{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T16:34:38Z","timestamp":1775147678176,"version":"3.50.1"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T00:00:00Z","timestamp":1732838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T00:00:00Z","timestamp":1732838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"This research was financially supported by grants from the Key R&D Project of Science and Technology Development Plan of Jilin Provincial Department of Science and Technology"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-81405-y","type":"journal-article","created":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T10:29:55Z","timestamp":1732876195000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mycobacterium tuberculosis Rv1048c affects the biological characteristics of recombinant Mycobacterium smegmatis"],"prefix":"10.1038","volume":"14","author":[{"given":"Dan-Ni","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Yue","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Biao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Ming","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nai-Chao","family":"Diao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan-Li","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,11,29]]},"reference":[{"key":"81405_CR1","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1111\/jam.14478","volume":"128","author":"R Singh","year":"2020","unstructured":"Singh, R. et al. Recent updates on drug resistance in Mycobacterium tuberculosis. J. Appl. Microbiol. 128, 1547\u20131567 (2020).","journal-title":"J. Appl. Microbiol."},{"key":"81405_CR2","unstructured":"WHO, Global tuberculosis reports 2022[EB\/OL]. (Accessed 28 Oct 2023) (World Health Organization, 2022)."},{"key":"81405_CR3","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.870768","volume":"13","author":"KA Yusoof","year":"2022","unstructured":"Yusoof, K. A. et al. Tuberculosis phenotypic and genotypic drug susceptibility testing and immunodiagnostics: a review. Front. Immunol. 13, 870768 (2022).","journal-title":"Front. Immunol."},{"key":"81405_CR4","unstructured":"A. Zweynert, Drug-Resistant TB Threatens to Kill 75 Million People by 2050, Cost $16.7 Trillion. (2022)."},{"issue":"2021","key":"81405_CR5","first-page":"427","volume":"70","author":"R Fukunaga","year":"2019","unstructured":"Fukunaga, R. et al. Epidemiology of tuberculosis and progress toward meeting global targets - worldwide. MMWR Morb. Mortal. Wkly. Rep. 70(2021), 427\u2013430 (2019).","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"issue":"2020","key":"81405_CR6","first-page":"281","volume":"69","author":"A MacNeil","year":"2018","unstructured":"MacNeil, A. et al. Global epidemiology of tuberculosis and progress toward meeting global targets - worldwide. MMWR Morb. Mortal. Wkly. Rep. 69(2020), 281\u2013285 (2018).","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"81405_CR7","first-page":"90","volume":"91","author":"VJ Gergert","year":"2019","unstructured":"Gergert, V. J., Averbakh, M. M. & Ergeshov, A. E. Immunological aspects of tuberculosis pathogenesis. Ter Arkh 91, 90\u201397 (2019).","journal-title":"Ter Arkh"},{"key":"81405_CR8","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.chom.2008.05.006","volume":"3","author":"J Pieters","year":"2008","unstructured":"Pieters, J. Mycobacterium tuberculosis and the macrophage: maintaining a balance. Cell Host Microbe 3, 399\u2013407 (2008).","journal-title":"Cell Host Microbe"},{"key":"81405_CR9","doi-asserted-by":"publisher","first-page":"42225","DOI":"10.1038\/srep42225","volume":"7","author":"N Andreu","year":"2017","unstructured":"Andreu, N. et al. Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis. Sci. Rep. 7, 42225 (2017).","journal-title":"Sci. Rep."},{"key":"81405_CR10","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/747543","volume":"2015","author":"JK Sia","year":"2015","unstructured":"Sia, J. K., Georgieva, M. & Rengarajan, J. Innate immune defenses in human tuberculosis: an overview of the interactions between Mycobacterium tuberculosis and innate immune cells. J. Immunol. Res. 2015, 747543 (2015).","journal-title":"J. Immunol. Res."},{"key":"81405_CR11","doi-asserted-by":"publisher","first-page":"4376","DOI":"10.1073\/pnas.0711159105","volume":"105","author":"AK Pandey","year":"2008","unstructured":"Pandey, A. K. & Sassetti, C. M. Mycobacterial persistence requires the utilization of host cholesterol. Proc. Natl. Acad. Sci. USA 105, 4376\u20134380 (2008).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"81405_CR12","doi-asserted-by":"publisher","first-page":"1734","DOI":"10.1111\/j.1462-5822.2012.01834.x","volume":"14","author":"D Portevin","year":"2012","unstructured":"Portevin, D., Via, L. E., Eum, S. & Young, D. Natural killer cells are recruited during pulmonary tuberculosis and their ex vivo responses to mycobacteria vary between healthy human donors in association with KIR haplotype. Cell Microbiol. 14, 1734\u20131744 (2012).","journal-title":"Cell Microbiol."},{"key":"81405_CR13","doi-asserted-by":"publisher","first-page":"2967","DOI":"10.1099\/00221287-148-10-2967","volume":"148","author":"JC Camus","year":"2002","unstructured":"Camus, J. C., Pryor, M. J., Medigue, C. & Cole, S. T. Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv. Microbiology (Reading) 148, 2967\u20132973 (2002).","journal-title":"Microbiology (Reading)"},{"key":"81405_CR14","doi-asserted-by":"publisher","first-page":"1488","DOI":"10.1016\/j.cmi.2020.07.036","volume":"26","author":"T Schon","year":"2020","unstructured":"Schon, T. et al. Antimicrobial susceptibility testing of Mycobacterium tuberculosis complex isolates - the EUCAST broth microdilution reference method for MIC determination. Clin. Microbiol. Infect. 26, 1488\u20131492 (2020).","journal-title":"Clin. Microbiol. Infect."},{"key":"81405_CR15","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402\u2013408 (2001).","journal-title":"Methods"},{"key":"81405_CR16","unstructured":"Bloom, B. R. (eds) Major Infectious Diseases (2017)."},{"key":"81405_CR17","doi-asserted-by":"publisher","first-page":"1697","DOI":"10.1080\/21645515.2018.1458806","volume":"14","author":"W Gong","year":"2018","unstructured":"Gong, W., Liang, Y. & Wu, X. The current status, challenges, and future developments of new tuberculosis vaccines. Hum. Vaccin. Immunother. 14, 1697\u20131716 (2018).","journal-title":"Hum. Vaccin. Immunother."},{"key":"81405_CR18","doi-asserted-by":"publisher","first-page":"10-1128","DOI":"10.1128\/microbiolspec.TBTB2-0025-2016","volume":"4","author":"BC VanderVen","year":"2016","unstructured":"VanderVen, B. C., Huang, L., Rohde, K. H. & Russell, D. G. The minimal unit of infection: Mycobacterium tuberculosis in the macrophage. Microbiol. Spectr. 4, 10\u20131128 (2016).","journal-title":"Microbiol. Spectr."},{"key":"81405_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2023.106974","volume":"246","author":"D Zhao","year":"2023","unstructured":"Zhao, D. et al. Mycobacterium tuberculosis Rv3435c regulates inflammatory cytokines and promotes the intracellular survival of recombinant Mycobacteria. Acta Trop. 246, 106974 (2023).","journal-title":"Acta Trop."},{"key":"81405_CR20","doi-asserted-by":"publisher","first-page":"C1444","DOI":"10.1152\/ajpcell.00246.2022","volume":"323","author":"A Nisa","year":"2022","unstructured":"Nisa, A. et al. Different modalities of host cell death and their impact on Mycobacterium tuberculosis infection. Am. J. Physiol. Cell Physiol. 323, C1444\u2013C1474 (2022).","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"81405_CR21","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1093\/femsre\/fuz006","volume":"43","author":"C Bussi","year":"2019","unstructured":"Bussi, C. & Gutierrez, M. G. Mycobacterium tuberculosis infection of host cells in space and time. FEMS Microbiol. Rev. 43, 341\u2013361 (2019).","journal-title":"FEMS Microbiol. Rev."},{"key":"81405_CR22","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.1016\/j.jiph.2020.06.023","volume":"13","author":"J Ranjitha","year":"2020","unstructured":"Ranjitha, J., Rajan, A. & Shankar, V. Features of the biochemistry of Mycobacterium smegmatis, as a possible model for Mycobacterium tuberculosis. J. Infect. Public Health 13, 1255\u20131264 (2020).","journal-title":"J. Infect. Public Health"},{"key":"81405_CR23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/pro.2584","volume":"24","author":"G Bashiri","year":"2015","unstructured":"Bashiri, G. & Baker, E. N. Production of recombinant proteins in Mycobacterium smegmatis for structural and functional studies. Protein Sci. 24, 1\u201310 (2015).","journal-title":"Protein Sci."},{"key":"81405_CR24","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1002\/emmm.201000079","volume":"2","author":"MJ Kim","year":"2010","unstructured":"Kim, M. J. et al. Caseation of human tuberculosis granulomas correlates with elevated host lipid metabolism. EMBO Mol. Med. 2, 258\u2013274 (2010).","journal-title":"EMBO Mol. Med."},{"key":"81405_CR25","doi-asserted-by":"publisher","first-page":"S42","DOI":"10.1016\/j.tube.2019.04.010","volume":"116S","author":"ME Gough","year":"2019","unstructured":"Gough, M. E., Graviss, E. A., Chen, T. A., Obasi, E. M. & May, E. E. Compounding effect of vitamin D(3) diet, supplementation, and alcohol exposure on macrophage response to mycobacterium infection. Tuberculosis 116S, S42\u2013S58 (2019).","journal-title":"Tuberculosis"},{"key":"81405_CR26","doi-asserted-by":"publisher","first-page":"39121","DOI":"10.1039\/D0RA08122A","volume":"10","author":"YS Lee","year":"2020","unstructured":"Lee, Y. S. et al. Cycling system for decomposition of gaseous benzene by hydrogen peroxide with naturally Fe-containing activated carbon. RSC Adv. 10, 39121\u201339129 (2020).","journal-title":"RSC Adv."},{"key":"81405_CR27","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1016\/j.chemosphere.2017.05.039","volume":"182","author":"A Fischbacher","year":"2017","unstructured":"Fischbacher, A., von Sonntag, C. & Schmidt, T. C. Hydroxyl radical yields in the Fenton process under various pH, ligand concentrations and hydrogen peroxide\/Fe(II) ratios. Chemosphere 182, 738\u2013744 (2017).","journal-title":"Chemosphere"},{"key":"81405_CR28","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.chemphyslip.2006.02.017","volume":"141","author":"AG Serrano","year":"2006","unstructured":"Serrano, A. G. & Perez-Gil, J. Protein-lipid interactions and surface activity in the pulmonary surfactant system. Chem. Phys. Lipids 141, 105\u2013118 (2006).","journal-title":"Chem. Phys. Lipids"},{"key":"81405_CR29","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1080\/1061186X.2020.1853733","volume":"29","author":"X Xiang","year":"2021","unstructured":"Xiang, X., Gong, Z., Deng, W., Sun, Q. & Xie, J. Mycobacterial ethambutol responsive genes and implications in antibiotics resistance. J. Drug Target 29, 284\u2013293 (2021).","journal-title":"J. Drug Target"},{"issue":"Suppl 3","key":"81405_CR30","doi-asserted-by":"publisher","first-page":"S107","DOI":"10.1093\/infdis\/jiv009","volume":"211","author":"V Sahasrabudhe","year":"2015","unstructured":"Sahasrabudhe, V., Zhu, T., Vaz, A. & Tse, S. Drug metabolism and drug interactions: potential application to antituberculosis drugs. J. Infect. Dis. 211(Suppl 3), S107-114 (2015).","journal-title":"J. Infect. Dis."},{"key":"81405_CR31","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00592-22","volume":"66","author":"S Shee","year":"2022","unstructured":"Shee, S. et al. Moxifloxacin-mediated killing of Mycobacterium tuberculosis involves respiratory downshift, reductive stress, and accumulation of reactive oxygen species. Antimicrob. Agents Chemother. 66, e0059222 (2022).","journal-title":"Antimicrob. Agents Chemother."},{"key":"81405_CR32","doi-asserted-by":"publisher","first-page":"9192","DOI":"10.1074\/jbc.RA120.012809","volume":"295","author":"K Sachdeva","year":"2020","unstructured":"Sachdeva, K. et al. Mycobacterium tuberculosis (Mtb) lipid mediated lysosomal rewiring in infected macrophages modulates intracellular Mtb trafficking and survival. J. Biol. Chem. 295, 9192\u20139210 (2020).","journal-title":"J. Biol. Chem."},{"key":"81405_CR33","doi-asserted-by":"publisher","first-page":"853","DOI":"10.2217\/fmb.15.11","volume":"10","author":"K Hussain Bhat","year":"2015","unstructured":"Hussain Bhat, K. & Mukhopadhyay, S. Macrophage takeover and the host-bacilli interplay during tuberculosis. Future Microbiol. 10, 853\u2013872 (2015).","journal-title":"Future Microbiol."},{"key":"81405_CR34","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1002\/eji.200535496","volume":"36","author":"A Kahnert","year":"2006","unstructured":"Kahnert, A. et al. Alternative activation deprives macrophages of a coordinated defense program to Mycobacterium tuberculosis. Eur. J. Immunol. 36, 631\u2013647 (2006).","journal-title":"Eur. J. Immunol."},{"key":"81405_CR35","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/fty037","author":"S Upadhyay","year":"2018","unstructured":"Upadhyay, S., Mittal, E. & Philips, J. A. Tuberculosis and the art of macrophage manipulation. Pathog. Dis. https:\/\/doi.org\/10.1093\/femspd\/fty037 (2018).","journal-title":"Pathog. Dis."},{"key":"81405_CR36","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1002\/JLB.MR0318-095RR","volume":"106","author":"A Khan","year":"2019","unstructured":"Khan, A., Singh, V. K., Hunter, R. L. & Jagannath, C. Macrophage heterogeneity and plasticity in tuberculosis. J. Leukoc. Biol. 106, 275\u2013282 (2019).","journal-title":"J. Leukoc. Biol."},{"key":"81405_CR37","doi-asserted-by":"publisher","first-page":"17576","DOI":"10.1074\/jbc.M113.545715","volume":"289","author":"E Garcia-Fernandez","year":"2014","unstructured":"Garcia-Fernandez, E., Medrano, F. J., Galan, B. & Garcia, J. L. Deciphering the transcriptional regulation of cholesterol catabolic pathway in mycobacteria: identification of the inducer of KstR repressor. J. Biol. Chem. 289, 17576\u201317588 (2014).","journal-title":"J. Biol. Chem."},{"key":"81405_CR38","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1016\/j.chembiol.2011.12.016","volume":"19","author":"JE Griffin","year":"2012","unstructured":"Griffin, J. E. et al. Cholesterol catabolism by Mycobacterium tuberculosis requires transcriptional and metabolic adaptations. Chem. Biol. 19, 218\u2013227 (2012).","journal-title":"Chem. Biol."},{"key":"81405_CR39","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1111\/imr.12266","volume":"264","author":"G Weiss","year":"2015","unstructured":"Weiss, G. & Schaible, U. E. Macrophage defense mechanisms against intracellular bacteria. Immunol. Rev. 264, 182\u2013203 (2015).","journal-title":"Immunol. Rev."},{"key":"81405_CR40","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2019.104070","volume":"77","author":"C Ruan","year":"2020","unstructured":"Ruan, C. et al. Mycobacterium tuberculosis Rv0426c promotes recombinant mycobacteria intracellular survival via manipulating host inflammatory cytokines and suppressing cell apoptosis. Infect. Genet. Evol. 77, 104070 (2020).","journal-title":"Infect. Genet. Evol."},{"key":"81405_CR41","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1111\/imr.12268","volume":"264","author":"Z Hmama","year":"2015","unstructured":"Hmama, Z., Pena-Diaz, S., Joseph, S. & Av-Gay, Y. Immunoevasion and immunosuppression of the macrophage by Mycobacterium tuberculosis. Immunol. Rev. 264, 220\u2013232 (2015).","journal-title":"Immunol. Rev."},{"key":"81405_CR42","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1186\/2193-1801-2-686","volume":"2","author":"PP Singh","year":"2013","unstructured":"Singh, P. P. & Goyal, A. Interleukin-6: a potent biomarker of mycobacterial infection. Springerplus 2, 686 (2013).","journal-title":"Springerplus"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-81405-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-81405-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-81405-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T11:05:47Z","timestamp":1732878347000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-81405-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,29]]},"references-count":42,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["81405"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-81405-y","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4383590\/v1","asserted-by":"object"}]},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,29]]},"assertion":[{"value":"7 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 November 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"29749"}}