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High-quality reference genomes from geographically distant populations are essential to deepen our understanding of the biology, evolution, and genetic variation of this important malaria vector<jats:bold>.<\/jats:bold> In this study, we applied long-read PacBio and short-read Illumina sequencing technologies to assemble the complete genomes of two reference strains of <jats:italic>An. albimanus,<\/jats:italic> Stecla (originating from El Salvador), and Cartagena (originating from Colombia); and investigated the structural features of these genomes, including gene content, transposable elements (TEs), genetic variation, and structural rearrangements. Our hybrid assembly approach generated reference-quality genomes for each strain and recovered\u2009~\u200996% of the expected genome size. The genome assemblies of Stecla and Cartagena consisted of 109 and 149 scaffolds, with estimated genome sizes of 167.5 Mbp (N<jats:sub>50<\/jats:sub>\u2009=\u200988 Mbp) and 167.1 Mbp (N<jats:sub>50<\/jats:sub>\u2009=\u200987 Mbp), respectively. They exhibited a high level of completeness and contained a smaller number of gaps and ambiguous bases than either of the two previously published reference genomes for this species, suggesting a considerable improvement in the quality and completeness of the assemblies. A total of 12,082 and 12,120 protein-coding genes were predicted in Stecla and Cartagena, respectively. TE analyses indicated more repetitive content was captured in the long read assemblies. The assembled genomes shared 98.12% pairwise identity and synteny analyses suggested that gene position was conserved between both strains. These newly assembled genomes will serve as an important resource for future research in comparative genomics, proteomics, epigenetics, transcriptomics, and functional analysis of this important malaria vector.<\/jats:p>","DOI":"10.1038\/s41598-025-01713-9","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T14:49:19Z","timestamp":1748962159000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Chromosome-scale genome assembly and annotation of two geographically distinct strains of malaria vector Anopheles albimanus"],"prefix":"10.1038","volume":"15","author":[{"given":"Dieunel","family":"Derilus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gareth D.","family":"Weedall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W.","family":"Vandewege","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhwani","family":"Batra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mili","family":"Sheth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori A.","family":"Rowe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananias A.","family":"Escalante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Lenhart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy Mackenzie","family":"Impoinvil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,3]]},"reference":[{"key":"1713_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/1476-072X-11-13","volume":"11","author":"DO Fuller","year":"2012","unstructured":"Fuller, D. 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