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Therefore, interest has increased in using feed additives to reduce the negative impacts of heat stress on the chickens and improve production performance. This study aimed to assess the effect of supplementing with\n                    <jats:italic>Nigella sativa nanoparticles<\/jats:italic>\n                    (Nano-NS) as an anti-stress and growth promoter in broiler diets under hot climatic conditions. A total of 375 male one-day-old Ross 308 chicks were randomly divided into a control group and four treatment groups (75 chicks\/group). The first group fed a basal diet without additives, the second group fed a basal diet supplemented with avilamycin at 50\u00a0mg\/kg, and the other groups fed a basal diet supplemented with 30, 40, and 50\u00a0mg\/kg Nano-NS, respectively. Despite that feed intake was not affected, feed conversion ratio, body weight gain, and crude protein digestibility improved in broilers fed Nano-NS (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05) compared with avilamycin and the control groups. Adding Nano-NS led to an increase in the dressing percentage and the relative weight of the bursa of Fabricius and thymus. Serum high-density lipoprotein levels increased while total cholesterol and low-density lipoprotein concentrations decreased (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05) in broilers fed Nano-NS compared with control groups. Furthermore, Nano-NS supplementation significantly increased (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05) serum immunoglobulin (IgG and IgA), and superoxide dismutase (SOD) levels, while decreasing malondialdehyde (MDA), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-\u03b1) concentration. Moreover, there was a significant increase in the\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    population and a decrease (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05) in the\n                    <jats:italic>E. coli<\/jats:italic>\n                    and\n                    <jats:italic>C. perfringens<\/jats:italic>\n                    population in chicks fed Nano-NS. In the intestinal tissues, mucin 2 (MUC2) gene expression increased in chickens fed 50\u00a0mg\/kg Nano-NS compared to other groups. It is concluded that adding Nano-NS (up to 50\u00a0mg\/kg) reduced the negative effects of heat stress via enhancing growth performance, immune responses, and antioxidant status, modulating the microbial community structure, and increasing the expression of the MUC2 gene in broilers under high ambient temperature.\n                  <\/jats:p>","DOI":"10.1038\/s41598-024-82725-9","type":"journal-article","created":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T11:35:28Z","timestamp":1736076928000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Dietary Nigella sativa nanoparticles enhance broiler growth performance, antioxidant capacity, immunity, gene expression modulation, and cecal microbiota during high ambient temperatures"],"prefix":"10.1038","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2207-8912","authenticated-orcid":false,"given":"Ahmed M.","family":"Elbaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman S.","family":"Ashmawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A. A.","family":"Farahat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Abdel\u2011Maksoud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimaa A.","family":"Amin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zangabel S.","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,5]]},"reference":[{"issue":"2","key":"82725_CR1","doi-asserted-by":"publisher","first-page":"356","DOI":"10.3390\/ani3020356","volume":"3","author":"LJ Lara","year":"2013","unstructured":"Lara, L. J. & Rostagno, M. H. Impact of heat stress on poultry production. Animals 3 (2), 356\u2013369. https:\/\/doi.org\/10.3390\/ani3020356 (2013).","journal-title":"Animals"},{"key":"82725_CR2","doi-asserted-by":"publisher","unstructured":"Abdel-Moneim, A. M. E. et al. Nutritional manipulation to combat heat stress in poultry\u2013A comprehensive review. Journal of Thermal Biology, 98, p.102915. doi: (2021). https:\/\/doi.org\/10.1016\/j.jtherbio.2021.102915","DOI":"10.1016\/j.jtherbio.2021.102915"},{"key":"82725_CR3","doi-asserted-by":"publisher","unstructured":"Hothersall, B. et al. Thermal nociceptive threshold testing detects altered sensory processing in broiler chickens with spontaneous lameness. PloS one, 9(5), p.e97883. doi: (2014). https:\/\/doi.org\/10.1371\/journal.pone.0097883","DOI":"10.1371\/journal.pone.0097883"},{"issue":"6","key":"82725_CR4","doi-asserted-by":"publisher","first-page":"332","DOI":"10.14737\/journal.aavs\/2016\/4.6.332.341","volume":"4","author":"SS Pawar","year":"2016","unstructured":"Pawar, S. S. et al. Assessing and mitigating the impact of heat stress in poultry. Adv. Anim. Vet. Sci. 4 (6), 332\u2013341. https:\/\/doi.org\/10.14737\/journal.aavs\/2016\/4.6.332.341 (2016).","journal-title":"Adv. Anim. Vet. Sci."},{"key":"82725_CR5","doi-asserted-by":"publisher","unstructured":"Abdel-Moneim, A. M. E., Shehata, A. M., Mohamed, N. G., Elbaz, A. M. & Ibrahim, N. S. Synergistic Effect of spirulina platensis and selenium nanoparticles on growth performance, serum metabolites, immune responses, and antioxidant capacity of heat-stressed broiler chickens. Biol. Trace Elem. Res. 1\u201312. https:\/\/doi.org\/10.1007\/s12011-021-02662-w (2022).","DOI":"10.1007\/s12011-021-02662-w"},{"issue":"1","key":"82725_CR6","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1186\/s12917-024-04050-7","volume":"20","author":"AME Abdel-Moneim","year":"2024","unstructured":"Abdel-Moneim, A. M. E. et al. 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