{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T05:28:57Z","timestamp":1778304537506,"version":"3.51.4"},"reference-count":117,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"Yunnan Province Basic Research Project","award":["202301AS070005"],"award-info":[{"award-number":["202301AS070005"]}]},{"name":"Yunnan Province Basic Research Project","award":["202101AT070060"],"award-info":[{"award-number":["202101AT070060"]}]},{"name":"the Earmarked Fund for China Agriculture Research System","award":["CARS-39"],"award-info":[{"award-number":["CARS-39"]}]},{"name":"Yunnan Young and Middle-Aged Academic and Technical Leaders Reserve Talent Program","award":["202305AC160033"],"award-info":[{"award-number":["202305AC160033"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-80556-2","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T12:47:14Z","timestamp":1732711634000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Integrating proteomics and metabolomics to evaluate impact of semen collection techniques on the quality and cryotolerance of goat semen"],"prefix":"10.1038","volume":"14","author":[{"given":"Chunyan","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiachong","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larbi","family":"Allai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bouabid","family":"Badaoui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyong","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yina","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoquan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guobo","family":"Quan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunrong","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,11,27]]},"reference":[{"key":"80556_CR1","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1089\/bio.2019.0128","volume":"18","author":"F Zomerdijk","year":"2020","unstructured":"Zomerdijk, F., Hiemstra, S. J., D\u2019Arbaumont, M., Tixier-Boichard, M. & Boettcher, P. Quality management practices of gene banks for livestock: a global review. Biopreserv. Biobank. 18, 244\u2013253 (2020).","journal-title":"Biopreserv. Biobank."},{"key":"80556_CR2","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.anireprosci.2016.02.013","volume":"167","author":"P Jimenez-Rabadan","year":"2016","unstructured":"Jimenez-Rabadan, P. et al. Influence of semen collection method on sperm cryoresistance in small ruminants. Anim. Reprod. Sci. 167, 103\u2013108 (2016).","journal-title":"Anim. Reprod. Sci."},{"key":"80556_CR3","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1038\/164666a0","volume":"164","author":"C Polge","year":"1949","unstructured":"Polge, C., Smith, A. U. & Parkes, A. S. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature. 164, 666 (1949).","journal-title":"Nature."},{"key":"80556_CR4","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1186\/s13148-022-01355-y","volume":"14","author":"H Chen","year":"2022","unstructured":"Chen, H., Zhang, L., Meng, L., Liang, L. & Zhang, C. Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes. Clin. Epigenetics. 14, 141 (2022).","journal-title":"Clin. Epigenetics."},{"key":"80556_CR5","doi-asserted-by":"publisher","first-page":"266","DOI":"10.3389\/fvets.2018.00266","volume":"5","author":"SR Sathe","year":"2018","unstructured":"Sathe, S. R. Laparoscopic artificial insemination technique in small ruminants-a procedure review. Front. Vet. Sci. 5, 266 (2018).","journal-title":"Front. Vet. Sci."},{"key":"80556_CR6","doi-asserted-by":"crossref","unstructured":"Evans, H. C. et al. Harnessing the value of reproductive hormones in cattle production with considerations to animal welfare and human health. J. Anim. Sci. 100, (2022).","DOI":"10.1093\/jas\/skac177"},{"key":"80556_CR7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2022.104791","volume":"273","author":"C Li","year":"2023","unstructured":"Li, C. et al. Changes on proteomic and metabolomic profiling of cryopreserved sperm effected by melatonin. J. Proteomics. 273, 104791 (2023).","journal-title":"J. Proteomics."},{"key":"80556_CR8","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1038\/s41684-022-01053-5","volume":"51","author":"T Takeo","year":"2022","unstructured":"Takeo, T. et al. Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat. Lab Anim. 51, 256\u2013274 (2022).","journal-title":"Lab Anim."},{"key":"80556_CR9","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2021.106719","volume":"226","author":"R Ungerfeld","year":"2021","unstructured":"Ungerfeld, R. et al. Seasonality of the stress response in goat bucks when there is use of electroejaculation for semen collection. Anim. Reprod. Sci. 226, 106719 (2021).","journal-title":"Anim. Reprod. Sci."},{"key":"80556_CR10","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.862100","volume":"9","author":"J Meuffels","year":"2022","unstructured":"Meuffels, J. et al. Arterial blood gases and cardiorespiratory parameters in etorphine-medetomidine-midazolam immobilized free-ranging and game-farmed southern white rhinoceroses (ceratotherium simum simum) undergoing electro-ejaculation. Front. Vet. Sci. 9, 862100 (2022).","journal-title":"Front. Vet. Sci."},{"key":"80556_CR11","doi-asserted-by":"crossref","unstructured":"Hermo, L., Pelletier, R. M., Cyr, D. G. & Smith, C. E. Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. part 1: background to spermatogenesis, spermatogonia, and spermatocytes. Microsc. Res. Tech. 73, 241\u2013278 (2010).","DOI":"10.1002\/jemt.20783"},{"key":"80556_CR12","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1093\/humupd\/dmy017","volume":"24","author":"J Castillo","year":"2018","unstructured":"Castillo, J., Jodar, M. & Oliva, R. The Contribution of human sperm proteins to the development and epigenome of the preimplantation embryo. Hum. Reprod. Update. 24, 535\u2013555 (2018).","journal-title":"Hum. Reprod. Update."},{"key":"80556_CR13","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.cryobiol.2013.08.002","volume":"67","author":"P Jimenez-Rabadan","year":"2013","unstructured":"Jimenez-Rabadan, P. et al. Improved cryopreservation protocol for blanca-celtiberica buck semen collected by electroejaculation. Cryobiology. 67, 251\u2013257 (2013).","journal-title":"Cryobiology."},{"key":"80556_CR14","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.theriogenology.2006.01.054","volume":"66","author":"D Zambelli","year":"2006","unstructured":"Zambelli, D. & Cunto, M. Semen collection in cats: techniques and analysis. Theriogenology. 66, 159\u2013165 (2006).","journal-title":"Theriogenology."},{"key":"80556_CR15","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.theriogenology.2021.05.004","volume":"171","author":"B Jia","year":"2021","unstructured":"Jia, B. et al. Trehalose modifies the protein profile of ram spermatozoa during cryopreservation. Theriogenology. 171, 21\u201329 (2021).","journal-title":"Theriogenology."},{"key":"80556_CR16","doi-asserted-by":"publisher","first-page":"7880","DOI":"10.18632\/aging.102295","volume":"11","author":"T Liu","year":"2019","unstructured":"Liu, T. et al. Mechanisms of ros-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa. Aging (Albany Ny). 11, 7880\u20137898 (2019).","journal-title":"Aging (Albany Ny)."},{"key":"80556_CR17","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1111\/j.1600-079X.2010.00843.x","volume":"50","author":"S Succu","year":"2011","unstructured":"Succu, S. et al. Melatonin protects ram spermatozoa from cryopreservation injuries in a dose-dependent manner. J. Pineal Res. 50, 310\u2013318 (2011).","journal-title":"J. Pineal Res."},{"key":"80556_CR18","doi-asserted-by":"publisher","first-page":"1004683","DOI":"10.3389\/fvets.2022.1004683","volume":"9","author":"B Xu","year":"2022","unstructured":"Xu, B. et al. Evaluation of lipidomic change in goat sperm after cryopreservation. Front. Vet. Sci. 9, 1004683 (2022).","journal-title":"Front. Vet. Sci."},{"key":"80556_CR19","doi-asserted-by":"crossref","unstructured":"Ruiz-Diaz, S. et al. Changes in the cellular distribution of tyrosine phosphorylation and its relationship with the acrosomal exocytosis and plasma membrane integrity during in vitro capacitation of frozen\/thawed bull spermatozoa. Int. J. Mol. Sci. 21, (2020).","DOI":"10.3390\/ijms21082725"},{"key":"80556_CR20","doi-asserted-by":"crossref","unstructured":"Vozaf, J. et al. The cryopreserved sperm traits of various ram breeds: towards biodiversity conservation. Animals. 12, (2022).","DOI":"10.3390\/ani12101311"},{"key":"80556_CR21","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.theriogenology.2013.09.004","volume":"81","author":"RP Amann","year":"2014","unstructured":"Amann, R. P. & Waberski, D. Computer-assisted sperm analysis (casa): capabilities and potential developments. Theriogenology. 81, 5\u201317 (2014).","journal-title":"Theriogenology."},{"key":"80556_CR22","doi-asserted-by":"crossref","unstructured":"Sun, L. et al. Does antioxidant mitoquinone (mitoq) ameliorate oxidative stress in frozen-thawed rooster sperm? Animals. 12, (2022).","DOI":"10.3390\/ani12223181"},{"key":"80556_CR23","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.theriogenology.2022.03.015","volume":"184","author":"B Jia","year":"2022","unstructured":"Jia, B. et al. Identification and validation of ram sperm proteins associated with cryoinjuries caused by the cryopreservation process. Theriogenology. 184, 191\u2013203 (2022).","journal-title":"Theriogenology."},{"key":"80556_CR24","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1007\/s00441-017-2743-4","volume":"372","author":"A Najafi","year":"2018","unstructured":"Najafi, A. et al. Melatonin affects membrane integrity, intracellular reactive oxygen species, caspase3 activity and akt phosphorylation in frozen thawed human sperm. Cell Tissue Res. 372, 149\u2013159 (2018).","journal-title":"Cell Tissue Res."},{"key":"80556_CR25","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118631","volume":"264","author":"H Wang","year":"2021","unstructured":"Wang, H. et al. TMT-based proteomics analysis to screen potential biomarkers of acute-phase tbi in rats. Life Sci. 264, 118631 (2021).","journal-title":"Life Sci."},{"key":"80556_CR26","doi-asserted-by":"publisher","first-page":"15562","DOI":"10.1038\/s41598-021-95138-9","volume":"11","author":"B Jia","year":"2021","unstructured":"Jia, B. et al. The Characteristics of proteome and metabolome associated with contrasting sperm motility in goat seminal plasma. Sci Rep. 11, 15562 (2021).","journal-title":"Sci Rep."},{"key":"80556_CR27","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1007\/978-1-0716-2978-9_6","volume":"2628","author":"Y Zhou","year":"2023","unstructured":"Zhou, Y. et al. High-throughput and in-depth proteomic profiling of 5 mul plasma and serum using tmtpro 16-plex. Methods Mol Biol. 2628, 81\u201392 (2023).","journal-title":"Methods Mol Biol."},{"key":"80556_CR28","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.110271","volume":"38","author":"X Bi","year":"2022","unstructured":"Bi, X. et al. Proteomic and metabolomic profiling of urine uncovers immune responses in patients with covid-19. Cell Reports. 38, 110271 (2022).","journal-title":"Cell Reports."},{"key":"80556_CR29","doi-asserted-by":"publisher","first-page":"1121","DOI":"10.1021\/acs.jproteome.6b00374","volume":"16","author":"FK Huang","year":"2017","unstructured":"Huang, F. K. et al. Deep coverage of global protein expression and phosphorylation in breast tumor cell lines using tmt 10-plex isobaric labeling. J. Proteome Res. 16, 1121\u20131132 (2017).","journal-title":"J. Proteome Res."},{"key":"80556_CR30","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2022.101043","volume":"45","author":"J Xie","year":"2023","unstructured":"Xie, J. et al. TMT-based proteomics analysis of growth advantage of triploid apostichopus japonicus. Comp. Biochem. Physiol. D-Genomics Proteomics. 45, 101043 (2023).","journal-title":"Comp. Biochem. Physiol. D-Genomics Proteomics."},{"key":"80556_CR31","doi-asserted-by":"publisher","first-page":"2185","DOI":"10.1039\/D0RA06677J","volume":"11","author":"Y Lu","year":"2021","unstructured":"Lu, Y. et al. Quantitative proteomics approach to investigate the antibacterial response of helicobacter pylori to daphnetin, a traditional chinese medicine monomer. Rsc Adv. 11, 2185\u20132193 (2021).","journal-title":"Rsc Adv."},{"key":"80556_CR32","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1111\/ejn.15904","volume":"57","author":"I Unal","year":"2023","unstructured":"Unal, I. et al. Identification of molecular network of gut-brain axis associated with neuroprotective effects of ppardelta-ligand erucic acid in rotenone-induced parkinson\u2019s disease model in zebrafish. Eur. J. Neurosci. 57, 585\u2013606 (2023).","journal-title":"Eur. J. Neurosci."},{"key":"80556_CR33","doi-asserted-by":"crossref","unstructured":"Orsburn, B. C. Proteome discoverer-a community enhanced data processing suite for protein informatics. Proteomes. 9, (2021).","DOI":"10.3390\/proteomes9010015"},{"key":"80556_CR34","doi-asserted-by":"publisher","first-page":"D353","DOI":"10.1093\/nar\/gkw1092","volume":"45","author":"M Kanehisa","year":"2017","unstructured":"Kanehisa, M., Furumichi, M., Tanabe, M., Sato, Y. & Morishima, K. KEGG: new perspectives on genomes, pathways, diseases and drugs. Nucleic Acids Res. 45, D353\u2013D361 (2017).","journal-title":"Nucleic Acids Res."},{"key":"80556_CR35","doi-asserted-by":"publisher","first-page":"7310","DOI":"10.1039\/D1FO01135A","volume":"12","author":"K Guan","year":"2021","unstructured":"Guan, K. et al. TMT-based quantitative proteomics analysis reveals the effect of bovine derived mfg-e8 against oxidative stress on rat l6 cells. Food Funct. 12, 7310\u20137320 (2021).","journal-title":"Food Funct."},{"key":"80556_CR36","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1021\/acs.jproteome.2c00049","volume":"21","author":"Y Zhang","year":"2022","unstructured":"Zhang, Y. et al. Global quantification of glutathione s-transferases in human serum using lc-ms\/ms coupled with affinity enrichment. J. Proteome Res. 21, 1311\u20131320 (2022).","journal-title":"J. Proteome Res."},{"key":"80556_CR37","doi-asserted-by":"crossref","unstructured":"Ai, J., Wu, Q., Battino, M., Bai, W. & Tian, L. Using untargeted metabolomics to profile the changes in roselle (hibiscus sabdariffa l.) anthocyanins during wine fermentation. Food Chem. 364, 130425 (2021).","DOI":"10.1016\/j.foodchem.2021.130425"},{"key":"80556_CR38","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2022.981173","volume":"10","author":"X Zhang","year":"2022","unstructured":"Zhang, X. et al. Identification and mechanism prediction of mulberroside a metabolites in vivo and in vitro of rats using an integrated strategy of uhplc-q-exactive plus orbitrap ms and network pharmacology. Front. Chem. 10, 981173 (2022).","journal-title":"Front. Chem."},{"key":"80556_CR39","doi-asserted-by":"publisher","first-page":"896","DOI":"10.3389\/fendo.2019.00896","volume":"10","author":"Y Fang","year":"2019","unstructured":"Fang, Y., Zhao, C., Xiang, H., Zhao, X. & Zhong, R. Melatonin inhibits formation of mitochondrial permeability transition pores and improves oxidative phosphorylation of frozen-thawed ram sperm. Front. Endocrinol. 10, 896 (2019).","journal-title":"Front. Endocrinol."},{"key":"80556_CR40","doi-asserted-by":"publisher","first-page":"W216","DOI":"10.1093\/nar\/gkac194","volume":"50","author":"BT Sherman","year":"2022","unstructured":"Sherman, B. T. et al. David: A web server for functional enrichment analysis and functional annotation of gene lists (2021 update). Nucleic Acids Res. 50, W216\u2013W221 (2022).","journal-title":"Nucleic Acids Res."},{"key":"80556_CR41","doi-asserted-by":"publisher","first-page":"D458","DOI":"10.1093\/nar\/gkaa937","volume":"49","author":"I Letunic","year":"2021","unstructured":"Letunic, I., Khedkar, S. & Bork, P. Smart: recent updates, new developments and status in 2020. Nucleic Acids Res. 49, D458\u2013D460 (2021).","journal-title":"Nucleic Acids Res."},{"key":"80556_CR42","doi-asserted-by":"crossref","unstructured":"Wishart, D. S. et al. Hmdb 5.0: The human metabolome database for 2022. Nucleic Acids Res. 50, D622-D631 (2022).","DOI":"10.1093\/nar\/gkab1062"},{"key":"80556_CR43","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1095\/biolreprod.115.128553","volume":"94","author":"VM Salmon","year":"2016","unstructured":"Salmon, V. M., Leclerc, P. & Bailey, J. L. Cholesterol-loaded cyclodextrin increases the cholesterol content of goat sperm to improve cold and osmotic resistance and maintain sperm function after cryopreservation. Biol. Reprod. 94, 85 (2016).","journal-title":"Biol. Reprod."},{"key":"80556_CR44","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1093\/biolre\/iox096","volume":"97","author":"MS Lehti","year":"2017","unstructured":"Lehti, M. S. & Sironen, A. Formation and function of sperm tail structures in association with sperm motility defects. Biol. Reprod. 97, 522\u2013536 (2017).","journal-title":"Biol. Reprod."},{"key":"80556_CR45","doi-asserted-by":"publisher","first-page":"1827","DOI":"10.1093\/humrep\/14.7.1827","volume":"14","author":"PS James","year":"1999","unstructured":"James, P. S. et al. Lipid dynamics in the plasma membrane of fresh and cryopreserved human spermatozoa. Hum. Reprod. 14, 1827\u20131832 (1999).","journal-title":"Hum. Reprod."},{"key":"80556_CR46","doi-asserted-by":"publisher","first-page":"578","DOI":"10.4103\/aja.aja_2_20","volume":"22","author":"I Palacin","year":"2020","unstructured":"Palacin, I. et al. Relationship of sperm plasma membrane and acrosomal integrities with sperm morphometry in bos taurus. Asian J. Androl. 22, 578\u2013582 (2020).","journal-title":"Asian J. Androl."},{"key":"80556_CR47","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.rbmo.2021.05.005","volume":"43","author":"M Hezavehei","year":"2021","unstructured":"Hezavehei, M., Sharafi, M., Fathi, R., Shahverdi, A. & Gilani, M. Membrane lipid replacement with nano-micelles in human sperm cryopreservation improves post-thaw function and acrosome protein integrity. Reprod. Biomed. Online. 43, 257\u2013268 (2021).","journal-title":"Reprod. Biomed. Online."},{"key":"80556_CR48","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.2164\/jandrol.112.016535","volume":"33","author":"RJ Aitken","year":"2012","unstructured":"Aitken, R. J., Jones, K. T. & Robertson, S. A. Reactive oxygen species and sperm function\u2013in sickness and in health. J Androl. 33, 1096\u20131106 (2012).","journal-title":"J Androl."},{"key":"80556_CR49","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.theriogenology.2021.01.007","volume":"164","author":"HT Guo","year":"2021","unstructured":"Guo, H. T. et al. Effects of astaxanthin on plasma membrane function and fertility of boar sperm during cryopreservation. Theriogenology. 164, 58\u201364 (2021).","journal-title":"Theriogenology."},{"key":"80556_CR50","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1007\/s00018-019-03253-8","volume":"77","author":"E Barati","year":"2020","unstructured":"Barati, E., Nikzad, H. & Karimian, M. Oxidative stress and male infertility: current knowledge of pathophysiology and role of antioxidant therapy in disease management. Cell. Mol. Life Sci. 77, 93\u2013113 (2020).","journal-title":"Cell. Mol. Life Sci."},{"key":"80556_CR51","doi-asserted-by":"crossref","unstructured":"Hermo, L., Pelletier, R. M., Cyr, D. G. & Smith, C. E. Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. part 2: changes in spermatid organelles associated with development of spermatozoa. Microsc. Res. Tech. 73, 279\u2013319 (2010).","DOI":"10.1002\/jemt.20787"},{"key":"80556_CR52","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1095\/biolreprod.112.104208","volume":"87","author":"T Chauvin","year":"2012","unstructured":"Chauvin, T. et al. A systematic analysis of a deep mouse epididymal sperm proteome. Biol. Reprod. 87, 141 (2012).","journal-title":"Biol. Reprod."},{"key":"80556_CR53","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1017\/S0031182020002425","volume":"148","author":"P Cermakova","year":"2021","unstructured":"Cermakova, P. et al. Differences in mitochondrial nadh dehydrogenase activities in trypanosomatids. Parasitology. 148, 1161\u20131170 (2021).","journal-title":"Parasitology."},{"key":"80556_CR54","doi-asserted-by":"publisher","first-page":"2623","DOI":"10.1128\/MCB.01476-12","volume":"33","author":"B Pereira","year":"2013","unstructured":"Pereira, B., Videira, A. & Duarte, M. Novel insights into the role of neurospora crassa ndufaf2, an evolutionarily conserved mitochondrial complex i assembly factor. Mol. Cell. Biol. 33, 2623\u20132634 (2013).","journal-title":"Mol. Cell. Biol."},{"key":"80556_CR55","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1515\/hsz-2022-0309","volume":"404","author":"T Arroum","year":"2023","unstructured":"Arroum, T. et al. Loss of respiratory complex i subunit ndufb10 affects complex i assembly and supercomplex formation. Biol. Chem. 404, 399\u2013415 (2023).","journal-title":"Biol. Chem."},{"key":"80556_CR56","doi-asserted-by":"publisher","first-page":"4652","DOI":"10.1038\/emboj.2011.324","volume":"30","author":"T Althoff","year":"2011","unstructured":"Althoff, T., Mills, D. J., Popot, J. L. & Kuhlbrandt, W. Arrangement of electron transport chain components in bovine mitochondrial supercomplex i1iii2iv1. Embo J. 30, 4652\u20134664 (2011).","journal-title":"Embo J."},{"key":"80556_CR57","doi-asserted-by":"publisher","first-page":"2232","DOI":"10.1038\/s41467-022-29893-2","volume":"13","author":"H Guo","year":"2022","unstructured":"Guo, H. & Rubinstein, J. L. Structure of atp synthase under strain during catalysis. Nat. Commun. 13, 2232 (2022).","journal-title":"Nat. Commun."},{"key":"80556_CR58","doi-asserted-by":"publisher","first-page":"4247","DOI":"10.1111\/bph.14513","volume":"176","author":"V Giorgio","year":"2019","unstructured":"Giorgio, V., Fogolari, F., Lippe, G. & Bernardi, P. Oscp subunit of mitochondrial atp synthase: role in regulation of enzyme function and of its transition to a pore. Br. J. Pharmacol. 176, 4247\u20134257 (2019).","journal-title":"Br. J. Pharmacol."},{"key":"80556_CR59","doi-asserted-by":"publisher","first-page":"1162","DOI":"10.1016\/j.bbabio.2006.04.007","volume":"1757","author":"J Weber","year":"2006","unstructured":"Weber, J. Atp synthase: subunit-subunit interactions in the stator stalk. Biochim Biophys Acta. 1757, 1162\u20131170 (2006).","journal-title":"Biochim Biophys Acta."},{"key":"80556_CR60","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1016\/j.ajhg.2008.03.020","volume":"82","author":"O Barel","year":"2008","unstructured":"Barel, O. et al. Mitochondrial complex iii deficiency associated with a homozygous mutation in uqcrq. Am. J. Hum. Genet. 82, 1211\u20131216 (2008).","journal-title":"Am. J. Hum. Genet."},{"key":"80556_CR61","doi-asserted-by":"publisher","first-page":"1346","DOI":"10.1016\/j.bbabio.2012.11.015","volume":"1827","author":"B Meunier","year":"2013","unstructured":"Meunier, B., Fisher, N., Ransac, S., Mazat, J. P. & Brasseur, G. Respiratory complex iii dysfunction in humans and the use of yeast as a model organism to study mitochondrial myopathy and associated diseases. Biochim Biophys Acta. 1827, 1346\u20131361 (2013).","journal-title":"Biochim Biophys Acta."},{"key":"80556_CR62","doi-asserted-by":"crossref","unstructured":"Cheng, C. C. et al. Sperm-specific cox6b2 enhances oxidative phosphorylation, proliferation, and survival in human lung adenocarcinoma. Elife. 9, (2020).","DOI":"10.7554\/eLife.58108"},{"key":"80556_CR63","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1097\/01.fpc.0000165725.81559.e3","volume":"15","author":"EM Schmuck","year":"2005","unstructured":"Schmuck, E. M. et al. Characterization of the monomethylarsonate reductase and dehydroascorbate reductase activities of omega class glutathione transferase variants: implications for arsenic metabolism and the age-at-onset of alzheimer\u2019s and parkinson\u2019s diseases. Pharmacogenet. Genomics. 15, 493\u2013501 (2005).","journal-title":"Pharmacogenet. Genomics."},{"key":"80556_CR64","doi-asserted-by":"crossref","unstructured":"Hamilton, L. E. et al. Gsto2 isoforms participate in the oxidative regulation of the plasmalemma in eutherian spermatozoa during capacitation. Antioxidants. 8, (2019).","DOI":"10.3390\/antiox8120601"},{"key":"80556_CR65","doi-asserted-by":"crossref","unstructured":"Llavanera, M. et al. Gstm3, but not izumo1, is a cryotolerance marker of boar sperm. J. Anim. Sci. Biotechnol. 10, (2019).","DOI":"10.1186\/s40104-019-0370-5"},{"key":"80556_CR66","doi-asserted-by":"crossref","unstructured":"Llavanera, M. et al. Exploring seminal plasma gstm3 as a quality and in vivo fertility biomarker in pigs-relationship with sperm morphology. Antioxidants. 9, (2020).","DOI":"10.3390\/antiox9080741"},{"key":"80556_CR67","doi-asserted-by":"publisher","first-page":"291","DOI":"10.15252\/embj.201695021","volume":"36","author":"S Shimada","year":"2017","unstructured":"Shimada, S. et al. Complex structure of cytochrome c-cytochrome c oxidase reveals a novel protein-protein interaction mode. Embo J. 36, 291\u2013300 (2017).","journal-title":"Embo J."},{"key":"80556_CR68","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111899","volume":"42","author":"A Bassot","year":"2023","unstructured":"Bassot, A. et al. The endoplasmic reticulum kinase perk interacts with the oxidoreductase ero1 to metabolically adapt mitochondria. Cell Reports. 42, 111899 (2023).","journal-title":"Cell Reports."},{"key":"80556_CR69","doi-asserted-by":"publisher","first-page":"44604","DOI":"10.1007\/s11356-020-11070-x","volume":"27","author":"IM Saadeldin","year":"2020","unstructured":"Saadeldin, I. M. et al. Vitrification of camel oocytes transiently impacts mitochondrial functions without affecting the developmental potential after intracytoplasmic sperm injection and parthenogenetic activation. Environ. Sci. Pollut. Res. 27, 44604\u201344613 (2020).","journal-title":"Environ. Sci. Pollut. Res."},{"key":"80556_CR70","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102387","volume":"298","author":"S Zhu","year":"2022","unstructured":"Zhu, S. et al. Isocitrate dehydrogenase 3b is required for spermiogenesis but dispensable for retinal viability. J. Biol. Chem. 298, 102387 (2022).","journal-title":"J. Biol. Chem."},{"key":"80556_CR71","doi-asserted-by":"publisher","first-page":"1166232","DOI":"10.3389\/fcell.2023.1166232","volume":"11","author":"M Horta Remedios","year":"2023","unstructured":"Horta Remedios, M. et al. Ether lipids and a peroxisomal riddle in sperm. Front. Cell. Dev. Biol. 11, 1166232 (2023).","journal-title":"Front. Cell. Dev. Biol."},{"key":"80556_CR72","doi-asserted-by":"crossref","unstructured":"Mostek, A., Janta, A., Majewska, A. & Ciereszko, A. Bull sperm capacitation is accompanied by redox modifications of proteins. Int. J. Mol. Sci. 22, (2021).","DOI":"10.3390\/ijms22157903"},{"key":"80556_CR73","doi-asserted-by":"crossref","unstructured":"Park, Y. J. & Pang, M. G. Mitochondrial functionality in male fertility: from spermatogenesis to fertilization. Antioxidants. 10, (2021).","DOI":"10.3390\/antiox10010098"},{"key":"80556_CR74","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125728","volume":"246","author":"W Qin","year":"2023","unstructured":"Qin, W. et al. Comparative itraq proteomics identified proteins in fresh and frozen thawed yak spermatozoa. Int. J. Biol. Macromol. 246, 125728 (2023).","journal-title":"Int. J. Biol. Macromol."},{"key":"80556_CR75","doi-asserted-by":"crossref","unstructured":"Morohoshi, A. et al. Fam71f1 binds to rab2a and rab2b and is essential for acrosome formation and male fertility in mice. Development. 148, (2021).","DOI":"10.1242\/dev.199644"},{"key":"80556_CR76","doi-asserted-by":"publisher","first-page":"2730","DOI":"10.1093\/brain\/awad068","volume":"146","author":"A Nasca","year":"2023","unstructured":"Nasca, A. et al. Variants in atp5f1b are associated with dominantly inherited dystonia. Brain. 146, 2730\u20132738 (2023).","journal-title":"Brain."},{"key":"80556_CR77","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.tox.2016.06.003","volume":"357\u2013358","author":"X Li","year":"2016","unstructured":"Li, X. et al. Tyrosine phosphorylation of dihydrolipoamide dehydrogenase as a potential cadmium target and its inhibitory role in regulating mouse sperm motility. Toxicology. 357\u2013358, 52\u201364 (2016).","journal-title":"Toxicology."},{"key":"80556_CR78","doi-asserted-by":"crossref","unstructured":"M, K. M., Kumaresan, A., Yadav, S., Mohanty, T. K. & Datta, T. K. Comparative proteomic analysis of high- and low-fertile buffalo bull spermatozoa for identification of fertility-associated proteins. Reprod. Domest. Anim. 54, 786\u2013794 (2019).","DOI":"10.1111\/rda.13426"},{"key":"80556_CR79","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.950979","volume":"10","author":"M Tourmente","year":"2022","unstructured":"Tourmente, M., Sansegundo, E., Rial, E. & Roldan, E. Capacitation promotes a shift in energy metabolism in murine sperm. Front. Cell. Dev. Biol. 10, 950979 (2022).","journal-title":"Front. Cell. Dev. Biol."},{"key":"80556_CR80","doi-asserted-by":"publisher","first-page":"9987","DOI":"10.1038\/s41598-019-46446-8","volume":"9","author":"G Barshad","year":"2019","unstructured":"Barshad, G., Zlotnikov-Poznianski, N., Gal, L., Schuldiner, M. & Mishmar, D. Disease-causing mutations in subunits of oxphos complex i affect certain physical interactions. Sci Rep. 9, 9987 (2019).","journal-title":"Sci Rep."},{"key":"80556_CR81","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1016\/j.bbabio.2011.08.010","volume":"1817","author":"M Mimaki","year":"2012","unstructured":"Mimaki, M., Wang, X., McKenzie, M., Thorburn, D. R. & Ryan, M. T. understanding mitochondrial complex i assembly in health and disease. Biochim Biophys Acta. 1817, 851\u2013862 (2012).","journal-title":"Biochim Biophys Acta."},{"key":"80556_CR82","doi-asserted-by":"publisher","first-page":"4541","DOI":"10.1093\/hmg\/ddx333","volume":"26","author":"S Raffa","year":"2017","unstructured":"Raffa, S. et al. In vitro characterization of mitochondrial function and structure in rat and human cells with a deficiency of the nadh: ubiquinone oxidoreductase ndufc2 subunit. Hum. Mol. Genet. 26, 4541\u20134555 (2017).","journal-title":"Hum. Mol. Genet."},{"key":"80556_CR83","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2020.148213","volume":"1861","author":"M Adjobo-Hermans","year":"2020","unstructured":"Adjobo-Hermans, M. et al. Ndufs4 deletion triggers loss of ndufa12 in ndufs4(-\/-) mice and leigh syndrome patients: a stabilizing role for ndufaf2. Biochim. Biophys. Acta-Bioenerg. 1861, 148213 (2020).","journal-title":"Biochim. Biophys. Acta-Bioenerg."},{"key":"80556_CR84","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.693683","volume":"12","author":"D Pesta","year":"2021","unstructured":"Pesta, D. et al. Ndufb6 polymorphism is associated with physical activity-mediated metabolic changes in type 2 diabetes. Front. Endocrinol. 12, 693683 (2021).","journal-title":"Front. Endocrinol."},{"key":"80556_CR85","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1002\/humu.24135","volume":"42","author":"G Helman","year":"2021","unstructured":"Helman, G. et al. Multiomic analysis elucidates complex i deficiency caused by a deep intronic variant in ndufb10. Hum. Mutat. 42, 19\u201324 (2021).","journal-title":"Hum. Mutat."},{"key":"80556_CR86","doi-asserted-by":"crossref","unstructured":"He, Q. et al. Alkaline dilution alters sperm motility in dairy goat by affecting sac\/camp\/pka pathway activity. Int. J. Mol. Sci. 24, (2023).","DOI":"10.3390\/ijms24021771"},{"key":"80556_CR87","first-page":"621","volume":"37","author":"KQ Chen","year":"2022","unstructured":"Chen, K. Q., Wei, B. H., Hao, S. L. & Yang, W. X. The pi3k\/akt signaling pathway: how does it regulate development of sertoli cells and spermatogenic cells?. Histol. Histopath. 37, 621\u2013636 (2022).","journal-title":"Histol. Histopath."},{"key":"80556_CR88","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/s13287-022-02748-9","volume":"13","author":"B Li","year":"2022","unstructured":"Li, B. et al. Attenuates of nad(+) impair bmsc osteogenesis and fracture repair through oxphos. Stem Cell Res. Ther. 13, 77 (2022).","journal-title":"Stem Cell Res. Ther."},{"key":"80556_CR89","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1016\/j.molcel.2020.12.012","volume":"81","author":"A Luengo","year":"2021","unstructured":"Luengo, A. et al. Increased demand for nad(+) relative to atp drives aerobic glycolysis. Mol. Cell. 81, 691\u2013707 (2021).","journal-title":"Mol. Cell."},{"key":"80556_CR90","doi-asserted-by":"publisher","first-page":"1136","DOI":"10.1124\/mol.111.073916","volume":"80","author":"M Pittelli","year":"2011","unstructured":"Pittelli, M. et al. Pharmacological effects of exogenous nad on mitochondrial bioenergetics, DNA repair, and apoptosis. Mol. Pharmacol. 80, 1136\u20131146 (2011).","journal-title":"Mol. Pharmacol."},{"key":"80556_CR91","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/s41421-020-0183-x","volume":"6","author":"W Chen","year":"2020","unstructured":"Chen, W. et al. A bioenergetic shift is required for spermatogonial differentiation. Cell Discov. 6, 56 (2020).","journal-title":"Cell Discov."},{"key":"80556_CR92","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1186\/s12894-022-01107-3","volume":"22","author":"X Bai","year":"2022","unstructured":"Bai, X. & Wang, P. Relationship between sperm nad + concentration and reproductive aging in normozoospermia men: a cohort study. Bmc Urol. 22, 159 (2022).","journal-title":"Bmc Urol."},{"key":"80556_CR93","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1007\/978-1-60761-756-3_18","volume":"648","author":"JB Owen","year":"2010","unstructured":"Owen, J. B. & Butterfield, D. A. Measurement of oxidized\/reduced glutathione ratio. Methods Mol Biol. 648, 269\u2013277 (2010).","journal-title":"Methods Mol Biol."},{"key":"80556_CR94","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1089\/rej.2006.9.169","volume":"9","author":"DP Jones","year":"2006","unstructured":"Jones, D. P. Extracellular redox state: refining the definition of oxidative stress in aging. Rejuv. Res. 9, 169\u2013181 (2006).","journal-title":"Rejuv. Res."},{"key":"80556_CR95","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.freeradbiomed.2020.07.017","volume":"160","author":"G Bjorklund","year":"2020","unstructured":"Bjorklund, G. et al. The role of glutathione redox imbalance in autism spectrum disorder: a review. Free Radic. Biol. Med. 160, 149\u2013162 (2020).","journal-title":"Free Radic. Biol. Med."},{"key":"80556_CR96","doi-asserted-by":"publisher","first-page":"2192093","DOI":"10.1155\/2019\/2192093","volume":"2019","author":"E Moretti","year":"2019","unstructured":"Moretti, E. et al. Resistin in human seminal plasma: relationship with lipid peroxidation, cat activity, GSH\/GSSG ratio, and semen parameters. Oxidative Med. Cell. Longev. 2019, 2192093 (2019).","journal-title":"Oxidative Med. Cell. Longev."},{"key":"80556_CR97","doi-asserted-by":"publisher","first-page":"5352","DOI":"10.1002\/ptr.7144","volume":"35","author":"A Hosseini","year":"2021","unstructured":"Hosseini, A., Razavi, B. M., Banach, M. & Hosseinzadeh, H. Quercetin and metabolic syndrome: a review. Phytother. Res. 35, 5352\u20135364 (2021).","journal-title":"Phytother. Res."},{"key":"80556_CR98","doi-asserted-by":"crossref","unstructured":"Xu, D., Hu, M. J., Wang, Y. Q. & Cui, Y. L. Antioxidant activities of quercetin and its complexes for medicinal application. Molecules. 24, (2019).","DOI":"10.3390\/molecules24061123"},{"key":"80556_CR99","doi-asserted-by":"publisher","first-page":"21925","DOI":"10.1007\/s11356-021-17421-6","volume":"29","author":"S Bang","year":"2022","unstructured":"Bang, S. et al. Quercetin improves the apoptotic index and oxidative stress in post-thaw dog sperm. Environ. Sci. Pollut. Res. 29, 21925\u201321934 (2022).","journal-title":"Environ. Sci. Pollut. Res."},{"key":"80556_CR100","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.604460","volume":"7","author":"A El-Khawagah","year":"2020","unstructured":"El-Khawagah, A., Kandiel, M. & Samir, H. Effect of quercetin supplementation in extender on sperm kinematics, extracellular enzymes release, and oxidative stress of egyptian buffalo bulls frozen-thawed semen. Front. Vet. Sci. 7, 604460 (2020).","journal-title":"Front. Vet. Sci."},{"key":"80556_CR101","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1016\/j.theriogenology.2012.06.032","volume":"79","author":"Z Gibb","year":"2013","unstructured":"Gibb, Z., Butler, T. J., Morris, L. H., Maxwell, W. M. & Grupen, C. G. Quercetin improves the postthaw characteristics of cryopreserved sex-sorted and nonsorted stallion sperm. Theriogenology. 79, 1001\u20131009 (2013).","journal-title":"Theriogenology."},{"key":"80556_CR102","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134420","volume":"10","author":"TM Nguyen","year":"2015","unstructured":"Nguyen, T. M., Seigneurin, F., Froment, P., Combarnous, Y. & Blesbois, E. The 5\u2019-amp-activated protein kinase (ampk) is involved in the augmentation of antioxidant defenses in cryopreserved chicken sperm. Plos One. 10, e0134420 (2015).","journal-title":"Plos One."},{"key":"80556_CR103","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1007\/s10561-017-9642-5","volume":"19","author":"NM Shabani","year":"2018","unstructured":"Shabani, N. M., Nekoonam, S., Naji, M., Bakhshalizadeh, S. & Amidi, F. Cryoprotective effect of resveratrol on dna damage and crucial human sperm messenger rnas, possibly through 5\u2019 AMP-activated protein kinase activation. Cell Tissue Banking. 19, 87\u201395 (2018).","journal-title":"Cell Tissue Banking."},{"key":"80556_CR104","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1007\/s10815-019-01470-5","volume":"36","author":"P Thuwanut","year":"2019","unstructured":"Thuwanut, P., Comizzoli, P., Pruksananonda, K., Chatdarong, K. & Songsasen, N. Activation of adenosine monophosphate-activated protein kinase (ampk) enhances energy metabolism, motility, and fertilizing ability of cryopreserved spermatozoa in domestic cat model. J. Assist. Reprod. Genet. 36, 1401\u20131412 (2019).","journal-title":"J. Assist. Reprod. Genet."},{"key":"80556_CR105","doi-asserted-by":"publisher","first-page":"18192","DOI":"10.1002\/jcp.28452","volume":"234","author":"K Feng","year":"2019","unstructured":"Feng, K., Chen, Z., Pengcheng, L., Zhang, S. & Wang, X. Quercetin attenuates oxidative stress-induced apoptosis via sirt1\/ampk-mediated inhibition of er stress in rat chondrocytes and prevents the progression of osteoarthritis in a rat model. J. Cell. Physiol. 234, 18192\u201318205 (2019).","journal-title":"J. Cell. Physiol."},{"key":"80556_CR106","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1104\/pp.18.01346","volume":"180","author":"L Rosado-Souza","year":"2019","unstructured":"Rosado-Souza, L. et al. Appropriate thiamin pyrophosphate levels are required for acclimation to changes in photoperiod. Plant Physiol. 180, 185\u2013197 (2019).","journal-title":"Plant Physiol."},{"key":"80556_CR107","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1104\/pp.104.058693","volume":"138","author":"IP Ahn","year":"2005","unstructured":"Ahn, I. P., Kim, S. & Lee, Y. H. Vitamin B1 functions as an activator of plant disease resistance. Plant Physiol. 138, 1505\u20131515 (2005).","journal-title":"Plant Physiol."},{"key":"80556_CR108","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/1471-2091-8-10","volume":"8","author":"P Fraccascia","year":"2007","unstructured":"Fraccascia, P., Sniekers, M., Casteels, M. & Van Veldhoven, P. P. Presence of thiamine pyrophosphate in mammalian peroxisomes. Bmc Boichem. 8, 10 (2007).","journal-title":"Bmc Boichem."},{"key":"80556_CR109","doi-asserted-by":"publisher","first-page":"1148166","DOI":"10.3389\/fendo.2023.1148166","volume":"14","author":"U Kansakar","year":"2023","unstructured":"Kansakar, U. et al. Choline supplements: an update. Front. Endocrinol. 14, 1148166 (2023).","journal-title":"Front. Endocrinol."},{"key":"80556_CR110","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.taap.2016.09.018","volume":"310","author":"J Zhang","year":"2016","unstructured":"Zhang, J. et al. Choline supplementation alleviates fluoride-induced testicular toxicity by restoring the NGF and MEK expression in mice. Toxicol. Appl. Pharmacol. 310, 205\u2013214 (2016).","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"80556_CR111","doi-asserted-by":"publisher","first-page":"3741","DOI":"10.1007\/s00394-022-02917-9","volume":"61","author":"MM Billah","year":"2022","unstructured":"Billah, M. M., Khatiwada, S., Lecomte, V., Morris, M. J. & Maloney, C. A. Ameliorating high-fat diet-induced sperm and testicular oxidative damage by micronutrient-based antioxidant intervention in rats. Eur. J. Nutr. 61, 3741\u20133753 (2022).","journal-title":"Eur. J. Nutr."},{"key":"80556_CR112","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1095\/biolreprod.104.035238","volume":"72","author":"TL Kelly","year":"2005","unstructured":"Kelly, T. L., Neaga, O. R., Schwahn, B. C., Rozen, R. & Trasler, J. M. Infertility in 5,10-methylenetetrahydrofolate reductase (MTHFR)-deficient male mice is partially alleviated by lifetime dietary betaine supplementation. Biol. Reprod. 72, 667\u2013677 (2005).","journal-title":"Biol. Reprod."},{"key":"80556_CR113","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/j.cryobiol.2022.03.006","volume":"106","author":"N Mori","year":"2022","unstructured":"Mori, N., Ishihara, M., Tasaki, H., Sankai, T. & Otsuki, J. The effect of betaine for mouse sperm cryopreservation. Cryobiology. 106, 157\u2013159 (2022).","journal-title":"Cryobiology."},{"key":"80556_CR114","doi-asserted-by":"publisher","first-page":"1588","DOI":"10.1016\/j.biortech.2017.05.145","volume":"245","author":"Y Li","year":"2017","unstructured":"Li, Y. et al. Current status on metabolic engineering for the production of l-aspartate family amino acids and derivatives. Bioresour. Technol. 245, 1588\u20131602 (2017).","journal-title":"Bioresour. Technol."},{"key":"80556_CR115","doi-asserted-by":"crossref","unstructured":"Han, M. et al. L-aspartate: an essential metabolite for plant growth and stress acclimation. Molecules. 26, (2021).","DOI":"10.3390\/molecules26071887"},{"key":"80556_CR116","doi-asserted-by":"publisher","first-page":"3282","DOI":"10.1080\/15384101.2015.1087619","volume":"14","author":"K Dornfeld","year":"2015","unstructured":"Dornfeld, K., Madden, M., Skildum, A. & Wallace, K. B. Aspartate facilitates mitochondrial function, growth arrest and survival during doxorubicin exposure. Cell Cycle. 14, 3282\u20133291 (2015).","journal-title":"Cell Cycle."},{"key":"80556_CR117","doi-asserted-by":"publisher","first-page":"1132373","DOI":"10.3389\/fvets.2023.1132373","volume":"10","author":"B Xu","year":"2023","unstructured":"Xu, B. et al. Metabolomic analysis of seminal plasma to identify goat semen freezability markers. Front. Vet. Sci. 10, 1132373 (2023).","journal-title":"Front. Vet. Sci."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-80556-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-80556-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-80556-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T14:08:54Z","timestamp":1732716534000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-80556-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,27]]},"references-count":117,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["80556"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-80556-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,27]]},"assertion":[{"value":"20 January 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"29489"}}