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From an ENU-mutated mangrove killifish line R228, we have isolated a novel mutant line, no-fin-ray\/<jats:italic>nfr<\/jats:italic> in which homozygous mutant of adult fish fin ray development is largely reduced. Illumina RNAseq with 3 embryos each from mutants, siblings and the parental WT strain Hon9 (only 9 embryos as total) identified a mutation in the <jats:italic>edaradd<\/jats:italic> in a highly conserved C-terminal death domain. Edaradd is known as a cytoplasmic accessory protein for the Ectodysplasin A (<jats:italic>EDA<\/jats:italic>) signalling pathway. To confirm the crucial role of <jats:italic>edaradd<\/jats:italic> during fin development, CRISPR RNAs were designed to suppress the gene in another killifish species, Arabian killifish. Indeed, Arabian killifish <jats:italic>edaradd<\/jats:italic> crispants showed a potent reduction of the fin development with 100% frequency. Furthermore, <jats:italic>EDA<\/jats:italic> crispants also showed identical phenotypes to that of <jats:italic>edaradd<\/jats:italic> crispants, confirming the fin defect in the mutants\/crispants is caused by the signalling pathway of the <jats:italic>EDA<\/jats:italic> in the killifish species. These data demonstrate a powerful genetic approach using isogenic self-fertilising mangrove killifish as a tool for identifying mutants and their mutations, and revealed the crucial role of <jats:italic>edaradd<\/jats:italic> for the first time in the fish fin development.<\/jats:p>","DOI":"10.1038\/s41598-024-82276-z","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T12:12:19Z","timestamp":1737461539000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Developmental defects in ectodermal appendages caused by missense mutation in edaradd gene in the nfr mangrove killifish kryptolebias marmoratus"],"prefix":"10.1038","volume":"15","author":[{"given":"Hussein A.","family":"Saud","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"O\u2019Neill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian C.","family":"Ring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuhiro","family":"Kudoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"issue":"26","key":"82276_CR1","doi-asserted-by":"publisher","first-page":"9924","DOI":"10.1073\/pnas.0603847103","volume":"103","author":"M Mackiewicz","year":"2006","unstructured":"Mackiewicz, M., Tatarenkov, A., Taylor, D. 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