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Due to their close contact with human settlements, they are important reservoirs for zoonotic pathogens, such as <jats:italic>Baylisascaris procyonis<\/jats:italic>. The relevance and prevalence of vector-borne pathogens have not yet been fully elucidated. In this study, we screened 285 spleen samples of raccoons, collected between 2019 and 2022 in Germany. The samples were analysed by PCR to detect <jats:italic>Mycoplasma<\/jats:italic> spp., Anaplasmataceae, <jats:italic>Bartonella<\/jats:italic> spp.<jats:italic>, Babesia<\/jats:italic> spp., <jats:italic>Rickettsia<\/jats:italic> spp., Filarioidea, Trypanosomatida and <jats:italic>Hepatozoon<\/jats:italic> spp., and positive PCR products were sequenced. In total, 104 samples were positive for <jats:italic>Mycoplasma<\/jats:italic> spp. (36.49%), making this the first study to detect <jats:italic>Mycoplasma<\/jats:italic> spp. in raccoons outside of North America. Three samples were positive for <jats:italic>Babesia<\/jats:italic> spp. (1.05%) and two for <jats:italic>Anaplasma phagocytophilum<\/jats:italic> (0.7%). Phylogenetic analysis revealed that the <jats:italic>Mycoplasma<\/jats:italic> spp. detected all belong to the haemotrophic mycoplasmas cluster and were grouped within a single phylogenetic clade. Two different <jats:italic>Babesia<\/jats:italic> spp. were detected, one of which was closely related to <jats:italic>Babesia canis<\/jats:italic>, while the other was more closely related to <jats:italic>Babesia<\/jats:italic> sp. from ruminants. It is unclear whether the pathogens detected have an impact on the health of raccoons or whether they may serve as a reservoir for other animals.<\/jats:p>","DOI":"10.1038\/s41598-024-82581-7","type":"journal-article","created":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T04:48:35Z","timestamp":1735361315000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Detection and phylogenetic analysis of blood-associated pathogens from spleen samples of wild raccoons (Procyon lotor) in Germany"],"prefix":"10.1038","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2947-1423","authenticated-orcid":false,"given":"Maria Sophia","family":"Unterk\u00f6fler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aria","family":"Schwingshandl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3812-8383","authenticated-orcid":false,"given":"Barbara","family":"Eigner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3502-4240","authenticated-orcid":false,"given":"Jutta","family":"Pikalo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4915-3943","authenticated-orcid":false,"given":"Josef","family":"Harl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0164-0179","authenticated-orcid":false,"given":"Joachim","family":"Spergser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0376-1606","authenticated-orcid":false,"given":"Peter","family":"Steinbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Jeschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Striese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Striese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann","family":"Ansorge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4178-0133","authenticated-orcid":false,"given":"Hans-Peter","family":"Fuehrer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4536-5508","authenticated-orcid":false,"given":"Mike","family":"Heddergott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,28]]},"reference":[{"key":"82581_CR1","unstructured":"Kaufmann, J. 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