author":"S Garrido-Urbani","year":"2018","unstructured":"Garrido-Urbani, S. et al. Inhibitory role of oxytocin on TNF\u03b1 expression assessed in vitro and in vivo. Diabetes Metab. 44, 292\u2013295. https:\/\/doi.org\/10.1016\/j.diabet.2017.10.004 (2018).","journal-title":"Diabetes Metab."},{"key":"80050_CR83","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1186\/s12944-020-01364-x","volume":"19","author":"A Szeto","year":"2020","unstructured":"Szeto, A., Cecati, M., Ahmed, R., McCabe, P. M. & Mendez, A. J. Oxytocin reduces adipose tissue inflammation in obese mice. Lipids Health Dis. 19, 188. https:\/\/doi.org\/10.1186\/s12944-020-01364-x (2020).","journal-title":"Lipids Health Dis."},{"key":"80050_CR84","doi-asserted-by":"publisher","first-page":"1977","DOI":"10.1042\/cs20190756","volume":"133","author":"Y Tang","year":"2019","unstructured":"Tang, Y. et al. Oxytocin system alleviates intestinal inflammation by regulating macrophages polarization in experimental colitis. Clin. Sci. 133, 1977\u20131992. https:\/\/doi.org\/10.1042\/cs20190756 (2019).","journal-title":"Clin. Sci."},{"key":"80050_CR85","doi-asserted-by":"publisher","first-page":"114867","DOI":"10.1016\/j.bcp.2021.114867","volume":"195","author":"Y Yu","year":"2022","unstructured":"Yu, Y., Li, J. & Liu, C. Oxytocin suppresses epithelial cell-derived cytokines production and alleviates intestinal inflammation in food allergy. Biochem. Pharmacol. 195, 114867. https:\/\/doi.org\/10.1016\/j.bcp.2021.114867 (2022).","journal-title":"Biochem. Pharmacol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-80050-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-80050-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-80050-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T07:06:38Z","timestamp":1732691198000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-80050-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,27]]},"references-count":85,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["80050"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-80050-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,27]]},"assertion":[{"value":"21 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"29438"}}