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It is used in hybridization to develop resistant currant varieties but is on the verge of extinction, listed in Kazakhstan Red Book. This study developed a micropropagation and slow-growth storage protocol for conservation. Genotypes were identified through DNA barcode analysis (\n                    <jats:italic>rbcL<\/jats:italic>\n                    , ITS, and\n                    <jats:italic>matK<\/jats:italic>\n                    ) and sequences uploaded to the National Center for Biotechnology Information database. Genetic diversity was assessed using iPBS primers, generating 98 fragments with 88\u201394% polymorphic bands. Biochemical analysis of fruits showed vitamin C content from 4.64 to 5.61\u00a0mg\/100\u00a0g, vitamin E from 2.26 to 3.16\u00a0mg\/100\u00a0g, vitamin B5 from 3.18 to 4.93\u00a0mg\/100\u00a0g, and quercetin up to 12.5\u00a0mg\/100\u00a0g. Micropropagation stages were optimized with 12% hydrogen peroxide for surface sterilization, achieving up to 73.3% explant viability. Effective hormonal combinations for in vitro culture included WPM with BAP 0.2\u00a0mg L\n                    <jats:sup>\u22121<\/jats:sup>\n                    and GA 0.5\u00a0mg L\n                    <jats:sup>\u22121<\/jats:sup>\n                    , and for propagation, BAP 0.25\u00a0mg L\n                    <jats:sup>\u22121<\/jats:sup>\n                    , GA 0.5\u00a0mg L\n                    <jats:sup>\u22121<\/jats:sup>\n                    , and IBA 0.5\u00a0mg L\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Mannitol (20\u00a0g L\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) was used for slow-growth storage, keeping explants viable for 4\u00a0months without re-cultivation.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-82320-y","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T22:57:29Z","timestamp":1735340249000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["In Vitro conservation and genetic diversity analysis of rare species Ribes janczewskii"],"prefix":"10.1038","volume":"14","author":[{"given":"Aidana","family":"Nurtaza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damira","family":"Dyussembekova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Symbat","family":"Islamova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indira","family":"Samatova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanargul","family":"Zhanybekova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alima","family":"Umirzakova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulmira","family":"Magzumova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Muranets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Almagul","family":"Kakimzhanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,28]]},"reference":[{"key":"82320_CR1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.agrformet.2017.05.004","volume":"243","author":"ED Gutmann","year":"2017","unstructured":"Gutmann, E. 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