{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:17:40Z","timestamp":1789471060442,"version":"build-2803163510"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100013420","name":"Tsumura and Company","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013420","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Aquaporin 2 (AQP2) contributes to water reabsorption and urine concentration by migrating to the luminal surface of the collecting ducts in an anti-diuretic hormone-stimulated manner, and the signaling pathway involved in AQP2 subcellular localization is a target for arginine vasopressin receptor antagonists (aquaretics). This study investigated the involvement of AQP2 in the diuretic effect and mechanisms of Goreisan (GRS), a traditional Japanese Kampo medicine used to treat conditions such as edema in patients with decreased urination. GRS exerted diuretic effects on desmopressin (DDAVP)-induced decreases in urine output and the level of AQP2 phosphorylated at Serine269 (pSer269-AQP2) in the renal tissues of mice. Furthermore, GRS inhibited the accumulation of pSer269-AQP2 to the luminal side following forskolin stimulation using a 3D culture model of the kidney collecting duct cell line mIMCD-3. GRS induced a transient increase in the intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration via the calcium-sensing receptor (CaSR) and suppressed the forskolin-stimulated increase in cAMP production. These results suggest that GRS regulates urine volume by modulating the subcellular localization of AQP2 via CaSR.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-81324-y","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T14:31:20Z","timestamp":1732804280000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Goreisan promotes diuresis by regulating the abundance of aquaporin 2 phosphorylated at serine 269 through calcium-sensing receptor activation"],"prefix":"10.1038","volume":"14","author":[{"given":"Keisuke","family":"Ogura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Fujitsuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miwa","family":"Nahata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Tokita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,28]]},"reference":[{"key":"81324_CR1","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/S1357-4310(99)01636-6","volume":"6","author":"LS King","year":"2000","unstructured":"King, L. S., Yasui, M. & Agre, P. Aquaporins in health and disease. Mol. Med. Today. 6, 60\u201365. https:\/\/doi.org\/10.1016\/S1357-4310(99)01636-6 (2000).","journal-title":"Mol. Med. Today"},{"key":"81324_CR2","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1038\/nrd4226","volume":"13","author":"AS Verkman","year":"2014","unstructured":"Verkman, A. S., Anderson, M. O. & Papadopoulos, M. C. Aquaporins: Important but elusive drug targets. Nat. Rev. Drug Discov. 13, 259\u2013277. https:\/\/doi.org\/10.1038\/nrd4226 (2014).","journal-title":"Nat. Rev. Drug Discov."},{"key":"81324_CR3","doi-asserted-by":"publisher","first-page":"4296","DOI":"10.1074\/jbc.273.8.4296","volume":"273","author":"T Ma","year":"1998","unstructured":"Ma, T. et al. Severely impaired urinary concentrating ability in transgenic mice lacking aquaporin-1 water channels. J. Biol. Chem. 273, 4296\u20134299. https:\/\/doi.org\/10.1074\/jbc.273.8.4296 (1998).","journal-title":"J. Biol. Chem."},{"key":"81324_CR4","doi-asserted-by":"publisher","first-page":"2775","DOI":"10.1074\/jbc.M008216200","volume":"276","author":"B Yang","year":"2001","unstructured":"Yang, B., Gillespie, A., Carlson, E. J., Epstein, C. J. & Verkman, A. S. Neonatal mortality in an aquaporin-2 knock-in mouse model of recessive nephrogenic diabetes insipidus. J. Biol. Chem. 276, 2775\u20132779. https:\/\/doi.org\/10.1074\/jbc.M008216200 (2001).","journal-title":"J. Biol. Chem."},{"key":"81324_CR5","doi-asserted-by":"publisher","first-page":"4386","DOI":"10.1073\/pnas.080499597","volume":"97","author":"T Ma","year":"2000","unstructured":"Ma, T. et al. Nephrogenic diabetes insipidus in mice lacking aquaporin-3 water channels. Proc. Natl. Acad. Sci. U S A. 97, 4386\u20134391. https:\/\/doi.org\/10.1073\/pnas.080499597 (2000).","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"81324_CR6","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1007\/s13730-022-00723-0","volume":"12","author":"K Kakeshita","year":"2023","unstructured":"Kakeshita, K., Imamura, T., Onoda, H. & Kinugawa, K. Impact of Goreisan upon aquaporin-2-incorporated aquaresis system in patients with congestive heart failure. CEN Case Rep. 12, 73\u201377. https:\/\/doi.org\/10.1007\/s13730-022-00723-0 (2023).","journal-title":"CEN case Rep."},{"key":"81324_CR7","doi-asserted-by":"publisher","DOI":"10.1007\/s00380-024-02432-3","author":"Y Hida","year":"2024","unstructured":"Hida, Y., Imamura, T., Onoda, H., Kakeshita, K. & Kinugawa, K. Short-term impact of kampo goreisan in patients with congestive heart failure refractory to tolvaptan-incorporated medical therapy. Heart Vessels. https:\/\/doi.org\/10.1007\/s00380-024-02432-3 (2024).","journal-title":"Heart Vessels"},{"key":"81324_CR8","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.ahj.2023.02.013","volume":"260","author":"H Yaku","year":"2023","unstructured":"Yaku, H. et al. Rationale and study design of the GOREISAN for heart failure (GOREISAN-HF) trial: A randomized clinical trial. Am. Heart J. 260, 18\u201325. https:\/\/doi.org\/10.1016\/j.ahj.2023.02.013 (2023).","journal-title":"Am. Heart J."},{"key":"81324_CR9","doi-asserted-by":"publisher","first-page":"145","DOI":"10.11339\/jtm.30.145","volume":"30","author":"M Jo","year":"2013","unstructured":"Jo, M., Fujimoto, T., Kaneko, M., Oka, H. & Shibahara, N. Effect of goreisan on urinary concentrating ability and expression of aquaporin-2 in 5\/6 nephrectomized rats. J. Tradit. Med. 30, 145\u2013157. https:\/\/doi.org\/10.11339\/jtm.30.145 (2013).","journal-title":"J. Traditional Med."},{"key":"81324_CR10","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.jphs.2023.07.001","volume":"153","author":"A Suenaga","year":"2023","unstructured":"Suenaga, A. et al. TJ-17 (Goreisan) mitigates renal fibrosis in a mouse model of folic acid-induced chronic kidney disease. J. Pharmacol. Sci. 153, 31\u201337. https:\/\/doi.org\/10.1016\/j.jphs.2023.07.001 (2023).","journal-title":"J. Pharmacol. Sci."},{"key":"81324_CR11","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1002\/tkm2.1276","volume":"8","author":"K Murakami","year":"2021","unstructured":"Murakami, K. et al. Goreisan regulates AQP3 expression and improves diarrhea. Tradit. Kampo Med. 8, 91\u201399. https:\/\/doi.org\/10.1002\/tkm2.1276 (2021).","journal-title":"Traditional Kampo Med."},{"key":"81324_CR12","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1002\/tkm2.1380","volume":"10","author":"T Shimizu","year":"2023","unstructured":"Shimizu, T., Murakami, K., Matsumoto, C., Kido, T. & Isohama, Y. Goreisan alleviates cerebral edema: possibility of its involvement in inhibiting aquaporin-4 function. Tradit. Kampo Med. 10, 168\u2013176. https:\/\/doi.org\/10.1002\/tkm2.1380 (2023).","journal-title":"Traditional Kampo Med."},{"key":"81324_CR13","doi-asserted-by":"publisher","first-page":"1712","DOI":"10.1038\/ki.1996.253","volume":"49","author":"MA Knepper","year":"1996","unstructured":"Knepper, M. A. et al. Renal aquaporins. Kidney Int. 49, 1712\u20131717. https:\/\/doi.org\/10.1038\/ki.1996.253 (1996).","journal-title":"Kidney Int."},{"key":"81324_CR14","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1038\/s41581-021-00447-x","volume":"17","author":"ETB Olesen","year":"2021","unstructured":"Olesen, E. T. B. & Fenton, R. A. Aquaporin 2 regulation: implications for water balance and polycystic kidney diseases. Nat. Rev. Nephrol. 17, 765\u2013781. https:\/\/doi.org\/10.1038\/s41581-021-00447-x (2021).","journal-title":"Nat. Rev. Nephrol."},{"key":"81324_CR15","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1038\/ki.2008.505","volume":"75","author":"HB Moeller","year":"2009","unstructured":"Moeller, H. B., Knepper, M. A. & Fenton, R. A. Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells. Kidney Int. 75, 295\u2013303. https:\/\/doi.org\/10.1038\/ki.2008.505 (2009).","journal-title":"Kidney Int."},{"key":"81324_CR16","doi-asserted-by":"publisher","first-page":"e2202125119","DOI":"10.1073\/pnas.2202125119","volume":"119","author":"Y Hara","year":"2022","unstructured":"Hara, Y. et al. LRBA is essential for urinary concentration and body water homeostasis. Proc. Natl. Acad. Sci. U S A. 119, e2202125119. https:\/\/doi.org\/10.1073\/pnas.2202125119 (2022).","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"81324_CR17","doi-asserted-by":"publisher","first-page":"F1018","DOI":"10.1152\/ajprenal.00580.2009","volume":"298","author":"L Xie","year":"2010","unstructured":"Xie, L. et al. Quantitative analysis of aquaporin-2 phosphorylation. Am. J. Physiol.-Renal Physiol. 298, F1018\u2013F1023. https:\/\/doi.org\/10.1152\/ajprenal.00580.2009 (2010).","journal-title":"Am. J. Physiology-Renal Physiol."},{"key":"81324_CR18","doi-asserted-by":"publisher","first-page":"2725","DOI":"10.1038\/nprot.2014.181","volume":"9","author":"RH Giles","year":"2014","unstructured":"Giles, R. H., Ajzenberg, H. & Jackson, P. K. 3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders. Nat. Protoc. 9, 2725\u20132731. https:\/\/doi.org\/10.1038\/nprot.2014.181 (2014).","journal-title":"Nat. Protoc."},{"key":"81324_CR19","doi-asserted-by":"publisher","first-page":"e0131719","DOI":"10.1371\/journal.pone.0131719","volume":"10","author":"WL Rice","year":"2015","unstructured":"Rice, W. L. et al. Polarized trafficking of AQP2 revealed in three dimensional epithelial culture. PloS One. 10, e0131719. https:\/\/doi.org\/10.1371\/journal.pone.0131719 (2015).","journal-title":"PloS One"},{"key":"81324_CR20","doi-asserted-by":"publisher","unstructured":"Noda, Y. & Sasaki, S. Updates and perspectives on aquaporin-2 and water balance disorders. Int. J. Mol. Sci. 22. https:\/\/doi.org\/10.3390\/ijms222312950 (2021).","DOI":"10.3390\/ijms222312950"},{"key":"81324_CR21","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1016\/j.semnephrol.2008.03.006","volume":"28","author":"AJM Loonen","year":"2008","unstructured":"Loonen, A. J. M., Knoers, N. V. A. M., van Os, C. H. & Deen, P. M. T. Aquaporin 2 mutations in nephrogenic diabetes insipidus. Semin. Nephrol. 28, 252\u2013265. https:\/\/doi.org\/10.1016\/j.semnephrol.2008.03.006 (2008).","journal-title":"Semin. Nephrol."},{"key":"81324_CR22","doi-asserted-by":"publisher","first-page":"24617","DOI":"10.1074\/jbc.M803074200","volume":"283","author":"JD Hoffert","year":"2008","unstructured":"Hoffert, J. D. et al. Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J. Biol. Chem. 283, 24617\u201324627. https:\/\/doi.org\/10.1074\/jbc.M803074200 (2008).","journal-title":"J. Biol. Chem."},{"key":"81324_CR23","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1093\/brain\/124.2.369","volume":"124","author":"BS Athwal","year":"2001","unstructured":"Athwal, B. S. et al. Brain responses to changes in bladder volume and urge to void in healthy men. Brain: J. Neurol. 124, 369\u2013377. https:\/\/doi.org\/10.1093\/brain\/124.2.369 (2001).","journal-title":"Brain: J. Neurol."},{"key":"81324_CR24","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1038\/nrn2401","volume":"9","author":"CJ Fowler","year":"2008","unstructured":"Fowler, C. J., Griffiths, D. & de Groat, W. C. The neural control of micturition. Nat. Rev. Neurosci. 9, 453\u2013466. https:\/\/doi.org\/10.1038\/nrn2401 (2008).","journal-title":"Nat. Rev. Neurosci."},{"key":"81324_CR25","doi-asserted-by":"publisher","first-page":"30","DOI":"10.3923\/jms.2011.30.38","volume":"11","author":"T Kurita","year":"2011","unstructured":"Kurita, T. et al. Effects of Gorei-san: A traditional Japanese Kampo medicine, on aquaporin 1, 2, 3, 4 and V2R mRNA expression in rat kidney and forebrain. J. Med. Sci. 11, 30\u201338. https:\/\/doi.org\/10.3923\/jms.2011.30.38 (2011).","journal-title":"J. Med. Sci."},{"key":"81324_CR26","doi-asserted-by":"crossref","unstructured":"Bogum, J. et al. Small-molecule screening identifies modulators of aquaporin-2 trafficking. J. Am. Soc. Nephrol. 24 (2013).","DOI":"10.1681\/ASN.2012030295"},{"key":"81324_CR27","doi-asserted-by":"publisher","first-page":"18344","DOI":"10.1073\/pnas.0604073103","volume":"103","author":"EJ Kamsteeg","year":"2006","unstructured":"Kamsteeg, E. J. et al. Short-chain ubiquitination mediates the regulated endocytosis of the aquaporin-2 water channel. Proc. Natl. Acad. Sci. 103, 18344\u201318349 (2006).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"81324_CR28","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.jep.2016.03.030","volume":"185","author":"YJ Lee","year":"2016","unstructured":"Lee, Y. J. et al. Quantitative evaluation of oryeongsan and its action on water regulation in renal inner medullary collecting duct cells. J. Ethnopharmacol. 185, 310\u2013318. https:\/\/doi.org\/10.1016\/j.jep.2016.03.030 (2016).","journal-title":"J. Ethnopharmacol."},{"key":"81324_CR29","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1055\/s-2002-23129","volume":"68","author":"B Makino","year":"2002","unstructured":"Makino, B. et al. Local variation in the content of angiotensin II and arginine vasopressin receptor antagonistic terpenoids in the rhizomes of Alisma Orientale. Planta Med. 68, 226\u2013231. https:\/\/doi.org\/10.1055\/s-2002-23129 (2002).","journal-title":"Planta Med."},{"key":"81324_CR30","doi-asserted-by":"publisher","first-page":"1399","DOI":"10.1172\/jci119299","volume":"99","author":"JM Sands","year":"1997","unstructured":"Sands, J. M. et al. Apical extracellular calcium\/polyvalent cation-sensing receptor regulates vasopressin-elicited water permeability in rat kidney inner medullary collecting duct. J. Clin. Investig. 99, 1399\u20131405. https:\/\/doi.org\/10.1172\/jci119299 (1997).","journal-title":"J. Clin. Investig."},{"key":"81324_CR31","doi-asserted-by":"publisher","first-page":"2245","DOI":"10.1111\/j.1523-1755.2004.66036.x","volume":"66","author":"G Procino","year":"2004","unstructured":"Procino, G. et al. Extracellular calcium antagonizes forskolin-induced aquaporin 2 trafficking in collecting duct cells. Kidney Int. 66, 2245\u20132255. https:\/\/doi.org\/10.1111\/j.1523-1755.2004.66036.x (2004).","journal-title":"Kidney Int."},{"key":"81324_CR32","doi-asserted-by":"publisher","first-page":"1524S","DOI":"10.1093\/jn\/137.6.1524S","volume":"137","author":"AD Conigrave","year":"2007","unstructured":"Conigrave, A. D., Mun, H. C. & Lok, H. C. Aromatic l-amino acids activate the calcium-sensing receptor123. J. Nutr. 137, 1524S-1527S. https:\/\/doi.org\/10.1093\/jn\/137.6.1524S (2007).","journal-title":"J. Nutr."},{"key":"81324_CR33","doi-asserted-by":"publisher","first-page":"F978","DOI":"10.1152\/ajprenal.1998.274.5.F978","volume":"274","author":"M Sands","year":"1998","unstructured":"Sands, M. et al. Vasopressin-elicited water and urea permeabilities are altered in IMCD in hypercalcemic rats. Am. J. Physiol.-Renal Physiol. 274, F978\u2013F985. https:\/\/doi.org\/10.1152\/ajprenal.1998.274.5.F978 (1998).","journal-title":"Am. J. Physiology-Renal Physiol."},{"key":"81324_CR34","doi-asserted-by":"publisher","first-page":"1873","DOI":"10.1681\/asn.V11101873","volume":"11","author":"G Valenti","year":"2000","unstructured":"Valenti, G. et al. Urinary aquaporin 2 and calciuria correlate with the severity of enuresis in children. J. Am. Soc. Nephrol.: JASN. 11, 1873\u20131881. https:\/\/doi.org\/10.1681\/asn.V11101873 (2000).","journal-title":"J. Am. Soc. Nephrology: JASN"},{"key":"81324_CR35","doi-asserted-by":"publisher","first-page":"F895","DOI":"10.1152\/ajprenal.00354.2001","volume":"283","author":"G Valenti","year":"2002","unstructured":"Valenti, G. et al. Low-calcium diet in hypercalciuric enuretic children restores AQP2 excretion and improves clinical symptoms. Am. J. Physiol.-Renal Physiol. 283, F895\u2013F903. https:\/\/doi.org\/10.1152\/ajprenal.00354.2001 (2002).","journal-title":"Am. J. Physiology-Renal Physiol."},{"key":"81324_CR36","doi-asserted-by":"publisher","first-page":"2350","DOI":"10.1242\/jcs.168096","volume":"128","author":"M Ranieri","year":"2015","unstructured":"Ranieri, M. et al. Negative feedback from CaSR signaling to aquaporin-2 sensitizes vasopressin to extracellular Ca2. J. Cell. Sci. 128, 2350\u20132360. https:\/\/doi.org\/10.1242\/jcs.168096 (2015).","journal-title":"J. Cell. Sci."},{"key":"81324_CR37","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1038\/366575a0","volume":"366","author":"EM Brown","year":"1993","unstructured":"Brown, E. M. et al. Cloning and characterization of an extracellular ca(2+)-sensing receptor from bovine parathyroid. Nature 366, 575\u2013580. https:\/\/doi.org\/10.1038\/366575a0 (1993).","journal-title":"Nature"},{"key":"81324_CR38","doi-asserted-by":"publisher","first-page":"4040","DOI":"10.1073\/pnas.95.7.4040","volume":"95","author":"EF Nemeth","year":"1998","unstructured":"Nemeth, E. F. et al. Calcimimetics with potent and selective activity on the parathyroid calcium receptor. Proc. Natl. Acad. Sci. U S A 95, 4040\u20134045. https:\/\/doi.org\/10.1073\/pnas.95.7.4040 (1998).","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"81324_CR39","doi-asserted-by":"publisher","first-page":"2148","DOI":"10.1096\/fj.201700412RR","volume":"32","author":"M Ranieri","year":"2018","unstructured":"Ranieri, M. et al. CaSR signaling down-regulates AQP2 expression via a novel microRNA pathway in pendrin and NaCl cotransporter knockout mice. FASEB J. Off. Publ. Fed. Am. Soc. Exp. Biol. 32, 2148\u20132159. https:\/\/doi.org\/10.1096\/fj.201700412RR (2018).","journal-title":"FASEB Journal: Official Publication Federation Am. Soc. Experimental Biology"},{"key":"81324_CR40","doi-asserted-by":"publisher","first-page":"4537","DOI":"10.1038\/s41598-021-83850-5","volume":"11","author":"M Ranieri","year":"2021","unstructured":"Ranieri, M. et al. Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor. Sci. Rep. 11, 4537. https:\/\/doi.org\/10.1038\/s41598-021-83850-5 (2021).","journal-title":"Sci. Rep."},{"key":"81324_CR41","doi-asserted-by":"publisher","first-page":"123409","DOI":"10.1016\/j.jchromb.2022.123409","volume":"1208","author":"R Xu","year":"2022","unstructured":"Xu, R. et al. Extracellular domain of human calcium sensing receptor immobilized to silica beads as biomaterial: A rapid chromatographic method for recognizing ligands from complex matrix \u2018Shuangdan\u2019. J. Chromatogr. B. 1208, 123409. https:\/\/doi.org\/10.1016\/j.jchromb.2022.123409 (2022).","journal-title":"J. Chromatogr. B"},{"key":"81324_CR42","doi-asserted-by":"publisher","first-page":"112829","DOI":"10.1016\/j.biopha.2022.112829","volume":"149","author":"J Zhang","year":"2022","unstructured":"Zhang, J. et al. Screening out key compounds of glechomae herba for antiurolithic activity and quality control based on spectrum-effect relationships coupled with UPLC-QDA. Biomed. Pharmacother. 149, 112829. https:\/\/doi.org\/10.1016\/j.biopha.2022.112829 (2022).","journal-title":"Biomed. Pharmacother."},{"key":"81324_CR43","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1007\/s00604-023-06092-4","volume":"191","author":"P Cheng","year":"2023","unstructured":"Cheng, P. et al. Solid-phase extraction with the functionalization of calcium-sensing receptors onto magnetic microspheres as an affinity probe can capture ligands selectively from herbal extract. Microchim. Acta. 191, 34. https:\/\/doi.org\/10.1007\/s00604-023-06092-4 (2023).","journal-title":"Microchim. Acta"},{"key":"81324_CR44","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1248\/bpb.b23-00829","volume":"47","author":"Z Kawakami","year":"2024","unstructured":"Kawakami, Z. et al. Effect of Goreisan, a traditional Japanese medicine, on rat hindlimb lymphedema. Biol. Pharm. Bull. 47, 1179\u20131188. https:\/\/doi.org\/10.1248\/bpb.b23-00829 (2024).","journal-title":"Biol. Pharm. Bull."},{"key":"81324_CR45","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s11418-021-01515-z","volume":"75","author":"S Sanechika","year":"2021","unstructured":"Sanechika, S., Shimobori, C. & Ohbuchi, K. Identification of herbal components as TRPA1 agonists and TRPM8 antagonists. J. Nat. Med. 75, 717\u2013725. https:\/\/doi.org\/10.1007\/s11418-021-01515-z (2021).","journal-title":"J. Nat. Med."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-81324-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-81324-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-81324-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T15:04:19Z","timestamp":1732806259000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-81324-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,28]]},"references-count":45,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["81324"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-81324-y","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4398285\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,28]]},"assertion":[{"value":"10 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All authors are employed by Tsumura & Co. This study received funding from Tsumura & Co. The funder was involved in the following aspects of the study: study design, data collection and analysis, publication decision, and manuscript preparation. The authors declare no other competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"All animal experiments were approved by the Laboratory Animal Committee of Tsumura & Co. (approval nos. 23\u2009\u2212\u2009013, 23\u2013024) and performed in accordance with guidelines for the conduct of animal experiments from the Ministry of Health, Labour and Welfare of Japan. This study is reported in accordance with ARRIVE guidelines.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}],"article-number":"29650"}}