{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:58:44Z","timestamp":1789171124769,"version":"build-2803163510"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Jinshan Hospital of Fudan University","award":["JYQN-LC-202108"],"award-info":[{"award-number":["JYQN-LC-202108"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chronic neuropathic pain (CNP) is a debilitating complication of herpes zoster (HZ) with significant impact on quality of life. This study aimed to investigate the association between excitatory amino acid transporter 2 (EAAT-2) expression and plasma glutamate concentrations in HZ patients with CNP. This study was conducted with 102 consecutive patients diagnosed with HZ. Participants were divided into two groups: CNP (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200951) and acute pain (ACP,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200951). Pain severity was assessed using the Numerical Rating Scale. Blood samples were collected for genotype analysis, mRNA and protein extraction, and plasma glutamate measurement. EAAT-2 DNA genotyping was analyzed by polymerase chain reaction (PCR); EAAT-2 mRNA expression was analyzed by quantitative real-time PCR; EAAT-2 protein and glutamate levels were analyzed by enzyme-linked immunosorbent assay. The EAAT-2 DNA showed no significant difference in CNP and ACP patients. CNP patients exhibited lower EAAT-2 mRNA and protein levels compared to ACP patients. However, plasma glutamate levels were significantly elevated in the CNP patients. A correlation was observed between EAAT-2 protein concentration and plasma glutamate levels in the CNP group. This study demonstrates EAAT-2 mRNA downregulation, reduced EAAT-2 protein concentration, and elevated plasma glutamate levels play roles in CNP following HZ infection. These findings suggests that EAAT-2 may be a relevant target for further investigation in therapeutic development.\n                  <\/jats:p>","DOI":"10.1038\/s41598-025-01501-5","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T11:36:07Z","timestamp":1751369767000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Downregulation of EAAT-2 impairs chronic neuropathic pain via increasing of plasma glutamate after herpes zoster infection"],"prefix":"10.1038","volume":"15","author":[{"given":"Li-Na","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Hong","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu-Shuo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"issue":"2","key":"1501_CR1","doi-asserted-by":"publisher","first-page":"192","DOI":"10.3390\/v14020192","volume":"14","author":"A Patil","year":"2022","unstructured":"Patil, A., Goldust, M. & Wollina, U. 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