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As an adjunct to periodontal therapy, probiotic administration has shown promising effects on oral health by decreasing pathogen counts and altering the immune response. This study thus investigates changes in morphology and remodelling of alveolar bone and periodontal ligament (PDL) due to an AN-like condition, along with potential protective effects of probiotics, using a rat model. Three-week-old female Wistar rats were divided into three groups: a control group with ad libitum food and two groups undergoing an activity-based anorexia (ABA) model. One ABA group received oral multi-strain probiotics during starvation. After five weeks, all rats were sacrificed for ex-vivo micro-CT scans of the maxilla and mandible to assess alveolar bone and PDL morphology, as well as histological evaluations for PDL fibre vitality and structural organization. The data were statistically evaluated by one-way ANOVA with Tukey\u2019s post-hoc test or by Kruskal\u2013Wallis with Dunn\u2019s test for parametric or nonparametric data, respectively. The results showed no structural changes in alveolar bone caused by either ABA or probiotic treatment; however, the ABA group exhibited a significant reduction in PDL thickness, which could not be reversed by probiotic treatment. Despite this, histological analysis indicated improved connectivity and density of PDL fibres in the probiotic group compared with the ABA-only group. No differences were found between the mandible and maxilla. In conclusion, while probiotics did not prevent PDL thinning, they enhanced its composition\/vitality compared to the ABA condition alone.<\/jats:p>","DOI":"10.1038\/s41598-025-02610-x","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T10:53:50Z","timestamp":1748861630000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preventive effect of probiotics on periodontal ligament in a rat model of anorexia nervosa"],"prefix":"10.1038","volume":"15","author":[{"given":"Marta","family":"Rizk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"H\u00e4ck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Brenji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Knaup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Niederau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Kiessling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Marx","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Moellmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Kahles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Pufe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Apel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kern","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Seitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"K\u00e4ver","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Voelz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Trinh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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