{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:24:52Z","timestamp":1789467892175,"version":"build-2803163510"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"the Guizhou Science and Technology Department","award":["qiankehejichu-ZK[2022]yiban359"],"award-info":[{"award-number":["qiankehejichu-ZK[2022]yiban359"]}]},{"name":"the Guizhou Science and Technology Department","award":["qiankehejichu-ZK[2022]yiban359"],"award-info":[{"award-number":["qiankehejichu-ZK[2022]yiban359"]}]},{"name":"the Guizhou Science and Technology Department","award":["qiankehejichu-ZK[2024]zhongdian045"],"award-info":[{"award-number":["qiankehejichu-ZK[2024]zhongdian045"]}]},{"name":"the Guizhou Medical University Affiliated Hospital","award":["2021-GMHCT-009"],"award-info":[{"award-number":["2021-GMHCT-009"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No.81960315"],"award-info":[{"award-number":["No.81960315"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No.82360354"],"award-info":[{"award-number":["No.82360354"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research of Common Chronic Diseases","award":["KF-2022-1"],"award-info":[{"award-number":["KF-2022-1"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-024-83244-3","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T15:29:30Z","timestamp":1735572570000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Annexin A5 knockdown inhibits cardiomyocyte apoptosis and alleviates cardiac hypertrophy via activating the PI3K\/AKT\/Bcl-2 signaling pathway"],"prefix":"10.1038","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4508-1273","authenticated-orcid":false,"given":"Lina","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjuan","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanfeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1788-6712","authenticated-orcid":false,"given":"Bei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"83244_CR1","doi-asserted-by":"publisher","first-page":"e153","DOI":"10.1161\/CIR.0000000000001052","volume":"145","author":"CW Tsao","year":"2022","unstructured":"Tsao, C. W. et al. Heart Disease and Stroke Statistics-2022 update: A report from the American Heart Association. Circulation 145, e153\u2013e639 (2022).","journal-title":"Circulation"},{"key":"83244_CR2","doi-asserted-by":"publisher","first-page":"8122","DOI":"10.1002\/jcp.27597","volume":"234","author":"FG Tahrir","year":"2019","unstructured":"Tahrir, F. G., Langford, D., Amini, S., MohseniAhooyi, T. & Khalili, K. Mitochondrial quality control in cardiac cells: Mechanisms and role in cardiac cell injury and disease. J. Cell Physiol. 234, 8122\u20138133 (2019).","journal-title":"J. Cell Physiol."},{"key":"83244_CR3","doi-asserted-by":"crossref","unstructured":"Duan, X., Liu, X. & Zhan, Z. Metabolic regulation of cardiac regeneration. Front. Cardiovasc. Med. 9 (2022).","DOI":"10.3389\/fcvm.2022.933060"},{"key":"83244_CR4","doi-asserted-by":"publisher","first-page":"6672","DOI":"10.1038\/s41467-022-33631-z","volume":"13","author":"X Zhou","year":"2022","unstructured":"Zhou, X. et al. Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction. Nat. Commun. 13, 6672 (2022).","journal-title":"Nat. Commun."},{"key":"83244_CR5","doi-asserted-by":"publisher","first-page":"107680","DOI":"10.1016\/j.exer.2019.05.020","volume":"186","author":"KM MirabitoColafella","year":"2019","unstructured":"MirabitoColafella, K. M., Bov\u00e9e, D. M. & Danser, A. H. J. The renin-angiotensin-aldosterone system and its therapeutic targets. Exp. Eye Res. 186, 107680 (2019).","journal-title":"Exp. Eye Res."},{"key":"83244_CR6","doi-asserted-by":"publisher","first-page":"9602","DOI":"10.3390\/ijms22179602","volume":"22","author":"N de las Heras","year":"2021","unstructured":"de las Heras, N. et al. Proanthocyanidins maintain cardiac ionic homeostasis in aldosterone-induced hypertension and heart failure. Int. J. Mol. Sci. 22, 9602 (2021).","journal-title":"Int. J. Mol. Sci."},{"key":"83244_CR7","doi-asserted-by":"publisher","first-page":"2785","DOI":"10.1093\/eurheartj\/ehm370","volume":"28","author":"S Ravassa","year":"2007","unstructured":"Ravassa, S. et al. Upregulation of myocardial Annexin A5 in hypertensive heart disease: Association with systolic dysfunction. Eur. Heart J. 28, 2785\u20132791 (2007).","journal-title":"Eur. Heart J."},{"key":"83244_CR8","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1152\/physrev.00030.2001","volume":"82","author":"V Gerke","year":"2002","unstructured":"Gerke, V. & Moss, S. E. Annexins: From structure to function. Physiol. Rev. 82, 331\u2013371 (2002).","journal-title":"Physiol. Rev."},{"key":"83244_CR9","doi-asserted-by":"publisher","first-page":"3439","DOI":"10.3390\/ijms22073439","volume":"22","author":"T Grewal","year":"2021","unstructured":"Grewal, T. et al. Annexin animal models\u2014From fundamental principles to translational research. Int. J. Mol. Sci. 22, 3439 (2021).","journal-title":"Int. J. Mol. Sci."},{"key":"83244_CR10","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1016\/j.cca.2022.05.009","volume":"531","author":"Y-Z Li","year":"2022","unstructured":"Li, Y.-Z. et al. Annexin A protein family in atherosclerosis. Clin.Chim. Acta 531, 406\u2013417 (2022).","journal-title":"Clin. Chim. Acta"},{"key":"83244_CR11","doi-asserted-by":"publisher","first-page":"525","DOI":"10.3390\/bios12070525","volume":"12","author":"A Pasquarelli","year":"2022","unstructured":"Pasquarelli, A. et al. Ultrasensitive diamond microelectrode application in the detection of Ca2+ transport by AnnexinA5-containing nanostructured liposomes. Biosensors 12, 525 (2022).","journal-title":"Biosensors"},{"key":"83244_CR12","doi-asserted-by":"publisher","first-page":"6753","DOI":"10.1038\/s41598-018-25143-y","volume":"8","author":"RCM de Jong","year":"2018","unstructured":"de Jong, R. C. M. et al. Annexin A5 reduces infarct size and improves cardiac function after myocardial ischemia-reperfusion injury by suppression of the cardiac inflammatory response. Sci. Rep-UK 8, 6753 (2018).","journal-title":"Sci. Rep-UK"},{"key":"83244_CR13","doi-asserted-by":"publisher","first-page":"2117","DOI":"10.1111\/jcmm.12374","volume":"18","author":"M Burgmaier","year":"2014","unstructured":"Burgmaier, M. et al. AnxA5 reduces plaque inflammation of advanced atherosclerotic lesions in apoE(-\/-) mice. J. Cell Mol. Med. 18, 2117\u20132124 (2014).","journal-title":"J. Cell Mol. Med."},{"key":"83244_CR14","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1111\/joim.12396","volume":"279","author":"LJ Schurgers","year":"2016","unstructured":"Schurgers, L. J. et al. Circulating annexin A5 predicts mortality in patients with heart failure. J. Intern. Med. 279, 89\u201397 (2016).","journal-title":"J. Intern. Med."},{"key":"83244_CR15","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1097\/HJH.0000000000001143","volume":"35","author":"A Maloberti","year":"2017","unstructured":"Maloberti, A. et al. Annexin A5 in treated hypertensive patients and its association with target organ damage. J. Hypertens. 35, 154\u2013161 (2017).","journal-title":"J. Hypertens."},{"key":"83244_CR16","doi-asserted-by":"publisher","first-page":"e8305","DOI":"10.1097\/MD.0000000000008305","volume":"96","author":"B Zhang","year":"2017","unstructured":"Zhang, B. et al. The association between annexin A5 (ANXA5) gene polymorphism and left ventricular hypertrophy (LVH) in Chinese endogenous hypertension patients. Medicine (Baltimore) 96, e8305 (2017).","journal-title":"Medicine (Baltimore)"},{"key":"83244_CR17","doi-asserted-by":"publisher","first-page":"7673","DOI":"10.1111\/jcmm.14640","volume":"23","author":"F-Y Huang","year":"2019","unstructured":"Huang, F.-Y. et al. The bifunctional SDF-1-AnxA5 fusion protein protects cardiac function after myocardial infarction. J. Cell Mol. Med. 23, 7673\u20137684 (2019).","journal-title":"J. Cell Mol. Med."},{"key":"83244_CR18","doi-asserted-by":"publisher","first-page":"9767","DOI":"10.1007\/s11033-022-07468-0","volume":"49","author":"S Ghafouri-Fard","year":"2022","unstructured":"Ghafouri-Fard, S. et al. Interplay between PI3K\/AKT pathway and heart disorders. Mol. Biol. Rep. 49, 9767\u20139781 (2022).","journal-title":"Mol. Biol. Rep."},{"key":"83244_CR19","doi-asserted-by":"publisher","first-page":"23","DOI":"10.3892\/ijmm.2020.4781","volume":"47","author":"AY Korshunova","year":"2021","unstructured":"Korshunova, A. Y. et al. BCL2-regulated apoptotic process in myocardial ischemia-reperfusion injury (Review). Int. J. Mol. Med. 47, 23\u201336 (2021).","journal-title":"Int. J. Mol. Med."},{"key":"83244_CR20","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1186\/s13619-021-00087-3","volume":"10","author":"T Liang","year":"2021","unstructured":"Liang, T., Gao, F. & Chen, J. Role of PTEN-less in cardiac injury, hypertrophy and regeneration. Cell Regen. 10, 25 (2021).","journal-title":"Cell Regen."},{"key":"83244_CR21","doi-asserted-by":"publisher","first-page":"2502","DOI":"10.1097\/HJH.0000000000003285","volume":"40","author":"S-Y Lu","year":"2022","unstructured":"Lu, S.-Y. et al. Angiotensin II prompts heart cell apoptosis via AT1 receptor-augmented phosphatase and tensin homolog and miR-320-3p functions to enhance suppression of the IGF1R-PI3K-AKT survival pathway. J. Hypertens. 40, 2502 (2022).","journal-title":"J. Hypertens."},{"key":"83244_CR22","doi-asserted-by":"publisher","first-page":"111022","DOI":"10.1016\/j.biopha.2020.111022","volume":"133","author":"C Ying","year":"2021","unstructured":"Ying, C. et al. Qingda granule attenuates angiotensin II-induced cardiac hypertrophy and apoptosis and modulates the PI3K\/AKT pathway. Biomed. Pharmacother. 133, 111022 (2021).","journal-title":"Biomed. Pharmacother."},{"key":"83244_CR23","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1007\/s00404-022-06524-1","volume":"307","author":"X Wang","year":"2023","unstructured":"Wang, X., Dai, Y., Zhang, J. & Li, X. Annexin A5 suppression promotes the progression of cervical cancer. Arch. Gynecol. Obstet. 307, 937\u2013943 (2023).","journal-title":"Arch. Gynecol. Obstet."},{"key":"83244_CR24","doi-asserted-by":"publisher","first-page":"2557","DOI":"10.3892\/or.2014.3547","volume":"32","author":"J Wang","year":"2014","unstructured":"Wang, J. et al. Annexin A5 inhibits diffuse large B-cell lymphoma cell invasion and chemoresistance through phosphatidylinositol 3-kinase signaling. Oncol. Rep. 32, 2557\u20132563 (2014).","journal-title":"Oncol. Rep."},{"key":"83244_CR25","unstructured":"NCD Risk Factor Collaboration (NCD-RisC). Worldwide trends in hypertension prevalence and progress in treatment and control from 1990 to 2019: a pooled analysis of 1201 population-representative studies with 104 million participants. Lancet 398, 957\u2013980 (2021)."},{"key":"83244_CR26","doi-asserted-by":"publisher","first-page":"2606","DOI":"10.3390\/jcm12072606","volume":"12","author":"V Nemtsova","year":"2023","unstructured":"Nemtsova, V., Vischer, A. S. & Burkard, T. Hypertensive heart disease: A narrative review series-part 1: Pathophysiology and microstructural changes. J. Clin. Med. 12, 2606 (2023).","journal-title":"J. Clin. Med."},{"key":"83244_CR27","doi-asserted-by":"publisher","first-page":"2208777","DOI":"10.1080\/10641963.2023.2208777","volume":"45","author":"K Jiao","year":"2023","unstructured":"Jiao, K., Su, P. & Li, Y. FGFR2 modulates the Akt\/Nrf2\/ARE signaling pathway to improve angiotensin II-induced hypertension-related endothelial dysfunction. Clin. Exp. Hypertens. 45, 2208777 (2023).","journal-title":"Clin. Exp. Hypertens."},{"key":"83244_CR28","doi-asserted-by":"publisher","first-page":"137","DOI":"10.59249\/NCST6937","volume":"96","author":"M Samad","year":"2023","unstructured":"Samad, M., Malempati, S. & Restini, C. B. A. Natriuretic peptides as biomarkers: Narrative review and considerations in cardiovascular and respiratory dysfunctions. Yale J. Biol. Med. 96, 137\u2013149 (2023).","journal-title":"Yale J. Biol. Med."},{"key":"83244_CR29","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1038\/labinvest.3780016","volume":"80","author":"D Benevolensky","year":"2000","unstructured":"Benevolensky, D. et al. Expression and localization of the annexins II, V, and VI in myocardium from patients with end-stage heart failure. Lab. Invest. 80, 123\u2013133 (2000).","journal-title":"Lab. Invest."},{"key":"83244_CR30","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1016\/j.cardiores.2004.11.010","volume":"65","author":"E Camors","year":"2005","unstructured":"Camors, E., Monceau, V. & Charlemagne, D. Annexins and Ca2+ handling in the heart. Cardiovasc. Res. 65, 793\u2013802 (2005).","journal-title":"Cardiovasc. Res."},{"key":"83244_CR31","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1016\/j.cardiores.2004.08.003","volume":"64","author":"V Monceau","year":"2004","unstructured":"Monceau, V. et al. Externalization of endogenous annexin A5 participates in apoptosis of rat cardiomyocytes. Cardiovasc Res 64, 496\u2013506 (2004).","journal-title":"Cardiovasc. Res."},{"key":"83244_CR32","doi-asserted-by":"publisher","first-page":"2437","DOI":"10.1016\/j.bpj.2013.03.060","volume":"104","author":"JW Gauer","year":"2013","unstructured":"Gauer, J. W. et al. Membrane modulates affinity for calcium ion to create an apparent cooperative binding response by annexin a5. Biophys J 104, 2437\u20132447 (2013).","journal-title":"Biophys. J."},{"key":"83244_CR33","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1152\/physrev.00020.2015","volume":"96","author":"EM Bevers","year":"2016","unstructured":"Bevers, E. M. & Williamson, P. L. Getting to the outer leaflet: Physiology of phosphatidylserine exposure at the plasma membrane. Physiol. Rev. 96, 605\u2013645 (2016).","journal-title":"Physiol. Rev."},{"key":"83244_CR34","doi-asserted-by":"publisher","first-page":"1000760","DOI":"10.3389\/fcell.2022.1000760","volume":"10","author":"N M\u00e9ndez-Barbero","year":"2022","unstructured":"M\u00e9ndez-Barbero, N., San Sebastian-Jaraba, I., Bl\u00e1zquez-Serra, R., Mart\u00edn-Ventura, J. L. & Blanco-Colio, L. M. Annexins and cardiovascular diseases: Beyond membrane trafficking and repair. Front. Cell Dev. Biol. 10, 1000760 (2022).","journal-title":"Front. Cell Dev. Biol."},{"key":"83244_CR35","doi-asserted-by":"publisher","first-page":"101634","DOI":"10.1016\/j.redox.2020.101634","volume":"36","author":"F Xu","year":"2020","unstructured":"Xu, F. et al. Annexin A5 regulates hepatic macrophage polarization via directly targeting PKM2 and ameliorates NASH. Redox Biol. 36, 101634 (2020).","journal-title":"Redox Biol."},{"key":"83244_CR36","doi-asserted-by":"publisher","first-page":"1839","DOI":"10.3892\/ijo.2017.3942","volume":"50","author":"J Tang","year":"2017","unstructured":"Tang, J. et al. High Annexin A5 expression promotes tumor progression and poor prognosis in renal cell carcinoma. Int. J. Oncol. 50, 1839\u20131847 (2017).","journal-title":"Int. J. Oncol."},{"key":"83244_CR37","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1093\/cvr\/cvq183","volume":"88","author":"S Ravassa","year":"2010","unstructured":"Ravassa, S. et al. Cardiac resynchronization therapy-induced left ventricular reverse remodelling is associated with reduced plasma annexin A5. Cardiovasc. Res. 88, 304\u2013313 (2010).","journal-title":"Cardiovasc. Res."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-83244-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-83244-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-83244-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T09:10:35Z","timestamp":1735636235000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-024-83244-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,30]]},"references-count":37,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["83244"],"URL":"https:\/\/doi.org\/10.1038\/s41598-024-83244-3","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,30]]},"assertion":[{"value":"15 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"All animal experiments were approved by the Animal Ethics Committee of the Guizhou Medical University (Permit No.1900572).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"31915"}}