{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T01:23:01Z","timestamp":1769822581204,"version":"3.49.0"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Fujian Provincial Natural Science Foundation","doi-asserted-by":"crossref","award":["2022J011513"],"award-info":[{"award-number":["2022J011513"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003392","name":"Fujian Provincial Natural Science Foundation","doi-asserted-by":"crossref","award":["2021J011452"],"award-info":[{"award-number":["2021J011452"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Qihang Foundation of Fujian Medical University","award":["2020QH1341"],"award-info":[{"award-number":["2020QH1341"]}]},{"name":"Ningde Natural Science Foundation","award":["2022J010"],"award-info":[{"award-number":["2022J010"]}]},{"name":"Ningde Natural Science Foundation","award":["2022J04"],"award-info":[{"award-number":["2022J04"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"DOI":"10.1038\/s41598-025-01340-4","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T11:31:22Z","timestamp":1749209482000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prolonged irrigation time in endoscopic aqueous medium impairs MSC\/\u03b2-TCP adhesion and osteogenic potential"],"prefix":"10.1038","volume":"15","author":[{"given":"Wugui","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangfeng","family":"Ling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengshou","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hengmei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kongning","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shijie","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchao","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengzhao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"issue":"6","key":"1340_CR1","doi-asserted-by":"publisher","first-page":"1022","DOI":"10.31616\/asj.2022.0435","volume":"16","author":"YH Kim","year":"2022","unstructured":"Kim, Y. H. et al. Lumbar interbody fusion and osteobiologics for lumbar fusion. Asian Spine J. 16(6), 1022 (2022).","journal-title":"Asian Spine J."},{"issue":"3","key":"1340_CR2","doi-asserted-by":"publisher","first-page":"163","DOI":"10.3928\/01477447-20220128-07","volume":"45","author":"JH Eum","year":"2022","unstructured":"Eum, J. H. et al. Endoscopic extreme transforaminal lumbar interbody fusion with large spacers: A technical note and preliminary report. Orthopedics 45(3), 163\u2013168 (2022).","journal-title":"Orthopedics"},{"issue":"5","key":"1340_CR3","doi-asserted-by":"publisher","first-page":"1157","DOI":"10.4103\/0028-3886.299145","volume":"68","author":"HS Kim","year":"2020","unstructured":"Kim, H. S., Wu, P. H., Raorane, H. D. & Jang, I. T. Generation change of practice in spinal surgery: Can endoscopic spine surgery expand its indications to fill in the role of conventional open spine surgery in most of degenerative spinal diseases and disc herniations: A study of 616 spinal cases 3 years. Neurol. India 68(5), 1157\u20131165 (2020).","journal-title":"Neurol. India"},{"issue":"4","key":"1340_CR4","doi-asserted-by":"publisher","first-page":"1224","DOI":"10.14245\/ns.2346888.444","volume":"20","author":"P Pholprajug","year":"2023","unstructured":"Pholprajug, P., Kotheeranurak, V., Liu, Y. & Kim, J. S. The endoscopic lumbar interbody fusion: A narrative review, and future perspective. Neurospine 20(4), 1224 (2023).","journal-title":"Neurospine"},{"issue":"1","key":"1340_CR5","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1186\/s12891-024-08146-x","volume":"25","author":"Y Ding","year":"2024","unstructured":"Ding, Y. et al. Comparison of efficacy and safety between unilateral biportal endoscopic transforaminal lumbar interbody fusion versus uniportal endoscopic transforaminal lumbar interbody fusion for the treatment of lumbar degenerative diseases: A systematic review and meta-analysis. BMC Musculoskeletal Disord. 25(1), 1037 (2024).","journal-title":"BMC Musculoskeletal Disord."},{"key":"1340_CR6","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1016\/j.wneu.2024.06.147","volume":"189","author":"D Nakajima","year":"2024","unstructured":"Nakajima, D. & Lim, Wu. Full-endoscopic posterior lumbar interbody fusion: A review and technical note. World Neurosurg. 189, 418\u2013427 (2024).","journal-title":"World Neurosurg."},{"issue":"29","key":"1340_CR7","doi-asserted-by":"publisher","first-page":"3973","DOI":"10.1016\/j.biomaterials.2008.06.026","volume":"29","author":"Y Gan","year":"2008","unstructured":"Gan, Y. et al. The clinical use of enriched bone marrow stem cells combined with porous beta-tricalcium phosphate in posterior spinal fusion. Biomaterials 29(29), 3973\u20133982 (2008).","journal-title":"Biomaterials"},{"issue":"5","key":"1340_CR8","doi-asserted-by":"publisher","first-page":"389","DOI":"10.3390\/gels9050389","volume":"9","author":"F Re","year":"2023","unstructured":"Re, F., Borsani, E., Rezzani, R., Sartore, L. & Russo, D. Bone regeneration using mesenchymal stromal cells and biocompatible scaffolds: A concise review of the current clinical trials. Gels 9(5), 389 (2023).","journal-title":"Gels"},{"issue":"6","key":"1340_CR9","doi-asserted-by":"publisher","first-page":"686","DOI":"10.3109\/14653249.2012.677821","volume":"14","author":"M Pierini","year":"2012","unstructured":"Pierini, M. et al. Efficient isolation and enrichment of mesenchymal stem cells from bone marrow. Cytotherapy 14(6), 686\u2013693 (2012).","journal-title":"Cytotherapy"},{"issue":"4","key":"1340_CR10","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1177\/1947603518768080","volume":"10","author":"B Gaul","year":"2019","unstructured":"Gaul, B. & Hoenecke, D. A review of commercially available point-of-care devices to concentrate bone marrow for the treatment of osteoarthritis and focal cartilage lesions. Cartilage 10(4), 387\u2013394 (2019).","journal-title":"Cartilage"},{"issue":"7","key":"1340_CR11","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1016\/j.jcyt.2024.02.007","volume":"26","author":"F Sanchez-Guijo","year":"2024","unstructured":"Sanchez-Guijo, F. et al. Current challenges in cell and gene therapy: A joint view from the European committee of the international society for cell & gene therapy (ISCT) and the European society for blood and marrow transplantation (EBMT). Cytotherapy 26(7), 681\u2013685 (2024).","journal-title":"Cytotherapy"},{"issue":"1","key":"1340_CR12","doi-asserted-by":"publisher","first-page":"15463","DOI":"10.1038\/s41598-017-15451-0","volume":"7","author":"Y Zhuang","year":"2017","unstructured":"Zhuang, Y. et al. A novel cytotherapy device for rapid screening, enriching and combining mesenchymal stem cells into a biomaterial for promoting bone regeneration. Sci. Rep. 7(1), 15463 (2017).","journal-title":"Sci. Rep."},{"issue":"1","key":"1340_CR13","doi-asserted-by":"publisher","first-page":"4715","DOI":"10.1038\/s41598-025-86776-4","volume":"15","author":"B Ding","year":"2025","unstructured":"Ding, B., Wang, X., Zhao, C. & Zhao, J. Rapid preparation of bioactive composites for transforaminal lumbar interbody fusion. Sci. Rep. 15(1), 4715 (2025).","journal-title":"Sci. Rep."},{"key":"1340_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13287-018-0906-1","volume":"9","author":"W Chu","year":"2018","unstructured":"Chu, W. et al. Mesenchymal stem cells and porous \u03b2-tricalcium phosphate composites prepared through stem cell screen-enrich-combine (\u2212 biomaterials) circulating system for the repair of critical size bone defects in goat tibia. Stem Cell Res. Ther. 9, 1\u201312 (2018).","journal-title":"Stem Cell Res. Ther."},{"key":"1340_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12967-019-02131-y","volume":"17","author":"W Chu","year":"2019","unstructured":"Chu, W. et al. Comparison and characterization of enriched mesenchymal stem cells obtained by the repeated filtration of autologous bone marrow through porous biomaterials. J. Transl. Med. 17, 1\u201316 (2019).","journal-title":"J. Transl. Med."},{"issue":"6","key":"1340_CR16","doi-asserted-by":"publisher","first-page":"454","DOI":"10.21037\/atm-20-6689","volume":"9","author":"W Chu","year":"2021","unstructured":"Chu, W. et al. Use of a novel screen\u2013enrich\u2013combine (-biomaterials) circulating system to fill a 3D-printed open Ti6Al4V frame with mesenchymal stem cells\/\u03b2-tricalcium phosphate to repair complex anatomical bone defects in load-bearing areas. Ann. Transl. Med. 9(6), 454 (2021).","journal-title":"Ann. Transl. Med."},{"issue":"2","key":"1340_CR17","doi-asserted-by":"publisher","first-page":"177","DOI":"10.21182\/jmisst.2023.00997","volume":"8","author":"Y Nakajima","year":"2023","unstructured":"Nakajima, Y., Takaoki, K., Akahori, S., Motomura, A. & Ohara, Y. A review of fully endoscopic lumbar interbody fusion. J. Minim. Invas. Spine Surg. Techn. 8(2), 177\u2013185 (2023).","journal-title":"J. Minim. Invas. Spine Surg. Techn."},{"key":"1340_CR18","doi-asserted-by":"publisher","first-page":"e475","DOI":"10.1016\/j.wneu.2020.08.204","volume":"144","author":"Y Li","year":"2020","unstructured":"Li, Y. et al. Full-endoscopic posterior lumbar interbody fusion via an interlaminar approach versus minimally invasive transforaminal lumbar interbody fusion: A preliminary retrospective Study. World Neurosurg. 144, e475\u2013e482 (2020).","journal-title":"World Neurosurg."},{"issue":"1","key":"1340_CR19","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1186\/s13018-025-05632-3","volume":"20","author":"L Yang","year":"2025","unstructured":"Yang, L. et al. Full-endoscopic posterior lumbar interbody fusion for the treatment of lumbar degenerative diseases: A technical note with 2-year follow-up. J. Orthop. Surg. Res. 20(1), 286 (2025).","journal-title":"J. Orthop. Surg. Res."},{"issue":"04","key":"1340_CR20","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1055\/s-0041-1730970","volume":"83","author":"Y Ishihama","year":"2022","unstructured":"Ishihama, Y. et al. Full-endoscopic trans-kambin triangle lumbar interbody fusion: Surgical technique and nomenclature. J. Neurol. Surg. Part A: Central Eur. Neurosurg. 83(04), 308\u2013313 (2022).","journal-title":"J. Neurol. Surg. Part A: Central Eur. Neurosurg."},{"issue":"s4","key":"1340_CR21","doi-asserted-by":"publisher","first-page":"S71","DOI":"10.14444\/7168","volume":"14","author":"C Sharma","year":"2021","unstructured":"Sharma, C. & Jain, S. Full endoscopic lumbar transforaminal interbody fusion in DDD lumbar degenerative disc disease: A latest technique. Int. J. Spine Surg. 14(s4), S71\u2013S77 (2021).","journal-title":"Int. J. Spine Surg."},{"issue":"1","key":"1340_CR22","doi-asserted-by":"publisher","first-page":"4","DOI":"10.3390\/ma12010004","volume":"12","author":"MM Ferreira","year":"2018","unstructured":"Ferreira, M. M. et al. Doping \u03b2-TCP as a strategy for enhancing the regenerative potential of composite \u03b2-TCP: Alkali-free bioactive glass bone grafts. Experimental study in rats. Materials 12(1), 4 (2018).","journal-title":"Materials"},{"issue":"1","key":"1340_CR23","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1186\/s13018-022-03091-8","volume":"17","author":"Y Yu","year":"2022","unstructured":"Yu, Y., Robinson, D. L., Ackland, D. C., Yang, Y. & Lee, P. V. S. Influence of the geometric and material properties of lumbar endplate on lumbar interbody fusion failure: A systematic review. J. Orthop. Surg. Res. 17(1), 224 (2022).","journal-title":"J. Orthop. Surg. Res."},{"key":"1340_CR24","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.jocn.2019.10.001","volume":"70","author":"Hu Ye","year":"2019","unstructured":"Ye, Hu. & Zhang, Xu. Impact of lumbar interbody fusion surgery on postoperative outcomes in patients with recurrent lumbar disc herniation: Analysis of the US national inpatient sample. J. Clin. Neurosci. 70, 20\u201326 (2019).","journal-title":"J. Clin. Neurosci."},{"key":"1340_CR25","doi-asserted-by":"publisher","first-page":"213738","DOI":"10.1016\/j.bioadv.2023.213738","volume":"157","author":"Q Gao","year":"2024","unstructured":"Gao, Q. et al. Biomaterials regulates BMSCs differentiation via mechanical microenvironment. Biomater. Adv. 157, 213738 (2024).","journal-title":"Biomater. Adv."},{"issue":"8","key":"1340_CR26","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.3390\/cells10081925","volume":"10","author":"K Stamnitz","year":"2021","unstructured":"Stamnitz, K. Mesenchymal stem cells, bioactive factors, and scaffolds in bone repair: From research perspectives to clinical practice. Cells 10(8), 1925 (2021).","journal-title":"Cells"},{"issue":"1","key":"1340_CR27","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1007\/s00586-020-06596-0","volume":"30","author":"B Meng","year":"2021","unstructured":"Meng, B. & Burton, W. Lumbar interbody fusion: Recent advances in surgical techniques and bone healing strategies. Eur. Spine J. 30(1), 22\u201333 (2021).","journal-title":"Eur. Spine J."},{"issue":"10","key":"1340_CR28","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1093\/stcltm\/szac052","volume":"11","author":"S Cho","year":"2022","unstructured":"Cho, S. et al. Preclinical study of human bone marrow-derived mesenchymal stem cells using a 3-dimensional manufacturing setting for enhancing spinal fusion. Stem Cells Transl. Med. 11(10), 1072\u20131088 (2022).","journal-title":"Stem Cells Transl. Med."},{"key":"1340_CR29","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.ijsu.2020.02.043","volume":"76","author":"S Ao","year":"2020","unstructured":"Ao, S. et al. Comparison of Preliminary clinical outcomes between percutaneous endoscopic and minimally invasive transforaminal lumbar interbody fusion for lumbar degenerative diseases in a tertiary hospital: Is percutaneous endoscopic procedure superior to MIS-TLIF? A prospective cohort study. Int. J. Surg. 76, 136\u2013143 (2020).","journal-title":"Int. J. Surg."},{"issue":"1","key":"1340_CR30","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1007\/s10143-024-02337-9","volume":"47","author":"X Shen","year":"2024","unstructured":"Shen, X. et al. Early result of percutaneous full-endoscopic transforaminal lumbar interbody fusion in the treatment of single-level lumbar degenerative diseases: A retrospective study. Neurosurg. Rev. 47(1), 115 (2024).","journal-title":"Neurosurg. Rev."},{"key":"1340_CR31","doi-asserted-by":"publisher","first-page":"100861","DOI":"10.1016\/j.mtbio.2023.100861","volume":"23","author":"M Ghasemzadeh-Hasankolaei","year":"2023","unstructured":"Ghasemzadeh-Hasankolaei, M., Pinto, C. A., Jesus, D., Saraiva, J. A. & Mano, J. F. Effect of high cyclic hydrostatic pressure on osteogenesis of mesenchymal stem cells cultured in liquefied micro-compartments. Mater. Today Bio 23, 100861 (2023).","journal-title":"Mater. Today Bio"},{"issue":"4","key":"1340_CR32","doi-asserted-by":"publisher","first-page":"2300714","DOI":"10.1002\/biot.202300714","volume":"19","author":"Y Zhou","year":"2024","unstructured":"Zhou, Y. et al. Fluid shear force and hydrostatic pressure jointly promote osteogenic differentiation of BMSCs by activating YAP1 and NFAT2. Biotechnol. J. 19(4), 2300714 (2024).","journal-title":"Biotechnol. J."},{"key":"1340_CR33","doi-asserted-by":"publisher","first-page":"102715","DOI":"10.1016\/j.tice.2024.102715","volume":"93","author":"F Abbasloo","year":"2025","unstructured":"Abbasloo, F., Vahidi, B., Khani, M. M., Sigaroodi, F. & Sarbandi, R. R. Osteogenic differentiation of mesenchymal stem cell on poly sorbitol sebacate scaffold under shear stress in a bioreactor. Tissue Cell 93, 102715 (2025).","journal-title":"Tissue Cell"},{"issue":"12","key":"1340_CR34","doi-asserted-by":"publisher","first-page":"1845","DOI":"10.4252\/wjsc.v13.i12.1845","volume":"13","author":"MS Choudhery","year":"2021","unstructured":"Choudhery, M. S. Strategies to improve regenerative potential of mesenchymal stem cells. World J. Stem Cells 13(12), 1845 (2021).","journal-title":"World J. Stem Cells"},{"key":"1340_CR35","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13287-018-1007-x","volume":"9","author":"B Antebi","year":"2018","unstructured":"Antebi, B. et al. Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cells. Stem Cell Res. Ther. 9, 1\u201315 (2018).","journal-title":"Stem Cell Res. Ther."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-01340-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-01340-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-01340-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T11:31:23Z","timestamp":1749209483000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-025-01340-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,6]]},"references-count":35,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["1340"],"URL":"https:\/\/doi.org\/10.1038\/s41598-025-01340-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,6]]},"assertion":[{"value":"4 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 May 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 June 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Consent for publication was obtained from the patient and approved by all the authors.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication (from patients\/participants)"}}],"article-number":"19976"}}