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Links between bacterial and viral infection and AD\/PD has been suggested in several studies, however, few have attempted to establish a link between fungal infection and AD\/PD. In this study we adopted a nanopore-based sequencing approach to characterise the presence or absence of fungal genera in both human brain tissue and cerebrospinal fluid (CSF). We observed the presence of small fungal burden DNA in two AD brains and a control case (extensive amyloid angiopathy). 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